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LysoView™ Dyes

LysoView™ fluorescent dyes label lysosomes in live cells without a wash step, and are available in multiple colors with options for super-resolution imaging.

Product Attributes

Probe cellular localization

Lysosomes

For live or fixed cells

For live/intact cells

Assay type/options

Long term staining (24-72h), No-wash staining, Real-time imaging

Cell permeability

Membrane permeant

Colors

Blue, Green, Orange, Red, Far-red, Near-infrared

Storage Conditions

Store at -10 to -35 °C, Protect from light

Dye
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Catalog #
price
Qty
Trial size (10 uL)
50 uL
Trial size (1 vial)
Set of 10 vials
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Product Description

LysoView™ dyes are useful for staining lysosomes in live cells.

  • No-wash, fluorescent staining of lysosomes in live cells
  • Non-toxic for real-time live-cell imaging
  • Lower background than LysoTracker® dyes
  • Dye options for super-resolution imaging
  • Color choices from blue to far-red, plus a unique NIR dye

LysoView™ dyes are fluorescent stains for imaging lysosome localization and morphology in live cells. The dyes accumulate in low pH organelles, resulting in highly specific staining without the need for a wash step. LysoView™ staining is non-toxic, and can imaged over the course of days, even after dye washout for some dye options (see the Product Information Sheet for more information).

LysoView™ 540 and LysoView™ 633 exhibit pH-sensitive fluorescence, further enhancing the specificity of staining. LysoView™ 488 has been validated in super-resolution imaging by SIM (Ref. 7), while LysoView™ 650 fluorophore is compatible with super-resolution imaging by SIM and STED.

LysoView™ 540 and LysoView™ 633 stain vacuoles in yeast. Staining of bacteria varies between dyes. See our Cellular Stains Table for details.

Note: LysoView™ 540 has limited photostability, and may not be suitable for all microscopy applications. LysoView™ 550 is a bright and more photostable choice.

Also see our unique UV-activatable Light-On LysoView™ 555, which is non-fluorescent until it is reversibly activated by UV light.

LysoView™ Dyes

ProductCatalog numberSizeAbs/Em (nm)
(pH ≤ 5)
Detection
channel
Features
LysoView™ 405,
1000X in DMSO
70066-T10 uL318, 400/ 464DAPI, Pacific
Blue™
Blue fluorescent
lysosome stain
7006650 uL
LysoView™ 488,
1000X in DMSO
70067-T10 uL496/526GFP, FITCGreen fluorescent dye
validated in SIM
7006750 uL
LysoView™ 540,
1000X in DMSO
70061-T10 uL540/634TRITC, Cy®3, PEOrange, pH-
sensitive fluorescence**
7006150 uL
LysoView™ 550,
1000X in DMSO
70083-T10 uL542/567TRITC, Cy®3, PEBright & photostable
orange dye
7008350 uL
LysoView™ 594,
1000X in DMSO
70084-T10 uL585/634Texas Red®Bright & photostable
red dye
7008450 uL
LysoView™ 633
(lyophilized solid)
70058-T1 vial*634/657Cy®5, APCFar-red, pH-sensitive fluorescence
7005810 vials*
LysoView™ 640,
1000X in DMSO
70085-T10 uL640/671Cy®5, APCBright & photostable
far-red dye
7008550 uL
LysoView™ 650,
1000X in DMSO
70059-T10 uL650/675Cy®5, APCPhotostable far-red dye
compatible with SIM, STED
7005950 uL
LysoView™ 680,
1000X in DMSO
70086-T10 uL674/711Cy®5.5Unique near-IR
lysosome stain
7008650 uL
*Each vial of LysoView™ 633 makes 100 uL of 1000X dye after reconstitution.
** LysoView™ 540 has limited photostability and may not be suitable for all microcopy applications.
Cy dye is a registered trademark of GE Healthcare; Pacific Blue is a trademark and Texas Red is a registered trademark of Thermo Fisher Scientific.
LysoTracker is a registered trademark of Thermo Fisher Scientific.

References

  1. Mol Ther (2016) 24(12), 2100-2108. doi:10.1038/mt.2016.179 (LysoView™ 633)
  2. Carbohydrate Polym (2016) 153, 160-168. doi: 10.1016/j.carbpol.2016.07.080 (LysoView™ 633)
  3. Leuk Res (2016) 42, 59-67. doi:10.1016/j.leukres.2016.01.007 (LysoView™ 633)
  4. bioRxiv (2019) doi: https://doi.org/10.1101/747014 (LysoView™ 633)
  5. Int J Pharm (2019) 565, 391-408. doi:10.1016/j.ijpharm.2019.05.009 (LysoView™ 633)
  6. Oncogene (2019) 38, 1432–1447. https://doi.org/10.1038/s41388-018-0507-6 (LysoView™ 633)
  7. Light Sci Apps (2020) 9, 11. https://doi.org/10.1038/s41377-020-0249-4 (LysoView™ 488; SIM)
  8. Spectrochimica Acta A (2020) 233, 118234. https://doi.org/10.1016/j.saa.2020.118234 (LysoView™ 633)
  9. Dyes Pigments (2021) 184, 108771. https://doi.org/10.1016/j.dyepig.2020.108771
    (LysoView™ 633)
  10. Angiogenesis (2021) 24, 657-676. https://doi.org/10.1007/s10456-021-09778-6
    (LysoView™ 633)
  11. Cell Death Dis (2021) 12, 704. https://doi.org/10.1038/s41419-021-03978-0 (LysoView™ 540)
  12. Cell Rep (2021) 36(4) 109434. https://doi.org/10.1016/j.celrep.2021.109434 (LysoView™ 633)
  13. Nanomaterials (2021) 11(7), 1837. https://doi.org/10.3390/nano11071837 (LysoView™ 405)
  14. Sci Adv (2021) 7(21) eabf0573. DOI: 10.1126/sciadv.abf0573 (LysoView™ 405)
  15. Research Square (preprint) (2021). DOI: 10.21203/rs.3.rs-279271/v1 (LysoView™ 488, LipidSpot™ 488; SIM)
 

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