Monoclonal anti Ku Holo (p70;83) (KU729)

BNUB0729-0
BNUB0729-1
string(39) "$120.00"
Price:
 from
  $120.00
Clear selection
Catalog #: BNUB0729
Selection:
 Multiple sizes
Category: .
>>> Order 5 or more and receive 10% off.

Product Description

Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Ku-Holo (p70;83) (KU729)

Clonality

Monoclonal

Isotype

IgG's

Clone

KU729

Molecular weight

70 kDa & 80 kDa

Synonyms
Entrez gene ID

2547 (p70); 7520 (p80)

SwissProt

P12956 (p70); P13010 (p80)

Unigene

292493 (p70); 388739 (p80)

Immunogen

Nuclear extract of human HL-60 cells

Species reactivity

Human, Primates

Applications

Flow cytometry, Immunofluorescence

Cellular localization

Nuclear

Application notes

Does not react with mouse, rat or chicken; others not known, Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunocytochemistry Acetone-fixed cells 0.25-0.5 ug/mL for 30 minutes at RT, Immunofluorescence 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

Human cancer K562, HL-60, HEp-2 or HeLa cells

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life