Propidium Iodide Buffer (50 ug/mL)
Propidium Iodide is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a cell population and also used as a counterstain in multicolor fluorescent imaging.
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Propidium iodide (PI) is a cell impermeable nucleic acid intercalating dye. Because PI is excluded from viable cells, it can be used to distinguish necrotic or late apoptotic cells with damaged plasma membranes from viable cells or early apoptotic cells with intact cell membranes. After entering cells and binding to DNA and RNA, the fluorescence of PI is enhanced 20- to 30-fold. PI can be used in flow cytometry and fluorescence microscopy. PI is also utilized as a counterstain in multicolor fluorescent imaging, but nuclear-specific staining requires digestion of cellular RNA.
- 50 ug/mL solution in PBS buffer
- Orange/red liquid
- Store at 4°C and protect from light
- λEx/λEm (DNA-bound) = 535/617 nm
- λEx/λEm (no DNA) = 493/636 nm
- C27H34I2N4
- MW: 668.4
- [25535-16-4]
We offer PI in a variety of formulations and in conjunction with our fluorescently labeled Annexin V.
Propidium Iodide Products
Product | Unit Size | Catalog Number |
---|---|---|
Propidium Iodide, 100 mg | 100 mg | 40016 |
Propidium Iodide, 1 mg/mL in water | 10 mL | 40017 |
Propidium Iodide Buffer, 50 ug/mL | 2 mL | 40048 |
CF488A Annexin V and PI Apoptosis Kit | 100 assays | 30061 |
Ethidium homodimer III and 7-AAD are alternative dead-cell selective stains. Also see our Live-or-Dye™ Fixable Viability Staining Kits.
References
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