Recombinant Human Vitamin D-Binding Protein Conjugates
Vitamin D-Binding Protein (Vitamin D-BP, also known as GC Globulin) binds monomeric G-actin in fixed and permeabilized cells. Available conjugated to a selection of bright and photostable CF® Dyes for fluorescence microscopy.
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Product Description
Recombinant Human Vitamin D-Binding Protein (Vitamin D-BP, also known as GC Globulin) binds monomeric G-actin (1-3). Fluorescent conjugates of Vitamin D-BP can be used to stain monomeric G-actin in fixed and permeabilized cells.
- Labels monomeric G-actin in fixed and permeabilized cells
- Can be co-stained with fluorescent phalloidin for comparing G-actin and F-actin distribution
- Developed and optimized for immunofluorescence
- Available with bright and photostable CF® Dyes, including near-infrared CF®740
Vitamin D-BP staining may also be used in combination with fluorescent phalloidin staining of F-actin to visualize the distribution of unpolymerized G-actin relative to actin filaments. Vitamin D-BP is more specific for staining G-actin compared to fluorescent conjugates of DNase-I, which binds to DNA in addition to G-actin. Biotium’s Recombinant Human Vitamin D-BP Conjugates are labeled with a selection of our bright and photostable CF® Dyes for fluorescence microscopy.
Superior CF® Dyes
Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Recombinant Human Vitamin D-Binding Protein Conjugates
Conjugation | Ex/Em | Size | Catalog No. | Dye Features |
---|---|---|---|---|
CF®488A | 490/516 nm | 1 mL (100 ug) | 70087 | CF®488A Features |
CF®594 | 593/615 nm | 1 mL (100 ug) | 70088 | CF®594 Features |
CF®640R | 642/663 nm | 1 mL (100 ug) | 70089 | CF®640R Features |
CF®647 | 652/668 nm | 1 mL (100 ug) | 70090 | CF®647 Features |
CF®740 | 742/767 nm | 1 mL (100 ug) | 70091 | CF®740 Features |
For staining F-actin, Biotium recommends ActinBrite™ High Affinity Phalloidin Conjugates which were designed to preserve strong F-actin binding over conventional phalloidin conjugates. With ActinBrite™, samples can be imaged after for a month or more (depending on the conjugate and mounting method)—making delayed imaging easier and more dependable.
Browse Biotium’s comprehensive catalog of fluorescent bioconjugates as well novel and classic organelle stains.
References
- J Biol Chem, 255(6), 2270(1980), S0021-9258(19)85885-4
- J Cell Biol, 123(1), 1(1993), 10.1083/jcb.123.1.1
- Biochim Biophys Acta, 1452(1), 12(1999), 10.1016/s0167-4889(99)00119-6