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Recombinant Human Vitamin D-Binding Protein Conjugates

Vitamin D-Binding Protein (Vitamin D-BP, also known as GC Globulin) binds monomeric G-actin in fixed and permeabilized cells. Available conjugated to a selection of bright and photostable CF® Dyes for fluorescence microscopy.

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Product Description

Recombinant Human Vitamin D-Binding Protein (Vitamin D-BP, also known as GC Globulin) binds monomeric G-actin (1-3). Fluorescent conjugates of Vitamin D-BP can be used to stain monomeric G-actin in fixed and permeabilized cells.

  • Labels monomeric G-actin in fixed and permeabilized cells
  • Can be co-stained with fluorescent phalloidin for comparing G-actin and F-actin distribution
  • Developed and optimized for immunofluorescence
  • Available with bright and photostable CF® Dyes, including near-infrared CF®740

Vitamin D-BP staining may also be used in combination with fluorescent phalloidin staining of F-actin to visualize the distribution of unpolymerized G-actin relative to actin filaments. Vitamin D-BP is more specific for staining G-actin compared to fluorescent conjugates of DNase-I, which binds to DNA in addition to G-actin. Biotium’s Recombinant Human Vitamin D-BP Conjugates are labeled with a selection of our bright and photostable CF® Dyes for fluorescence microscopy.

Superior CF® Dyes

Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Passaged chondrocytes stained with Rhodamine-Phalloidin and Vitamin D-binding protein CF®488 conjugate to visualize F-actin and G-actin. Passaged chondrocytes were exposed to latrunculin A (1uM) for 30 minutes, and were either fixed immediately in 4%PFA (top and middle row) or pre-solubilized, to remove cytoplasmic G-actin, with 0.1%Triton for 2 minutes prior to fixation in 4%PFA (bottom row). Latrunculin treatment reduced staining for Phalloidin (predominantly F-actin) and increased staining for Vitamin D-binding protein (predominantly G-actin) (see middle row). Pre-treatment with 0.1% Triton prior to PFA fixation reduced staining for Vitamin D-binding protein (see bottom row). Images provided from Parreno Laboratory, Department of Biological Sciences, University of Delaware.

Recombinant Human Vitamin D-Binding Protein Conjugates

ConjugationEx/EmSizeCatalog No.Dye Features
CF®488A490/516 nm1 mL (100 ug)70087CF®488A Features
CF®594593/615 nm1 mL (100 ug)70088CF®594 Features
CF®640R642/663 nm1 mL (100 ug)70089CF®640R Features
CF®647652/668 nm1 mL (100 ug)70090CF®647 Features
CF®740742/767 nm1 mL (100 ug)70091CF®740 Features

For staining F-actin, Biotium recommends ActinBrite™ High Affinity Phalloidin Conjugates which were designed to preserve strong F-actin binding over conventional phalloidin conjugates. With ActinBrite™, samples can be imaged after for a month or more (depending on the conjugate and mounting method)—making delayed imaging easier and more dependable.

Browse Biotium’s comprehensive catalog of fluorescent bioconjugates as well novel and classic organelle stains.

References

  1. J Biol Chem, 255(6), 2270(1980), S0021-9258(19)85885-4
  2. J Cell Biol, 123(1), 1(1993), 10.1083/jcb.123.1.1
  3. Biochim Biophys Acta, 1452(1), 12(1999), 10.1016/s0167-4889(99)00119-6
 

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