Azide, Alkyne, BCN, Tetrazine & TCO
Bioorthogonal labeling involves reactions between functional groups that do not react with native targets in the cell. Therefore bioorthogonal conjugation can be highly specific and controlled. We offer dyes with a variety of functional groups for bioorthogonal labeling. The original bioorthogonal chemistries require copper catalysis, which can cause oxidative damage to cells and proteins. Newer chemistries require lower copper concentrations or are copper-free, avoiding this problem.
Azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. Picolyl azide undergoes the same reaction but at a much lower copper concentration. Azides also can undergo Staudinger ligation with phosphine containing compounds. Alkynes can be used as monomers to copolymerize into labeled polymers. BCN reacts with azide and methyltetrazine reacts with TCO (trans-cyclooctene) by copper-free reactions.
We offer a wide selection of our bright and photostable CF® dyes or biotin with your choice of bioorthogonal functional groups. Learn more about CF® Dyes.
CF® Dye Alkyne
92086, 92087, 92088, 92089, 92091, 92093, 92095, 96004, 96005, 96006, - 92090View allHide
CF® Dye alkynes react with azide groups via copper-catalyzed bioorthogonal cycloaddition. Can be used to fluorescently detect or label azide groups on target molecules. CF® Dye alkynes can also be used as building blocks to form fluorescent polymers.
CF® Dye Azide
92080, 92081, 92082, 92083, 92084, 92085, 92092, 92094, 92119, 92180, 92181, 92182, - 96000View allHide
CF® Dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.
CF® Dye BCN
96070, 92075, 92076, 92077, 92078, 92079, 92113, 92114, 96026, 96027, 96058, - 96059View allHide
CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labeling reactions are also available.
CF® Dye Methyltetrazine
96028, 96029, 96030, 96031, 96032, 96033, 96034, 96035, 96036, 96037, - 96039View allHide
CF® Dye with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecules via copper-free click chemistry.
CF® Dye Picolyl Azide
92187, 92188, 92189, 92190, 92191, 96001, 96002, 96003, - 96007View allHide
CF® dye picolyl azide derivatives fluorescently label alkyne groups on biomolecules via the standard azide-alkyne cycloaddition, but at low copper concentrations.
CF® Dye TCO
96040, 96041, 96042, 96043, 96044, 96045, 96046, 96047, 96048, 96049, - 96051View allHide
CF® Dye with TCO (trans-cyclooctene) groups react with tetrazine-labeled molecules via copper-free click chemistry.
CF® Dye Tetrazine
96055, 96054, 96056, 96096, - 96097View allHide
CF® Dyes with a tetrazine group react with cyclooctene (TCO, methylcyclopropene) labeled molecules via copper-free click chemistry.
Biotin Methyltetrazine
Biotin with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecule via copper free click chemistry.
Biotin TCO
Biotin with TCO (trans-cyclooctene) reacts with tetrazine labeled molecule via copper free click chemistry.
Cyanine Dye Alkyne
92100, - 92101View allHide
Cyanine 555 and Cyanine 647 alkynes can be used for the analysis of O-GlcNAcylated proteins by 2D glyco-DIGE.
CF® Dye DBCO
96111, 96110, 96112, 96113, 96114, 96115, 96116, 96117, 96118, 96119, 96120, 96121, 96122, - 96123View allHide
CF® Dye with dibenzocyclooctyne (i.e., DBCO, ADIBO) reacts with azide via copper-free click chemistry by strain-promoted azide-alkyne cycloaddition (SPAAC).
Biotin Alkyne
Biotin alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition.
Biotin Azide
Biotin azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition.
Biotin BCN
Biotin BCN, an alternative to biotin labeled DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition.
Biotin Picolyl Azide
Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.
MTS-BCN
MTS-BCN is a fast-reacting and highly selective thiol-reactive label for attaching BCN to thiol groups on biomolecules. BCN, an alternative to DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition.