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The Full Spectrum

A Biotium Blog

EvaGreen® Dye: The Swiss Army Knife of qPCR
EvaGreen® Dye: The Swiss Army Knife of qPCR
March 19, 2024
Trace the evolution of PCR and qPCR techniques from their inception to the innovative use of ...
Trace the evolution of PCR and qPCR techniques from their inception to the innovative use of EvaGreen® Dye. This article explores how EvaGreen® enhances performance in various molecular biology ...
Julianne Davis, MScJulianne Davis, MSc
7 Tips for Selecting the Perfect Antibody
7 Tips for Selecting the Perfect Antibody
January 9, 2024
Searching for the right antibody for your experiment? Give yourself an edge by reading up on seven important variables everyone should consider ...
Julianne Davis, MScJulianne Davis, MSc
Tissue Frozen in Time
Tissue Frozen in Time
July 26, 2023
A summary of the pros, cons, and methods for DNA and RNA extraction from FFPE tissue for clinical diagnostic and research purposes.
Julianne Davis, MScJulianne Davis, MSc
Exosome-Based Liquid Biopsies: The Potential & the Road Ahead
Exosome-Based Liquid Biopsies: The Potential & the Road Ahead
October 12, 2022
An overview of the advantages of exosomes as an effective liquid biopsy tool, with a focus on oncological applications.
Julianne Davis, MScJulianne Davis, MSc
Scaling Down to Go Big: An Overview of the Lab-on-a-Chip
Scaling Down to Go Big: An Overview of the Lab-on-a-Chip
December 9, 2021
From modeling single synapses to multiple organs, lab-on-a-chip (LOC) technology has gained attention as a powerful tool for biological and ...
Julianne Davis, MScJulianne Davis, MSc
Optical Imaging in Tissue with Near-Infrared Dyes
Optical Imaging in Tissue with Near-Infrared Dyes
September 17, 2021
A deep dive into the science, current technologies, and future prospects of near-IR fluorescence for in vivo imaging.
Christopher Pratt, PhDChristopher Pratt, PhD
Bring Out Your Dead: v-PCR for Microbial Population and Diversity Studies
Bring Out Your Dead: v-PCR for Microbial Population and Diversity Studies
May 20, 2021
Have you tried v-PCR yet? In this blog post we explore the endless applications for v-PCR in microbiology, sanitation, and clinical research.
Christopher Pratt, PhDChristopher Pratt, PhD
Cell Viability- Where to Begin?
Cell Viability- Where to Begin?
April 8, 2021
Exploring the many tools and metrics for assessing cell viability, including assays for cytotoxicity, cellular activity, and apoptosis.
Eric Torres, PhDEric Torres, PhD
Bringing Light to Extracellular Vesicles
Bringing Light to Extracellular Vesicles
March 11, 2021
A deep dive into exosomes, their origins, methods for analysis, and tremendous potential for medicine and research.
Christopher Pratt, PhDChristopher Pratt, PhD
An Interview with STORM expert Ke Xu on Super-Resolution Microscopy
An Interview with STORM expert Ke Xu on Super-Resolution Microscopy
January 27, 2021
Dr. Ke Xu, Ph.D, a leading STORM scientist at UC Berkeley, discusses the challenges and prospects of super-resolution microscopy.
Eric Torres, PhDEric Torres, PhD
Seeing More and Brighter Colors with Tyramide Signal Amplification
Seeing More and Brighter Colors with Tyramide Signal Amplification
January 13, 2021
Let's discuss tyramide signal amplification (TSA), a powerful tool for spatial proteomics and producing bright and high-resolution images.
Christopher Pratt, PhDChristopher Pratt, PhD
Easy and Fun Science Experiments to Try with Kids at Home
Easy and Fun Science Experiments to Try with Kids at Home
December 9, 2020
Pique your child's scientific curiosity with these fun and easy ideas to explore DNA, proteins, and fluorescence. Hands-on science for grade K-12.
Jacqueline Steenhuis, PhDJacqueline Steenhuis, PhD
Background Reducers for Improved Fluorescent Stains
Background Reducers for Improved Fluorescent Stains
October 9, 2020
Issues with high immunofluorescence background? Check out the best tools to reduce background and improve image quality.
Christopher Pratt, PhDChristopher Pratt, PhD
Getting the Whole Picture with Spectral Flow Cytometry
Getting the Whole Picture with Spectral Flow Cytometry
August 26, 2020
A brief overview of the exciting technology behind spectral cytometry, and how it's advancing the field of flow cytometry.
Christopher Pratt, PhDChristopher Pratt, PhD
Blowing Up the Microscopic with Expansion Microscopy
Blowing Up the Microscopic with Expansion Microscopy
July 29, 2020
Biological structures too small to image? Blow it up instead! Chris Pratt, PhD explores a fascinating imaging technique known as expansion microscopy.
Christopher Pratt, PhDChristopher Pratt, PhD
Pushing Biology Forward with Super Resolution
Pushing Biology Forward with Super Resolution
July 8, 2020
Our resident blog writer Christopher Pratt, PhD explores super-resolution microscopy and the creative tricks used to see beyond the diffraction limit.
Christopher Pratt, PhDChristopher Pratt, PhD
Luminescence: Mechanisms and Applications for 4 Prominent Techniques
Luminescence: Mechanisms and Applications for 4 Prominent Techniques
June 8, 2020
An overview of luminescent techniques used in biological research including: bioluminescence, fluorescence, phosphorescence, and chemiluminescence.
Nisha Cooch, PhDNisha Cooch, PhD
An Overview of Fluorescence Microscopy
An Overview of Fluorescence Microscopy
May 6, 2020
Learn more about how fluorescence microscopes work as well as specialized variants that have advanced modern biological research.
Gopal Ramakrishnan, PhDGopal Ramakrishnan, PhD
CF® Dyes. What started it all? Part 2. The Chemistry of Fluorescence
CF® Dyes. What started it all? Part 2. The Chemistry of Fluorescence
March 3, 2020
In the final entry of our 2-part blog series, we’ll look at how CF® Dyes are the next evolutionary step in fluorescent probes.
Eric Torres, PhDEric Torres, PhD
What Does Vaping Vitamin E Acetate Do To Your Lungs?
What Does Vaping Vitamin E Acetate Do To Your Lungs?
January 15, 2020
Lets explore the science behind vitamin E acetate as a vaping additive and the potential link to the EVALI outbreak.
Eric Torres, PhDEric Torres, PhD
CF® Dyes. What started it all? Part 1. A History of Fluorescence
CF® Dyes. What started it all? Part 1. A History of Fluorescence
November 22, 2019
Where did FITC, rhodamine, and Alexa Fluor® dyes come from? Find out in part 1 of this 2-part blog series on the history of fluorescent dyes.
Eric Torres, PhDEric Torres, PhD
Your Go-To Guide for Protein Stains
Your Go-To Guide for Protein Stains
September 10, 2019
Wondering if you should use CBB or silver stain for that gel? Find out with this comprehensive guide on protein stains!
Eric Torres, PhDEric Torres, PhD
Microbe Puzzle
Microbe Puzzle
June 18, 2019
Bored waiting for your cells to grow? Kill time and test your knowledge with this Microbe Acrostic puzzle!
Lori Roberts, PhDLori Roberts, PhD
Happy anniversary, NucView®! A decade of real-time apoptosis detection
Happy anniversary, NucView®! A decade of real-time apoptosis detection
November 19, 2018
A look back at NucView®, the apoptosis tool scientists were waiting for.
Alexis Madrid, PhDAlexis Madrid, PhD
The Application of Toxins and Poisons in Cell and Molecular Biology
The Application of Toxins and Poisons in Cell and Molecular Biology
October 31, 2018
Natural substances that are very bad for eating, but great for research!
Jeff Jung, Scientist IJeff Jung, Scientist I
The Chemist Tool Kit
The Chemist Tool Kit
September 17, 2018
Let's explore the key lab instruments used to make fluorescent dyes.
Jeff Jung, Scientist IJeff Jung, Scientist I
Making Colors In The Chem Lab
Making Colors In The Chem Lab
July 18, 2018
Curious about what it's like to make fluorescent dyes in the lab? Our expert dye chemist Patrick McGarraugh provides some colorful insight.
Patrick McGarraugh, PhDPatrick McGarraugh, PhD
12 years later: Viability PCR using PMA and PMAxx™ dyes
12 years later: Viability PCR using PMA and PMAxx™ dyes
June 1, 2018
Let's take a look back on the many uses of viability PCR in basic research, diagnostics and food safety over the years.
Alexis Madrid, PhDAlexis Madrid, PhD
Safe and Eco-friendly Gel Stains (Happy Earth Day!)
Safe and Eco-friendly Gel Stains (Happy Earth Day!)
May 2, 2018
Check out these non-toxic and non-hazardous alternatives to ethidium bromide and other mutagenic DNA gel stains.
Saloni Pasta, PhDSaloni Pasta, PhD
On the lookout for artifacts from DAPI and Hoechst in fluorescence microscopy
On the lookout for artifacts from DAPI and Hoechst in fluorescence microscopy
March 21, 2018
Photoconversion and cross-talk from blue nuclear dyes can ruin your image. Check out these tips on how to avoid it.
Lori Roberts, PhDLori Roberts, PhD