Drop-n-Stain™ CF® Dye Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed
CF® dye labeled, highly cross-adsorbed, donkey anti-rabbit IgG (H+L) secondary antibody provided in a convenient dropper bottle.
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Product Description
This is a CF® dye labeled, highly cross-adsorbed, donkey anti-rabbit IgG (H L) secondary antibody provided in a convenient dropper bottle for easy dispensing. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum.
- Highly cross-adsorbed for specific staining with minimal background
- Convenient dropper bottle for easy dispensing without pipetting
- Available with 4 bright and photostable CF® dyes
- Suitable for western, immunofluorescence, and immunohistology
Drop-n-Stain™ CF® Dye Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed
Conjugation | Ex/Em | Size | Catalog No. | Dye Features |
---|---|---|---|---|
CF®488A | 490/515 nm | 5 mL | 20950 | CF®488A Features |
CF®543 | 541/560 nm | 5 mL | 20966 | CF®543 Features |
CF®594 | 593/614 nm | 5 mL | 20951 | CF®594 Features |
CF®640R | 642/662 nm | 5 mL | 20962 | CF®640R Features |
See our other Drop-n-Stain™ CF® Dye Secondary Antibodies
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CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.
Storage and Handling
Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.
Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:
CF® Dye and biotin conjugates: add 0.5 mL dH2O
HRP or DNP conjugates: add 1 mL dH2O
Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.
Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.
Note: Storage of the antibody for more than a day at final working dilution is not recommended.
References
Download a list of CF® dye references.