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CD3e Monoclonal Mouse Antibody (B-B12)

Reacts with five invariable CD3 chains (designated as and ) with molecular weight ranging from 16-28 kDa.

Product Attributes

Antibody number

1052

Reactivity (target)

CD3e

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

B-B12

Isotype

IgG1, kappa

Molecular weight

20 kDa

Synonyms

CD3E, CD3 epsilon, CD3 TCR complex, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex, CD 3E, T-cell surface antigen T3/Leu-4 epsilon chain

Human gene symbol

CD3E

Entrez gene ID

916

SwissProt

P07766

Unigene

3003

Immunogen

Human T cell leukemia cells

Cellular localization

Cytoplasmic, Membrane/cell surface

Species reactivity

Human

Applications

Immunofluorescence, Flow cytometry

Application notes

Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Immunofluorescence 1-2 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

Jurkat cells. Tonsil and lymph node

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Immunology

Cell/tissue expression

Lymphocytes, T-cells

Tumor expression

Leukemia/lymphoma

Conjugation
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Product Description

Reacts with five invariable CD3 chains (designated as and ) with molecular weight ranging from 16-28 kDa. CD3 is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. The CD3 complex is closely associated at the lymphocyte cell surface with T cell antigen receptor (TCR) and is involved in transducing antigen-recognition signals into cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 1052, Anti-CD3e (B-B12) The prefix indicates conjugation, followed by the antibody number and size suffix.

Antibody # prefixConjugationEx/EmConcentrationStorage Buffer
BNC04CF®405S404/431 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC05CF®405M408/452 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC88CF®488A490/515 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC43CF®543541/560 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC55CF®555555/565 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC68CF®568562/583 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC94CF®594593/614 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC40CF®640R642/662 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC47CF®647650/665 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC61CF®660R663/682 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC80CF®680681/698 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC81CF®680R680/701 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC70CF®770770/797 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCRR-PE (PE)496, 546, 565/578 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCAAPC650/660 nnm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCPPerCP482/677 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCBBiotinN/A0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCAPAlkaline PhosphataseN/A0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCHHorseradish PeroxidaseN/A0.1 mg/mL PBS, 0.05% BSA, no azide
BNUBPurified, with BSA N/A0.2 mg/mL PBS, 0.05% BSA, 0.05% azide
BNUMPurified, BSA-freeN/A1 mg/mL PBS, no BSA, no azide

References

Kishimoto T. et al., eds. Leukocyte Typing VI, p44-48 and p1111, Garland Publishing, Inc, New York and London, 1997.

Meuer SC et al. Nature 1983, 303(5920):808-810.
Reinherz et al., Cell 30, 715, (1982).

Borst et al., J. Biol. Chem. 258, 5135, (1983).

Van den Elsen et al., Nature 312, (1984).

Furley et al., Cell 46, 75, (1986).

Gold et al., Nature 321, 4, (1986).

Oettgen and Terhorst, Hum. Immunol. 18, 187, (1987).

Clevers et al., Ann. Rev. Immunol. 6, 629, (1988).

 

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