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NucView® 530 Caspase-3 Substrate, 1 mM in DMSO

Orange fluorescent caspase-3/7 substrate for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or live cell imaging.

Product Attributes

Apoptosis/viability marker

Caspase

For live or fixed cells

For live/intact cells

Assay type/options

Long term staining (24-72h), Endpoint assay, No-wash staining, Real-time imaging, Homogenous assay

Detection method/readout

Fluorescence microscopy, Live cell imaging, Flow cytometry

Substrate specificity

Caspases

Colors

Orange

Fixation options

Fix after staining (formaldehyde), Permeabilize after staining

Excitation/Emission

528/563 nm (with DNA)

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Trial size (10 uL)
100 uL
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Product Description

NucView® 530 Caspase-3 Substrate detects apoptosis in intact cells based on caspase-3/7 activity using confocal microscopy, flow cytometry, or live cell imaging.

  • NucView® 530 dye has excitation/emission maxima of 528/563 nm (with DNA)
  • Rapid, no-wash, endpoint or real-time assays
  • Non-toxic, allowing multi-day experiments to be performed
  • For flow cytometry, microscopy or live cell imaging systems
  • Dual detection of caspase activity and nuclear morphology
  • Formaldehyde fixable

NucView® substrates consist of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with fluorescence. Thus, NucView caspase-3 substrates are bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, NucView® caspase-3 substrates detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. Staining is compatible with subsequent fixation and permeabilization for immunostaining.

NucView® 530 Caspase-3 Substrate stains apoptotic cell nuclei with orange fluorescence (Ex/Em 528/563 nm), for detection in the Cy®3 channel by fluorescence microscopy or the PE channel by flow cytometry. NucView® 530 can be used for multi-color imaging with blue, green, or far-red fluorescent probes. Note that when excited by the 488 nm laser line, NucView® 530 also fluoresces in the FITC channel, and therefore cannot be analyzed together with green probes by flow cytometry.

NucView® 530 is also available as 1 mM in PBS (catalog number 10408) for use with DMSO-sensitive cell types.

We also offer blue fluorogenic NucView® 405 Caspase-3 Substrate, and green fluorogenic NucView® 488 Caspase-3 Substrate and kits.

Excitation and emission spectra of NucView 530 dye with dsDNA.
Figure 1. Excitation and emission spectra of NucView® 530 dye with dsDNA.
NucView® enzyme substrate technology is covered by U.S. patents. Cy dye is a registered trademark of GE Healthcare.
 

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