Mix-n-Stain™ CF™ Dye Antibody Labeling Kits
Tolerate BSA, Gelatin, Ascites Fluid and Tris; 30 Min to Complete; and 100% Yield
Mix-n-Stain™ antibody labeling kits are a revolutionary technology that dramatically simplifies the process of antibody labeling. The kits enable a researcher to covalently label 1 – 100 ug of an antibody with one of our 20 super bright and photostable CF™ dyes or biotin in only 30 minutes and ~100% yield. The prepared conjugate is as good as the best pre-labeled antibody you can buy from some of the leading suppliers.
Each Mix-n-Stain kit comes with everything you need to perform the conjugation. Simply mix your antibody with a vial of pre-measured CF™ dye or biotin in the buffer provided, a step taking less than 30 seconds of hands-on time. After waiting for another 30 minutes, without a separation step, you will have an optimally labeled antibody conjugate ready to be used in any immunofluorescence staining experiment.
One breakthrough feature of the kits is that antibody labeling can be conducted in the presence of virtually any amount of BSA, gelatin or non-antibody proteins in ascites fluid when the new, modified protocol is used. Azide and low level of Tris are also tolerated. As a result, the kits are fully compatible with most commercial antibodies supplied in various formulations without a pre-treatment step prior to the conjugation process.
Another major advantage of the labeling kits is our superior CF™ dyes, many of which are brighter and more photostable than Alexa Fluor® dyes and other similar commercial dyes. With a collection of 20 CF™ dyes spanning the entire visible and near-infrared spectra, you have unprecedented freedom to label any commercial primary antibody with a color of your choice or biotin label, making multi-color detection simple and affordable.
Frequently Asked Questions and Answers:
Q: Is the labeling chemical or physical?
A: The labeling is chemical, which means the dye is covalently attached to the antibody. Covalent labeling ensures no dye transfer or diffusion in multi-color detection employing multiple antibodies labeled with different CF Mix-n-Stain kits.
Q: What are the advantages of Mix-n-Stain kits over Invitrogen’s Zenon® antibody labeling kits?
A: There are four major advantages: 1) Unlike Zenon, Mix-n-Stain labeling covalently attaches the dye to the antibody, eliminating dye transfer or diffusion between antibodies during multi-color staining; 2) Mix-n-Stain conjugates are stable for at least 6 months in storage buffer, whereas Zenon complexes must be used within 30 minutes; 3) Mix-n-Stain conjugates are less bulky because the dyes are directly linked to the antibody, unlike Zenon conjugates which use antibody fragments; 4) No post-staining fixation is required with Mix-n-Stain; 5) Unlike Zenon, Mix-n-Stain labeling is not species-specific. More >>
Table A. Mix-n-Stain™ product list
|Unit Size||Unit Price||Dye Flyer||Protocol||Mix-n-Stain Flyer|
|**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.|