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Goat Anti-Mouse IgG, F(ab’)2 Fragment, Fc Gamma-Specific, Highly Cross-Adsorbed, CF®640R Conjugate

Highly cross-adsorbed F(ab’)2 fragment goat anti-mouse IgG, Fcγ fragment specific secondary antibody labeled with the far-red fluorescent dye CF®640R.

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500 ug
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Product Description

This product is prepared by labeling high quality F(ab’)2 fragment goat anti-mouse IgG, Fcγ fragment specific, with the far-red fluorescent dye CF®640R. The antibody has been adsorbed against human, bovine and horse serum to minimize cross-reactivity. CF®640R is a better alternative to Cy®5, Alexa Fluor® 647 and other spectrally similar dyes.  Learn more about CF®640R.

Advantages of CF®640R

  • A novel rhodamine-based dye spectrally very similar to Cy®5 and Alexa Fluor® 647
  • Much brighter than Cy®5 and at least as bright as Alexa Fluor® 647 when excited at 633 or 635 nm
  • Exceptional photostability: far more photostable than Cy®5 and Alexa Fluor® 647
  • More photostable than Atto 647N, a dye that has been extensively used in single molecule-based imaging
  • Extremely water-soluble and pH-insensitive.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.
Alexa Fluor is a registered trademark of Thermo Fisher Scientific. Cy Dye is a registered trademark of Cytiva.

References

Download a list of curated CF® Dye references.

 

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