For CF® dye, cyanine dye, FITC, biotin, DNP, and dig kits, see the Mix-n-Stain™ Kit Compatibility and Protocol Selection Flowchart, below. We also recommend downloading the updated Product Protocol and completing the pre-labeling checklist to find the right protocol and kit size to use for each antibody you wish to label. Learn more about CF® dyes on our CF® Dye Technology Page, or download the CF® Dye Brochure.
CF® dyes are highly water soluble, small organic dyes designed by scientists at Biotium for labeling proteins and nucleic acids. With a series of over 20 colors (and growing), many of our CF® dyes are brighter and more photostable than competing dyes. For more information please see the product flyers for individual CF® dyes, the CF® Dye Selection Guide, and our CF® Dye FAQs.
Reaction takes only 30 minutes, with minimal hands-on time
Wide selection of our next-gen CF® dyes and other dyes
One labeling reaction of 1 mg IgG per kit
Vial of pre-measured lyophilized dye
Mix-n-Stain™ Maxi Antibody Labeling Kits contain everything you need to rapidly label 1 mg of IgG with one of our next-generation CF® dyes or other label. Simply mix your antibody with the reaction buffer and optimally formulated dye provided, followed by a 30 minute incubation and addition of the provided stop buffer. Any remaining free dye or other label is no longer reactive at the end of the labeling, so the conjugate is ready for staining without further purification. Note the labeling protocol and buffer requirements for the Maxi kits differ from our original micro-scale Mix-n-Stain™ kits, see the Reaction Compatibility Chart below.
After the reaction, the antibody will be labeled with an average of 4-6 dye molecules per antibody molecule. Mix-n-Stain™ labeling is covalent, so labeled antibodies can be used for multiplex staining without transfer of dyes/labels between antibodies. These kits are optimized for a single labeling reaction with 1 mg of IgG; the labeling conditions may cause IgM antibodies to denature.
Our 1 mg scale Mix-n-Stain™ Maxi Kits are available with a selection of our bright and photostable CF® dyes, as well as Cyanine 555 or Cyanine 647 (structurally identical to Cy®3 or Cy®5), or fluorescein/FITC. See Ordering Information below.
Choose the right kit for your application
Mix-n-Stain™ kits are not designed to be used for multiple labeling reactions. For smaller-scale conjugation, see our original Mix‑n‑Stain™ CF® Dye Antibody Labeling Kits; original Mix-n-Stain™ kits also have greater tolerance for antibody buffer additives, and provide special protocols for labeling antibodies in buffer with BSA or in ascites fluid.
Mix-n-Stain™ kits can be used to label other proteins, however, the degree of labeling (DOL, or number of dye molecules per antibody) may not be optimal, and the kits are not designed to allow for DOL measurement after labeling. For performing reactions to optimize DOL for your antibody or application, we recommend using our CF® Dye SE Protein Labeling Kits. The kits include everything you need to perform controlled conjugation reactions and post-labeling purification, as well as detailed instructions and correction factors for determining DOL for CF® dyes.
We offer a variety of kits for labeling antibodies and other proteins:
Paraformaldehyde, 4% in PBS is a ready-to-use fixation solution for cells or tissues. It is electron microscopy-grade paraformaldehyde dissolved in pH 7.4 PBS, and is stabilized by packaging under argon with no methanol added.
The TrueBlack® Background Suppressor System is designed for optimal blocking of non-specific antibody binding as well as direct interaction of fluorescent dyes on antibodies with cells or tissue sections.
TrueBlack® Lipofuscin Autofluorescence Quencher, 20X in DMF, is a new reagent for quenching lipofuscin autofluorescence in tissue sections for immunofluorescence staining. It can also help reduce autofluorescence from other sources like extracellular matrix.
We offer centrifugal ultrafiltration devices with two molecular weight cut-off options: MWCO=10 kD (10K) and MWCO=3 kD (3K) for simple spin-column purification of proteins or other molecules by size separation.