PCR & DNA Amplification

DAPI is a popular blue fluorescent DNA dye. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement.

A high purity grade, red fluorescent nucleic acid dye for detecting nucleic acids in solution or for selectively staining dead cells with damaged plasma membranes.

6X DNA loading buffer that includes ultra-sensitive, non-toxic GelRed® dye.

Stand-alone total RNA standard provided with the AccuBlue® Broad Range RNA Quantitation Kit (31073).

A 10 mg/mL RNase solution, DNase-free. Useful for removing RNA from plasmid or genomic DNA preparation, recombinant protein preparations, or for use in ribonuclease protection assays.

A decontamination solution that effectively removes RNases and other contamination from surfaces and instruments for RNA research.

Fluorescent assay for sensitive detection of RNase activity in liquid samples. Ideal for quality control workflows or detecting RNase contamination.

7-AAD (7-aminoactinomycin D) is a membrane-impermeant fluorescent DNA binding dye that is useful for live/dead discrimination and cell cycle profiling by flow cytometry.

A fluorescent dsDNA quantitation kit with a wide linear range of 0.03-250 ng DNA. Designed for use in a fluorescence microplate reader.

Acridine orange (AO) stains dsDNA green (525 nm) and RNA or single stranded DNA red (650 nm). The dye is membrane-permeant and its nucleic acid binding property has been used for cell-cycle studies.

Actinomycin D is a nonfluorescent GC-selective intercalator. Similar to 7-AAD (cat. no. 40038), actinomycin D has also been used for chromosome banding studies.

ARP (N-(Aminooxyacetyl)-N’-(D-biotinoyl)hydrazine, trifluoroacetic acid salt) reacts with the exposed aldehyde group formed at abasic sites in damaged DNA, allowing the DNA to be labeled with biotin groups (1,2). The labeled DNA can then be quantitated with fluorescent or enzyme-conjugated streptavidin complexes.

DiSC₂(5) (3,3′-Diethylthiadicarbocyanine, iodide) binds to DNA-DNA or PNA-DNA duplexes with a large blue-shift of the absorption spectrum. Binding of the dye to PNA-DNA hybrid, in particular, blue-shifts the dye absorption spectrum by as much as 115 nm, offering a visual method to detect nucleic acid hybridization (1,2).

DODC (DiOC₂(5)) has been reported to bind to triplex DNA as well as dimeric hairpin quadruplex DNA (1). Binding of the dye to triplex DNA quenches the fluorescence of the dye and red-shifts the dye absorption peak.

Ethidium bromide (EtBr) is a widely used nucleic acid gel stain although it is also a known potent mutagen. EtBr is an intercalating DNA-binding dye with little sequence preference.

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains.