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Wheat Germ Agglutinin (WGA) Conjugates

This product is wheat germ agglutinin (WGA) labeled with Biotium’s CF® Dyes or HRP. WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine.

Product Attributes

Cellular localization

Membrane/cell surface

For live or fixed cells

For fixed cells, For live/intact cells

Cell permeability

Membrane impermeant

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Colors

Blue, Green, Orange, Red, Far-red, Near-infrared

Conjugation
Size
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1 mg
5 x 1 mg
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Product Description

This product is wheat germ agglutinin (WGA) labeled with Biotium’s CF® Dyes or HRP. WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine. CF® Dye WGA conjugates can be used to stain glycoproteins that have these sugar moieties. WGA is commonly used to label glycoproteins for imaging of the plasma membrane in live or fixed cells, for staining of tissue sections, or for western blotting. WGA also is useful as a retrograde or anterograde neuronal tracer. Staining with WGA conjugates may be tissue- and cell-type dependent.

WGA can be used as a gram stain to fluorescently label gram+ bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae. See our Cellular Stains Table for more information on how our dyes stain various organisms.

WGA HRP conjugate can be used for Western Blot, ELISA, immunohistochemistry and other standard immunoassay applications.

ProductConjugationEx/EmSizeCatalog No.Purchase
HRP WGAHRPN/A1 mg29073Purchase 29073
CF®350 WGACF®350347/448 nm1 mg29021-1Purchase 29021-1
5 mg29021Purchase 29021
CF®405S WGACF®405S404/431 nm1 mg29027-1Purchase 29027-1
5 mg29027Purchase 29027
CF®405M WGACF®405M408/452 nm1 mg29028-1Purchase 29028-1
5 mg29028Purchase 29028
CF®488A WGACF®488A490/515 nm1 mg29022-1Purchase 29022-1
5 mg29022Purchase 29022
CF®532 WGACF®532527/558 nm1 mg29064-1Purchase 29064-1
5 mg29064Purchase 29064
CF®555 WGACF®555555/565 nm5 mg29076Purchase 29076
1 mg29076-1Purchase 29076-1
CF®568 WGACF®568562/583 nm5 mg29077Purchase 29077
1 mg29077-1Purchase 29077-1
CF®594 WGACF®594593/614 nm1 mg29023-1Purchase 29023-1
5 mg29023Purchase 29023
CF®633 WGACF®633630/650 nm1 mg29024-1Purchase 29024-1
5 mg29024Purchase 29024
CF®640R WGACF®640R642/662 nm1 mg29026-1Purchase 29026-1
5 mg29026Purchase 29026
CF®680 WGACF®680681/698 nm1 mg29029-1Purchase 29029-1
5 mg29029Purchase 29029
CF®680R WGA CF®680R680/701 nm1 mg29025-1Purchase 29025-1
5 mg29025Purchase 29025
CF®770 WGACF®770770/797 nm1 mg29059-1Purchase 29059-1
5 mg29059Purchase 29059

References

CF® Dye WGA in bacteria
Ruger M., et al. (2014). Species-specific viability analysis of Pseudomonas aeruginosa, Burkholderia cepacia and Staphylococcus aureus in mixed culture by flow cytometry. BMC Microbiol. Mar 7;14:56.

CF® Dye WGA in fungi
Falter C. & Voigt C. (2017) The Eurotiomycete Apinisia graminicola as the causal agent of a leaf spot disease on the energy crop Miscanthus x giganteus in Northern Germany. Eur J Plant Pathol 149, 797–806.

Yang J, et al. (2017) Transcriptomic profiling of Alternaria longipes invasion in tobacco reveals pathogenesis regulated by AlHK1, a group III histidine kinase. Sci Rep. 2017 Nov 22;7(1):16083.

CF® Dye WGA in cells & tissues
Lasher RA., et al. (2012) Electrical stimulation directs engineered cardiac tissue to an age-matched native phenotype. J Tissue Eng. 2012;3(1):2041731412455354.

Adolfsson K., et al. (2013) Ingestion of gallium phosphide nanowires has no adverse effect on Drosophila tissue function. Nanotechnology (24), 28.

Ho AK., et al. (2016) The topology, structure and PE interaction of LITAF underpin a Charcot-Marie-Tooth disease type 1C. BMC Biol. Dec 7;14(1):109.

Mohammadpour R., et al. (2016) Comparative Endocytosis Mechanisms and Anticancer Effect of HPMA Copolymer- and PAMAM Dendrimer-MTCP Conjugates for Photodynamic Therapy. Macromol. Biosci. Apr;17(4).

Reed AP., et al. (2016) Dominant-Negative Effect of a Missense Variant in the TASK-2 (KCNK5) K+ Channel Associated with Balkan Endemic Nephropathy. PLoS One. May 26;11(5):e0156456.

Kolahi KS., et al. (2017) Cytotrophoblast, Not Syncytiotrophoblast, Dominates Glycolysis and Oxidative Phosphorylation in Human Term Placenta. Sci Rep. Feb 23;7:42941.

Loh LN., et al. (2017) Dissecting Bacterial Cell Wall Entry and Signaling in Eukaryotic Cells: an Actin-Dependent Pathway Parallels Platelet-Activating Factor Receptor-Mediated Endocytosis. MBio. Jan 3;8(1).

Kumaran GK & Hanukoglu I. (2019) Identification and classification of epithelial cells in nephron segments by actin cytoskeleton patterns. FEBS J. Oct 12.

Yu Y., et al. (2019) Expression, Localization and Functional Activity of the Major Na+/H+ Exchange Isoforms Expressed in the Intestinal Cell Line Caco-2BBe. Cell Physiol Biochem. 2019;52(5):1017-1038.

 

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