This product is wheat germ agglutinin (WGA) labeled with Biotium’s CF® Dyes or HRP. WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine. CF® Dye WGA conjugates can be used to stain glycoproteins that have these sugar moieties. WGA is commonly used to label glycoproteins for imaging of the plasma membrane in live or fixed cells, for staining of tissue sections, or for western blotting. WGA also is useful as a retrograde or anterograde neuronal tracer. Staining with WGA conjugates may be tissue- and cell-type dependent.
WGA can be used as a gram stain to fluorescently label gram+ bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae. See our Cellular Stains Table for more information on how our dyes stain various organisms.
WGA HRP conjugate can be used for Western Blot, ELISA, immunohistochemistry and other standard immunoassay applications.
CF® Dye WGA in bacteria
Ruger M., et al. (2014). Species-specific viability analysis of Pseudomonas aeruginosa, Burkholderia cepacia and Staphylococcus aureus in mixed culture by flow cytometry. BMC Microbiol. Mar 7;14:56.
CF® Dye WGA in fungi
Falter C. & Voigt C. (2017) The Eurotiomycete Apinisia graminicola as the causal agent of a leaf spot disease on the energy crop Miscanthus x giganteus in Northern Germany. Eur J Plant Pathol 149, 797–806.
Yang J, et al. (2017) Transcriptomic profiling of Alternaria longipes invasion in tobacco reveals pathogenesis regulated by AlHK1, a group III histidine kinase. Sci Rep. 2017 Nov 22;7(1):16083.
CF® Dye WGA in cells & tissues
Lasher RA., et al. (2012) Electrical stimulation directs engineered cardiac tissue to an age-matched native phenotype. J Tissue Eng. 2012;3(1):2041731412455354.
Adolfsson K., et al. (2013) Ingestion of gallium phosphide nanowires has no adverse effect on Drosophila tissue function. Nanotechnology (24), 28.
Ho AK., et al. (2016) The topology, structure and PE interaction of LITAF underpin a Charcot-Marie-Tooth disease type 1C. BMC Biol. Dec 7;14(1):109.
Mohammadpour R., et al. (2016) Comparative Endocytosis Mechanisms and Anticancer Effect of HPMA Copolymer- and PAMAM Dendrimer-MTCP Conjugates for Photodynamic Therapy. Macromol. Biosci. Apr;17(4).
Reed AP., et al. (2016) Dominant-Negative Effect of a Missense Variant in the TASK-2 (KCNK5) K+ Channel Associated with Balkan Endemic Nephropathy. PLoS One. May 26;11(5):e0156456.
Kolahi KS., et al. (2017) Cytotrophoblast, Not Syncytiotrophoblast, Dominates Glycolysis and Oxidative Phosphorylation in Human Term Placenta. Sci Rep. Feb 23;7:42941.
Loh LN., et al. (2017) Dissecting Bacterial Cell Wall Entry and Signaling in Eukaryotic Cells: an Actin-Dependent Pathway Parallels Platelet-Activating Factor Receptor-Mediated Endocytosis. MBio. Jan 3;8(1).
Kumaran GK & Hanukoglu I. (2019) Identification and classification of epithelial cells in nephron segments by actin cytoskeleton patterns. FEBS J. Oct 12.
Yu Y., et al. (2019) Expression, Localization and Functional Activity of the Major Na+/H+ Exchange Isoforms Expressed in the Intestinal Cell Line Caco-2BBe. Cell Physiol Biochem. 2019;52(5):1017-1038.