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Bovine Anti-Goat IgG (H+L), Highly Cross-Adsorbed

Highly cross-adsorbed bovine anti-goat IgG (H+L) secondary antibody labeled with our superior CF® dyes.

Product Attributes

Clonality

Polyclonal

Antibody type

Secondary

Species reactivity

Goat

Host species

Bovine

Reactivity (target)

Goat IgG

Cross adsorption

Bovine, Chicken, Guinea pig, Horse, Human, Mouse, Rabbit, Rat, Syrian hamster

Applications

Immunofluorescence, Immunohistology (formalin), Immunohistology (frozen), Western

Secondary antibody formats:
Liquid format:

  • CF® dye and biotin conjugates: 2 mg/mL in PBS, 50% glycerol, 2 mg/ml BSA, 0.05% sodium azide
  • HRP conjugates: 1 mg/mL in PBS, 50% glycerol, 15 mg/ml BSA
  • Alkaline phosphatase conjugates: 1 mg/mL in PBS, 40% glycerol, 5 mg/ml BSA, 0.05% sodium azide
  • R-PE and APC conjugates: 0.5 mg/mL in PBS, 2 mg/ml
    BSA, 0.05% sodium azide

Lyophilized format (after reconstitution):

  • CF® dye and biotin conjugates: 2 mg/mL in PBS, 15 mg/mL BSA, 20 mg/mL trehalose
  • HRP conjugates: 1 mg/mL PBS, 10 mg/mL trehalose
Conjugation
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Size
Catalog #
price
Qty
50 uL
0.5 mL
1 mg
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Product Description

This is a highly cross-adsorbed bovine anti-goat IgG (H+L) secondary antibody labeled with our bright and photostable CF® dyes. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum proteins.

  • Highly cross-adsorbed for specific staining with minimal background
  • Minimal cross-reactivity with milk or BSA blocking solutions
  • Available in 6 bright and photostable CF® dyes
  • Suitable for western, immunofluorescence, and immunohistology

Bovine host minimizes anti-goat (IgG) cross-reactivity with bovine IgG

Bovine serum albumin (BSA) and dry milk are used extensively for blocking non-specific binding of antibodies and for stabilizing antibodies and other proteins during freeze drying and in dilute solutions. However dry milk and most commercial sources of BSA contain bovine IgG which interferes with the use of anti-goat (IgG) secondary antibodies due to the close phylogenetic relationship between cows and goats. This causes non-specific background which may lead to false results and needless repetition of experiments. This antibody is created by immunizing a cow host with goat IgG which helps minimize cross-reactivity with bovine IgG and therefore reduce background from BSA containing solutions.

Bovine Anti-Goat IgG (H+L), Highly Cross-Adsorbed

ConjugationEx/EmSizeCatalog No.
CF®488A490/515 nm50 uL (100 ug)20293-1
0.5 mL (1 mg)20293
1 mg (lyophilized)20293-1mg
CF®543541/560 nm50 uL (100 ug)20313-1
0.5 mL (1 mg)20313
1 mg (lyophilized)20313-1mg
CF®568562/583 nm50 uL (100 ug)20294-1
0.5 mL (1 mg)20294
1 mg (lyophilized)20294-1mg
CF®594593/614 nm50 uL (100 ug)20295-1
0.5 mL (1 mg)20295
1 mg (lyophilized)20295-1mg
CF®633630/650 nm50 uL (100 ug)20296-1
0.5 mL (1 mg)20296
1 mg (lyophilized)20296-1mg
CF®640R642/662 nm50 uL (100 ug)20297-1
0.5 mL (1 mg)20297
1 mg (lyophilized)20297-1mg

View our full selection of bright and specific Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

CF is a registered trademark of Biotium, Inc.

References

Download a list of CF® dye references.

 

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