This CF™620R tyramide conjugate is used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. A tyramide conjugate is incubated in the presence of HRP (often bound to a secondary antibody), which catalyzes the binding of the tyramide (and associated label) to adjacent tyrosines for enhanced fluorescent signal.
CF™620R is highly fluorescent and extremely photostable. With its absorption and emission maxima centered at 617 and 639 nm, respectively, the dye may be used as an excellent energy acceptor in fluorescence resonance energy transfer (FRET), or used in multi-color detection applications where the excitation and emission windows can be matched with the spectral profiles of the dye for maximal fluorescence collection. The exceptional water solubility of the dye facilitates bioconjugation in aqueous media and better retains the biological specificity of the conjugates.