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Cholera Toxin Subunit B CF® Dye Conjugates

Binds GM1 ganglioside in lipid rafts and is used as a retrograde neuronal tracer for live imaging or on fixed cells.

Product Attributes

Probe cellular localization

Membrane/cell surface

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

Real-time imaging

Detection method/readout

Fluorescence microscopy, Flow cytometry

Cell permeability

Membrane impermeant

Fixation options

Fix after staining (formaldehyde), Fix before staining (formaldehyde)

Toxin

Cholera toxin

Colors

Blue, Green, Orange, Red, Far-red, Near-infrared

Conjugation
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Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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100 ug
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Product Description

Cholera toxin B subunit is used for tract-tracing in neurological research, targeting GM1 ganglioside binding and retrograde transport.

  • Choice of 11 bright & photostable CF® Dyes from green to near-infrared
  • Fluorescent lipid raft markers and retrograde neuronal tracers for live imaging or on fixed cells

Cholera toxin is the symptom-causing toxin produced by the bacteria Vibrio cholerae during cholera infection. The toxin is composed of two subunits, A and B. Subunit A is the toxic enzymatic subunit present in one copy per toxin. Cholera toxin subunit B (CT-B) is the receptor binding subunit that is found as a pentamer in each toxin and is relatively non-toxic, making it useful for cell biological studies. CT-B has been used as a neuronal tracer and has also been shown to bind to GM1 gangliosides that are found in lipid rafts on the surface of mammalian cells. Therefore, fluorescently labeled conjugates of CT-B have been used as lipid raft markers and endocytic tracers for live imaging or on fixed cells.

Superior CF® Dyes

Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

CF® Dye Cholera Toxin Conjugates

ConjugationEx/EmSizeCatalog No.Dye Features
CF®405M408/452 nm100 ug00068CF®405M Features
CF®488A490/515 nm100 ug00070CF®488A Features
CF®532527/558 nm100 ug00074CF®532 Features
CF®543541/560 nm100 ug00075CF®543 Features
CF®568562/583 nm100 ug00071CF®568 Features
CF®594593/614 nm100 ug00072CF®594 Features
CF®633630/650 nm100 ug00077CF®633 Features
CF®640R642/662 nm100 ug00073CF®640R Features
CF®647650/665 nm100 ug00069CF®647 Features
CF®660R663/682 nm100 ug00078CF®660R Features
CF®680R680/701 nm100 ug00079CF®680R Features
CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific.

References

CF®568 Cholera Toxin (00071)
Nature (2016) 529, 408–412. https://doi.org/10.1038/nature16516
Nature (2017) 546, 492–497. https://doi.org/10.1038/nature22818

Download a list of CF® dye references.

 

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