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DAPI

DAPI is a popular blue fluorescent DNA dye. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement.

Product Attributes

CAS number

28718-90-3

Cellular localization

Nucleus

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

DNA content/cell cycle (flow cytometry), Long term staining (24-72h), No-wash staining, Real-time imaging

Cell permeability

Membrane permeant

Apoptosis/viability marker

All cell stain

Colors

Blue

Excitation/Emission

358/461 nm (with DNA)

Size
Catalog #
price
Qty
10 mg
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Protocols
SDS

Product Description

DAPI (4′,6-Diamidino-2-Phenylindole, dihydrochloride) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions. Also see DAPI dilactate (catalog no. 40009), a more water soluble form of the dye.

DAPI can be used to stain live bacteria (gram-positive and gram-negative) but in live yeast the staining is weak and not nuclear. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Product Features:

  • Classic blue nuclear counterstain for fixed cells
  • Can be used at higher concentrations to stain live cells
  • λExEm (with DNA) = 358/461 nm
  • Off-white solid soluble in H2O
  • Store at 4°C and protect from light, especially in solution
  • C16H17Cl2N5
  • MW = 350.25
  • [28718-90-3]

 

Learn more about DAPI and our novel nuclear stains. We also offer antifade mounting media containing DAPI, in both hardset and wetset formulations.

DAPI Products

Product

Unit Size

Catalog Number

DAPI10 mg40011
DAPI in H2O, 10 mg/mL1 mL40043
DAPI, dilactate10 mg40009

References

1. Biotechnic Histochem 70, 220 (1995).

 

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