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Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed

This product is prepared by labeling highly cross-adsorbed donkey anti-sheep IgG (H+L) with a selection of our bright and photostable CF™ dyes. To minimize cross-reactivity, the antibody has been adsorbed against chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum.

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Secondary antibody formats:
Liquid format:

  • CF™ dye and biotin conjugates: 2 mg/mL in PBS, 50% glycerol, 2 mg/ml BSA, 0.05% sodium azide
  • HRP conjugates: 1 mg/mL in PBS, 50% glycerol, 15 mg/ml BSA
  • Alkaline phosphatase conjugates: 1 mg/mL in PBS, 40% glycerol, 5 mg/ml BSA, 0.05% sodium azide
  • R-PE and APC conjugates: 0.5 mg/mL in PBS, 2 mg/ml
    BSA, 0.05% sodium azide

Lyophilized format (after reconstitution):

  • CF™ dye and biotin conjugates: 2 mg/mL in PBS, 15 mg/mL BSA, 20 mg/mL trehalose
  • HRP conjugates: 1 mg/mL PBS, 10 mg/mL trehalose
Catalog #

20024, 20095-1mg, 20134-1, 20134-1mg, 20148-1, 20148-1mg, 20156-1, 20156-1mg, 20234-1, 20234-1mg, 20284-1, 20284-1mg, 20322-1, 20095-1, 20083-1mg, 20083-1, 20062, 20083, 20095, 20134, 20148, 20156, 20234, 20284, 20322, 20024-1, 20024-1mg, 20062-1, - 20322-1mgView allHide

Size

50 uL, 0.25 mL, 0.5 mL, 1 mg

Conjugation

CF350, CF488A, CF543, CF555, CF568, CF594, CF633, CF640R, CF647, CF680

Antibody type

Secondary

Species reactivity

Sheep

Host species

Donkey

Reactivity (target)

Sheep IgG

Cross adsorption

Chicken, Syrian hamster, Rat, Rabbit, Mouse, Human, Horse, Guinea pig

Applications

Immunofluorescence, Immunohistology (formalin), Western, Immunohistology (frozen)

Conjugation
Size
Catalog #
price
Qty
50 uL
0.25 mL
0.5 mL
1 mg
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Product Description

This product is prepared by labeling highly cross-adsorbed donkey anti-sheep IgG (H+L) with a selection of fluorescent CF™ dyes. To minimize cross-reactivity, the antibody has been adsorbed against chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum.
CF™ dyes offer exceptional brightness and photostability. See the CF™ Dye Selection Guide under Downloads for more information.

Note: Conjugates of blue fluorescent dyes like CF™350 are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed: Product & SKU Summary

ProductConjugationEx/EmSizeSKUPurchase
CF350 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF350347/448 nm0.5 mL20148Purchase 20148
CF350 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF350347/448 nm50 μL20148-1Purchase 20148-1
CF350 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF350347/448 nm1 mg20148-1mgPurchase 20148-1mg
CF488A Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF488A490/515 nm0.5 mL20024Purchase 20024
CF488A Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF488A490/515 nm50 μL20024-1Purchase 20024-1
CF488A Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF488A490/515 nm1 mg20024-1mgPurchase 20024-1mg
CF543 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF543541/560 nm0.5 mL20322Purchase 20322
CF543 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF543541/560 nm50 μL20322-1Purchase 20322-1
CF543 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF543541/560 nm1 mg20322-1mgPurchase 20322-1mg
CF555 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF555555/565 nm0.5 mL20234Purchase 20234
CF555 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF555555/565 nm50 μL20234-1Purchase 20234-1
CF555 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF555555/565 nm1 mg20234-1mgPurchase 20234-1mg
CF568 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF568562/583 nm0.5 mL20095Purchase 20095
CF568 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF568562/583 nm50 μL20095-1Purchase 20095-1
CF568 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF568562/583 nm1 mg20095-1mgPurchase 20095-1mg
CF594 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF594593/614 nm0.5 mL20156Purchase 20156
CF594 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF594593/614 nm50 μL20156-1Purchase 20156-1
CF594 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF594593/614 nm1 mg20156-1mgPurchase 20156-1mg
CF633 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF633630/650 nm0.5 mL20134Purchase 20134
CF633 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF633630/650 nm50 μL20134-1Purchase 20134-1
CF633 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF633630/650 nm1 mg20134-1mgPurchase 20134-1mg
CF640R Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF640R642/662 nm0.5 mL20083Purchase 20083
CF640R Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF640R642/662 nm50 μL20083-1Purchase 20083-1
CF640R Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF640R642/662 nm1 mg20083-1mgPurchase 20083-1mg
CF647 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF647650/665 nm0.5 mL20284Purchase 20284
CF647 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF647650/665 nm50 μL20284-1Purchase 20284-1
CF647 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF647650/665 nm1 mg20284-1mgPurchase 20284-1mg
CF680 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF680681/698 nm0.25 mL20062Purchase 20062
CF680 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbedCF680681/698 nm50 μL20062-1Purchase 20062-1

References

Download a list of CF dye references.

 

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Support & Faq

Product shelf life, solubility, shipping and storage, and stability

Bioscience kits
The guaranteed shelf life from date of receipt for bioscience kits is listed on the product information sheet. Some kits have an expiration date printed on the kit box label, this is the guaranteed shelf life date calculated from the day that the product shipped from our facility. Kits often are functional for significantly longer than the guaranteed shelf life. If you have an older kit in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the kit still works for your application before processing a large number of samples or precious samples.

Antibodies and other conjugates
The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. If you have an older conjugate in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.

For lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates).

Chemicals, dyes, and gel stains
Biotium guarantees the stability of chemicals, dyes, and gel stains for at least a year from the date you receive the product. However, the majority of these products are highly stable for many years, as long as they are stored as recommended. Storage conditions can be found on the product information sheet or product safety and data sheet, material safety data sheet, and on the product label. Fluorescent compounds should be protected from light for long term storage.

If you have a Biotium compound that has been in storage for longer than one year that you wish to use, we recommend performing a small scale positive control experiment to confirm that the compound still works for your application before processing a large number of samples or precious samples.

Expiration date based on date of manufacture (DOM)
If your institution requires you to document expiration date based on date of manufacture for reagents, please contact techsupport@biotium.com for assistance.

Chemical products with special stability considerations:

Esters

Ester compounds include the following:
• Succinimidyl esters (SE, also known as NHS esters), such as our amine-reactive dyes
• Acetoxymethyl esters (AM esters) such as our membrane-permeable ion indicator dyes
• Diacetate-modified dyes, like ViaFluor™ 405, CFDA, and CFDA-SE cell viability/cell proliferation dyes

Ester dyes are stable in solid form as long as they are protected from light and moisture. Esters are not stable in aqueous solution. Concentrated stock solutions should be prepared in anhydrous DMSO (see Biotium catalog no. 90082). Stock solutions in anhydrous DMSO can be stored desiccated at -20°C for one month or longer. Esters should be diluted in aqueous solution immediately before use. Succinimidyl esters (SE) should be dissolved in a solution that is free of amine-containing compounds like Tris, glycine, or protein, which will react with the SE functional group. AM esters and diacetate compounds should be dissolved in a solution that is free of serum, because serum could contain esterases that would hydrolyze the compound.

A note on CF™ dye succinimidyl ester stability
Succinimidyl esters are generally susceptible to hydrolysis, which can result in lower labeling efficiency. Heavily sulfonated dyes, such as the Alexa Fluor® dyes, DyLight® dyes and IRDyes® are particularly hygroscopic, worsening the hydrolysis problem. For example, the percent of active Alexa Fluor® 488 succinimidyl ester (SE) could be well below 50% by the time of application (according to the manufacturer’s product datasheet). In a number of Alexa Fluor® SE reactive dyes, the SE group is derived from an aromatic carboxylic acid, while in all of Biotium’s CF™ dyes the SE group is prepared from an aliphatic carboxylic acid. This structural difference reduces the susceptibility of CF™ dye SE reactive groups to hydrolysis, resulting in relatively stable reactive dyes with consistently higher labeling efficiency compared to other SE derivatives of other fluorescent dyes.

Maleimides, MTS and thiosulfate dyes
Like the succinimidyl ester dyes, these dyes are also susceptible to hydrolysis, although generally to a much lower degree. Thus, for long term storage, anhydrous DMSO is recommended for making stock solutions.

Other reactive dyes
Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react with the dye’s functional group. See the product information sheets for specific reactive dyes more information.

Coelenterazines and D-luciferin

Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. Propylene glycol also can be used as a solvent to minimize evaporation. If the solvent evaporates, the coelenterazine will still be present in the vial, so note the volume in the vial prior to storage so that you can adjust the solvent volume to correct for evaporation if needed. Prepare working solutions in aqueous buffers immediately before use. Coelenterazines are stable for up to five hours in aqueous solution.

Aquaphile™ coelenterazines are water soluble formulations of coelenterazines. They are stable in solid form when stored as recommended. Aquaphile™ coelenterazines should be dissolved in aqueous solution immediately before use. They are stable for up to five hours in aqueous solution.

Note that coelenterazines are predominantly yellow solids, but may contain dark red or brown flecks. This does not affect product stability or performance. If your coelenterazine is uniformly brown, then it is oxidized and needs to be replaced.

D-luciferin is stable in solid form and as a concentrated stock solution when stored as recommended; it is not stable at dilute working concentrations in aqueous solution. Prepare concentrated D-luciferin stock solutions (typically 1-100 mg/mL) in water, and store in aliquots at -20°C or below for six months or longer. Prepare working solutions immediately before use.

Category: Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView™ Caspase 3 Enzyme Substrates, Luciferase Assays, CF™ Dyes, Product shelf life, solubility, shipping and storage, and stability

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Many of our solid compounds are packaged by lyophilization, in which case they usually do not appear as fluffy powders, but form a film or coating on the sides of the vial. Simply add the appropriate volume of the recommended solvent to the vial to make the desired concentration stock solution, and swirl or gently vortex to mix. Make sure the solvent comes in contact with the inside walls of the vial to fully recover the product.

Category: Product shelf life, solubility, shipping and storage, and stability

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Most of our products are stable at room temperature for many days, but we recommend storage at 4°C or -20°C to prolong shelf life. In the case of many of our aqueous dye solutions, the compounds are very stable at room temperature, but we recommend cold storage to prevent the growth of mold or other microbes over time. Therefore, to save on shipping costs, products with recommended storage at 4°C or -20°C may ship at ambient temperature without affecting product performance. When you receive the product, place it under the recommended storage conditions.

Category: Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView™ Caspase 3 Enzyme Substrates, Luciferase Assays, CF™ Dyes, Product shelf life, solubility, shipping and storage, and stability

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.