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Donkey Anti-Rat IgG (H+L), Highly Cross-Adsorbed

Highly cross-adsorbed donkey anti-rat IgG (H+L) secondary antibody labeled with our superior CF® dyes.

Product Attributes

Clonality

Polyclonal

Antibody type

Secondary

Species reactivity

Rat

Host species

Donkey

Reactivity (target)

Rat IgG

Cross adsorption

Bovine, Chicken, Goat, Guinea pig, Horse, Human, Mouse, Rabbit, Sheep, Syrian hamster

Applications

Immunofluorescence, Immunohistology (formalin), Immunohistology (frozen), Western

Secondary antibody formats:
Liquid format:

  • CF® dye and biotin conjugates: 2 mg/mL in PBS, 50% glycerol, 2 mg/ml BSA, 0.05% sodium azide
  • HRP conjugates: 1 mg/mL in PBS, 50% glycerol, 15 mg/ml BSA
  • Alkaline phosphatase conjugates: 1 mg/mL in PBS, 40% glycerol, 5 mg/ml BSA, 0.05% sodium azide
  • R-PE and APC conjugates: 0.5 mg/mL in PBS, 2 mg/ml
    BSA, 0.05% sodium azide

Lyophilized format (after reconstitution):

  • CF® dye and biotin conjugates: 2 mg/mL in PBS, 15 mg/mL BSA, 20 mg/mL trehalose
  • HRP conjugates: 1 mg/mL PBS, 10 mg/mL trehalose
Conjugation
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Product Description

This is a highly cross-adsorbed donkey anti-rat IgG (H+L) secondary antibody labeled with our bright and photostable CF® dyes. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and sheep serum.

  • Highly cross-adsorbed for specific staining with minimal background
  • Available in 12 bright and photostable CF® dyes
  • Suitable for western, immunofluorescence, and immunohistology in FFPE tissues

Note: Conjugates of blue fluorescent dyes like CF®350 are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Donkey Anti-Rat IgG (H+L), Highly Cross-Adsorbed

ProductConjugationEx/EmSizeCatalog number
CF®350 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®350347/448 nm50 uL20361-50uL
CF®350 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®350347/448 nm0.5 mL20361-500uL
CF®350 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®350347/448 nm1 mg20361-1mg
CF®405S Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®405S404/431 nm50 uL20419-50uL
CF®405S Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®405S404/431 nm0.5 mL20419-500uL
CF®405S Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®405S404/431 nm1 mg20419-1mg
CF®488A Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®488A490/515 nm50 uL20027-1
CF®488A Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®488A490/515 nm0.5 mL20027
CF®488A Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®488A490/515 nm1 mg20027-1mg
CF®543 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®543541/560 nm50 uL20320-1
CF®543 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®543541/560 nm0.5 mL20320
CF®543 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®543541/560 nm1 mg20320-1mg
CF®568 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®568562/583 nm50 uL20092-1
CF®568 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®568562/583 nm0.5 mL20092
CF®568 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®568562/583 nm1 mg20092-1mg
CF®594 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®594593/614 nm50 uL20159-1
CF®594 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®594593/614 nm0.5 mL20159
CF®594 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®594593/614 nm1 mg20159-1mg
CF®633 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®633630/650 nm50 uL20137-1
CF®633 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®633630/650 nm0.5 mL20137
CF®633 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®633630/650 nm1 mg20137-1mg
CF®640R Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®640R642/662 nm50 uL20199-1
CF®640R Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®640R642/662 nm0.5 mL20199
CF®640R Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®640R642/662 nm1 mg20199-1mg
CF®647 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®647650/665 nm50 uL20843-50uL
CF®647 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®647650/665 nm0.5 mL20843-500uL
CF®647 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®647650/665 nm1 mg20843-1mg
CF®660R Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®660R663/682 nm50 uL20390-50uL
CF®660R Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®660R663/682 nm0.5 mL20390-500uL
CF®660R Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®660R663/682 nm1 mg20390-1mg
CF®680 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®680681/698 nm50 uL20417-50uL
CF®680 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®680681/698 nm250 uL20417-250uL
CF®750 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®750755/777 nm50 uL20857-50uL
CF®750 Donkey Anti-Rat IgG (H+L), highly cross-adsorbedCF®750755/777 nm250 uL20857-250uL

View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

CF is a registered trademark of Biotium, Inc.

References

Download a list of CF® dye references.

 

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