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Donkey Anti-Rat IgG (H+L), Highly Cross-Adsorbed

Highly cross-adsorbed donkey anti-rat IgG (H L) secondary antibody labeled with our superior CF® Dyes.

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50 uL
250 uL
500 uL
0.5 mL
1 mg
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Product Description

This is a highly cross-adsorbed donkey anti-rat IgG (H L) secondary antibody labeled with our bright and photostable CF® Dyes. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and sheep serum.

  • Highly cross-adsorbed for specific staining with minimal background
  • Available in 14 bright and photostable CF® Dyes
  • Suitable for western, immunofluorescence, and immunohistology in FFPE tissues

Note: Conjugates of blue fluorescent dyes like CF®350 are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Donkey Anti-Rat IgG (H+L), Highly Cross-Adsorbed

ConjugationEx/EmSizeCatalog No.Dye Features
CF®350347/448 nm50 uL (100 ug)20361-50uLCF®350 Features
0.5 mL (1 mg)20361-500uL
1 mg20361-1mg
CF®405S404/431 nm50 uL (100 ug)20419-50uLCF®405S Features
0.5 mL (1 mg)20419-500uL
1 mg20419-1mg
CF®488A490/515 nm50 uL (100 ug)20027-1CF®488A Features
0.5 mL (1 mg)20027
1 mg20027-1mg
CF®543541/560 nm50 uL (100 ug)20320-1CF®543 Features
0.5 mL (1 mg)20320
1 mg20320-1mg
CF®568562/583 nm50 uL (100 ug)20092-1CF®568 Features
0.5 mL (1 mg)20092
1 mg20092-1mg
CF®583R585/609 nm50 uL (100 ug)20896-50uLCF®583R Features
0.5 mL (1 mg)20896-500uL
CF®594593/614 nm50 uL (100 ug)20159-1CF®594 Features
0.5 mL (1 mg)20159
1 mg20159-1mg
CF®633630/650 nm50 uL (100 ug)20137-1CF®633 Features
0.5 mL (1 mg)20137
1 mg20137-1mg
CF®640R642/662 nm50 uL (100 ug)20199-1CF®640R Features
0.5 mL (1 mg)20199
1 mg20199-1mg
CF®647650/665 nm50 uL (100 ug)20843-50uLCF®647 Features
0.5 mL (1 mg)20843-500uL
1 mg20843-1mg
CF®660R663/682 nm50 uL (100 ug)20390-50uLCF®660R Features
0.5 mL (1 mg)20390-500uL
1 mg20390-1mg
CF®680681/698 nm50 uL (100 ug)20417-50uLCF®680 Features
0.25 mL (500 ug)20417-250uL
CF®740742/767 nm50 uL (100 ug)20989-50uLCF®740 Features
0.25 mL (500 ug)20989-250uL
CF®750755/777 nm50 uL (100 ug)20857-50uLCF®750 Features
0.25 mL (500 ug)20857-250uL

View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of curated CF® Dye references.

 

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