Rabbit Anti-Mouse IgG (H+L), Highly Cross-Adsorbed

Highly cross-adsorbed rabbit anti-mouse IgG (H+L) secondary antibody labeled with our superior CF® dyes.

Product Attributes

Clonality	Polyclonal
Antibody type	Secondary
Species reactivity	Mouse
Host species	Rabbit
Reactivity (target)	Mouse IgG
Cross adsorption	Human
Applications	Immunofluorescence, Immunohistology (formalin), Immunohistology (frozen), Western

Learn more about

Technology

CF® Dyes

Considerations for Immunofluorescence Staining

Secondary Antibody Product Listings

Tech Tips

Protocol: Fluorescent Western Blotting

Protocol: Immunofluorescence Staining of Cells for Microscopy

Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host

Secondary antibody formats:
Liquid format:

CF® dye and biotin conjugates: 2 mg/mL in PBS, 50% glycerol, 2 mg/ml BSA, 0.05% sodium azide
HRP conjugates: 1 mg/mL in PBS, 50% glycerol, 15 mg/ml BSA
Alkaline phosphatase conjugates: 1 mg/mL in PBS, 40% glycerol, 5 mg/ml BSA, 0.05% sodium azide
R-PE and APC conjugates: 0.5 mg/mL in PBS, 2 mg/ml
BSA, 0.05% sodium azide

Lyophilized format (after reconstitution):

CF® dye and biotin conjugates: 2 mg/mL in PBS, 15 mg/mL BSA, 20 mg/mL trehalose
HRP conjugates: 1 mg/mL PBS, 10 mg/mL trehalose

FAQs

Product shipping, storage, shelf life, & solubility

I purchased coelenterazine, but it has red/brown flecks. Should I request a replacement?

Coelenterazines are predominantly yellow solids, but may contain dark red or brown flecks. This does not affect product stability or performance. If your coelenterazine is uniformly brown, then it is oxidized and needs to be replaced.

Category: Luciferase Assays, Product shipping, storage, shelf life, & solubility

View more FAQs

My product arrived at room temperature or thawed, but it’s supposed to be stored at 4°C or -20°C. Is it still ok?

Most of our products are stable at room temperature for many days, but we recommend storage at 4°C or -20°C to prolong shelf life. In the case of many of our aqueous dye solutions, the compounds are very stable at room temperature, but we recommend cold storage to prevent the growth of mold or other microbes over time. Therefore, to save on shipping costs, products with recommended storage at 4°C or -20°C may ship at ambient temperature or with an ice pack. These products may thaw without affecting product performance. When you receive the product, place it under the recommended storage conditions.

Category: CF® Dyes, DNA Quantitation Kits, Luciferase Assays, Mix-n-Stain™ Antibody Labeling Kits, NucView® Caspase-3 Enzyme Substrates, Product shipping, storage, shelf life, & solubility

View more FAQs

My product was accidentally left out at room temperature or exposed to light. Is it ruined?

Most of our products are stable at room temperature for many days, so in all likelihood the product will still work just fine. To be on the safe side, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.

One exception that we are aware of is GelGreen™, which is more sensitive to light exposure than most of our other fluorescent dyes. If GelGreen™ is exposed to ambient light for a prolonged period of time (days to weeks), its color will change from dark orange to brick red. If this occurs, the GelGreen will no longer work for gel staining.

Category: Apoptosis, Necrosis, and Cell Viability Kits, CF® Dyes, DNA Quantitation Kits, GelRed® and GelGreen® Nucleic Acid Gel Stains, Luciferase Assays, Mix-n-Stain™ Antibody Labeling Kits, NucView® Caspase-3 Enzyme Substrates, Product shipping, storage, shelf life, & solubility

View more FAQs

Ask a question

SHARE PRODUCT

Share this product with a colleague or purchasing manager.

Copy Link

Send Email

Conjugation

Don’t see what you’re looking for? Contact us for more conjugation options.

Size

Catalog #

price

Qty

50 uL

0.25 mL

0.5 mL

1 mg

Clear selection

Add to wishlist

ADD TO CART

(Please select quantity above )

Wishlist updated! View wishlist

Product Description

This is a highly cross-adsorbed rabbit anti-mouse IgG (H+L) secondary antibody labeled with our bright and photostable CF® dyes. To minimize cross-reactivity, the antibody has been adsorbed against human serum.

Highly cross-adsorbed for specific staining with minimal background
Available in 10 bright and photostable CF® dyes
Suitable for western, immunofluorescence, and immunohistology in FFPE tissues

Note: Conjugates of blue fluorescent dyes like CF®350 are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.