Do you offer alternatives to Akoya Opal™ tyramides?

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See below for our recommended alternatives to Akoya tyramides. Unless noted as a direct replacement, these tyramide dyes are spectrally similar alternatives. They have not been validated as direct replacements for use with Akoya kits or imaging systems.

CF® Dye Alternatives for Akoya Tyramides

Akoya Tyramide	Ex/Em (nm)	Biotium Tyramide	Ex/Em (nm)	Biotium Cat. No.	Notes	CF® Dye Features
TSA Coumarin	402/443	CF®405S	404/431	92197	Much brighter & more photostable alternative	CF®405S Features
Opal™ Polaris 480	450/500	CF®430	426/498	96053	Recommended for abundant targets	CF®430 Features
Opal™520	494/525	CF®488A	490/515	92171		CF®488A Features
TSA Fluorescein	494/517	Fluorescein	492/514	96018	Direct replacement	See CF®488A for a brighter & much more photostable alternative
TSA TMR TSA Cyanine 3	550/570	Cyanine 555	555/565	96020	Direct replacement for Cyanine 3	See CF®555 & CF®568 for brighter & more photostable alternatives.
		CF®555	555/565	96021	Brighter & more photostable alternatives	CF®555 Features
		CF®568	562/583	92173		CF®568 Features
Opal™570	550/570	CF®550R	551/577	96077		CF®550R Features
		CF®555	555/565	96021		CF®555 Features
TSA Plus Cyanine 3.5	581/596	CF®583R	586/609	96085	Brighter & more photostable alternatives	CF®583R Features
		CF®594	593/614	92174		CF®594 Features
Opal™620	588/616	CF®583R	586/609	96085		CF®583R Features
TSA Cyanine 5	648/667	CF®640R	642/662	92175	Brighter & more photostable alternative	CF®640R Features
		CF®647	650/665	96022	Brighter alternative	CF®647 Features
		CF®660R	663/682	92195	Less cross-talk with visible red dyes; bright & extremely photostable	CF®660R Features
TSA Plus Cyanine 5.5 Opal™690	673/692 676/694	CF®680R	680/701	92196	Bright & extremely photostable	CF®680R Features
Opal™ Polaris 780	750/770	CF®754	745/786	96090	Recommended for abundant targets	Unique NIR dye for TSA
TSA Biotin	N/A	Biotin-XX	N/A	92176	Direct replacement
TSA Plus DNP	N/A	DNP	N/A	96019	Direct replacement
TSA Plus DIG	N/A	N/A	N/A	N/A	N/A

Note: The CF® Dyes listed here are spectrally similar alternatives to Opal™ dyes, they have not been validated as direct replacements for use with Akoya's kits or imaging system. Opal is a trademark of Akoya Biosciences.

 

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