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α-Bungarotoxin, CF® Dye and Other Conjugates

Conjugates of α-Bungarotoxin labeled with a selection of our CF® Dyes and other labels. Labeled α-bungarotoxin conjugates can be used for staining nicotinic acetylcholine receptors at neuromuscular junctions in tissue sections.

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Product Attributes

For live or fixed cells

For fixed cells, For live/intact cells

Cell permeability

Membrane impermeant

Fixation options

Fix before staining (methanol), Fix before staining (formaldehyde)

Assay type/options

Tissue staining

Toxin

Alpha-bungarotoxin

Detection method/readout

Fluorescence microscopy

Colors

Blue, Green, Orange, Red, Far-red, Near-infrared

Storage Conditions

Store at -10 to -35 °C, Protect from light

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Size
Catalog #
price
Qty
100 ug
10 x 50 ug
500 ug
0.5 mg
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Product Description

Alpha-bungarotoxin is a polypeptide snake toxin that binds to the nicotinic acetylcholine receptor found at the neuromuscular junction with high affinity. Fluorescent alpha-bungarotoxin can be used for labeling of nicotinic acetylcholine receptors at neuromuscular junctions in tissue sections. Alpha-bungarotoxin may also be used for detection of GABA A receptor subsets in cells (1), or for labeling recombinant proteins that express the alpha-bungarotoxin binding site (BBS) epitope tag (2).

  • Choose from 9 bright and stable CF® Dye colors or other labels
  • Dye options for super-resolution and 2-photon imaging

We also offer Biotin-XX-alpha-bungarotoxin (catalog. no. 00017), and unconjugated alpha-bungarotoxin.(catalog no. 00010-1). See our complete selection of alpha-Bungarotoxins below.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.

Super-Resolution Microscopy

Many CF® Dyes are compatible with super resolution imaging, CF® Dyes give the best performance for multiple methods. The superior brightness, photostability, and photochemical switching properties of certain CF® Dyes are ideal for 3-D SIM, 3-D STORM, and other super-resolution and single-molecule imaging approaches. Learn more about CF® Dyes for super-resolution microscopy.

α-Bungarotoxin, CF® Dye and Other Conjugates

ConjugationEx/EmSizeCatalog No.Dye Features
UnconjugatedN/A1 mg00010-1
Biotin-XXN/A0.5 mg00017
CF®405S411/431 nm100 ug00002-100ugCF®405S Features
0.5 mg00002
CF®488A490/516 nm100 ug00005-100ugCF®488A Features
0.5 mg00005
CF®543 543/563 nm100 ug00026-100ugCF®543 Features
0.5 mg00026
CF®555554/568 nm100 ug00018-100ugCF®555 Features
0.5 mg00018
CF®568562/584 nm100 ug00006-100ugCF®568 Features
0.5 mg00006
CF®594593/615 nm100 ug00007-100ugCF®594 Features
0.5 mg00007
CF®633629/650 nm100 ug00009-100ugCF®633 Features
0.5 mg00009
CF®640R642/663 nm100 ug00004-100ugCF®640R Features
0.5 mg00004
CF®680R680/701 nm100 ug00003-100ugCF®680R Features
0.5 mg00003
Fluorescein
(FITC)
498/517 nm0.5 mg00011
10 x 50 ug00013
Tetramethylrhodamine
(TRITC)
552/578 nm0.5 mg00012
10 x 50 ug00014
Sulforhodamine-101
(Texas Red®)
595/613 nm0.5 mg00015
10 x 50 ug00016

 

CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific.

References

  1. PNAS, 103, 13, (2006), DOI: 10.1073/pnas.0600847103
  2. Meth. Enzymol., 521, (2013), DOI: 10.1016/B978-0-12-391862-8.00006-5
  3. Sci Adv, 6, 15, (2020), DOI: 10.1126/sciadv.aax8382
  4. Am J Physiol Cell Physiol, (2020) DOI: 10.1152/ajpcell.00453.2019
  5. Biochem Biophys Res Commun., 523, 214, (2020), DOI: 10.1016/j.bbrc.2019.12.011
  6. Front Cell Dev Biol., 8, 15, (2020), DOI: 10.3389/fcell.2020.00015
  7. Biophysics, 64, 772, (2019), DOI: 10.1134/S0006350919050129
  8. Cell Physiol Biochem, 53, 701, (2019), DOI: 10.33594/000000166
  9. Biochemistry (Mosc.), 4, 1085, (2019), DOI: 10.1134/S0006297919090116
  10. Biomaterials, 225, 119537, (2019), DOI: 10.1016/j.biomaterials.2019.119537
  11. ACS Chem. Biol., 13, 2568, (2018), DOI: 10.1021/acschembio.8b00513
  12. Cell Mol Neurobiol, 37, 1443, (2017), DOI: 10.1007/s10571-017-0475-3
  13. Neuroscience, 174, 234, (2011), DOI: 10.1016/j.neuroscience.2010.11.016
  14. Am J of Pathol, 177, 2509 (2010), DOI: 10.2353/ajpath.2010.100243
  15. Cell Tissue Biol, 4, 258, (2010), DOI: 10.1134/S1990519X10030077
  16. Neuroscience, 174, 234, (2009), DOI: 10.1016/j.neuroscience.2010.11.016
  17. J Cell Biol, 150, 1385, (2000), DOI: 10.1083/jcb.150.6.1385
  18. Neuron, 23, 675, (1999), DOI: 10.1016/s0896-6273(01)80027-1
  19. Neuron, 12, 167, (1994), DOI: 10.1016/0896-6273(94)90161-9
  20. J Cell Biol, 125, 661, (1994), DOI: 10.1083/jcb.125.3.661
  21. J Biol Chem, 268, 25108, (1993), PMID: 8227074
  22. Muscle Nerve, 5, 140, (1982), DOI: 10.1002/mus.880050211
  23. PNAS 77, 4823, (1980), DOI: 10.1073/pnas.77.8.4823
  24. Science, 196, 540, (1977), DOI: 10.1126/science.850796

Download a list of CF® dye references.

 

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