Buffers, Accessories, & Background Reducers

Concentrated buffer used for Annexin V staining of cells to monitor apoptosis.

Additional concentrated cell lysis buffer that is designed for use with Biotium’s Firefly Luciferase Assay Kits when performing large sample volume assays.

Additional concentrated lysis buffer for use with our Renilla Luciferase Assay Kits in large volume assays.

Ac-DEVD-CHO is a synthetic tetrapeptide competitive inhibitor for Caspase-3/7, and contains the amino acid sequence of the PARP cleavage site. Ac-DEVD-CHO can be used to inhibit Caspase-3/7 activity in apoptotic cells, and to study events downstream of Caspase-3/7 activation.

This kit provides buffers for generating a range of accurate calcium concentrations for the calibration of fluorescent Ca²⁺ indicators.

Zwitterionic non-denaturing detergent for solubilizing membrane proteins for protein gels and assays.

Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.

EDC (or EDAC) (1-(3-Dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)) has recently been found to be very useful for fixing in situ chelators, including the fluorescent ion indicators for histological studies following physiological experiments. EDC is also a widely used reagent to activate carboxy groups for amine coupling.

Nonionic detergent useful for solubilizing hydrophobic compounds to facilitate cell loading.

Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic dye molecules such as AM esters of calcium indicator dyes to facilitate cell loading.

Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic compounds like AM esters of calcium indicator dyes to facilitate cell loading.

Probenecid is commonly used to inhibit organic-anion transporters located in the cell membrane. Such transporters can extrude dyes and indicators and thus contribute to low signal or high background in assays due to poor cellular retention of dyes.

SCAS (4-Sulfonato calix[8]arene, sodium salt) is a quencher developed by Biotium to reduce background fluorescence when using our fixable AM dyes, SynaptoGreen and SynaptoRed dyes. Unlike ADVASEP-7, SCAS dramatically lowers background fluorescence as soon as it is added to the preparation without the need for repeated wash steps.

Staurosporine is a potent and cell-permeable inhibitor of a wide variety of protein kinases by competing for the ATP binding site.

TCEP hydrochloride is an odorless reducing agent that is more stable and effective than dithiothreitol (DTT) or 2-Mercaptoethanol. TCEP hydrochloride is soluble in water to 310 grams per liter.