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Buffers, Accessories, & Background Reducers

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    Ready-to-Use Tyramide Amplification Buffer, 1X

    Sizes: 25 mL, 100 mLCatalog #: 22027-25mL, - 22027-100mLView allHide

    Ready-to-use buffer for tyramide signal amplification; simply add CF® dye or other tyramide probes to the buffer for signal amplification.

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    25 mL
    100 mL
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    Ready-to-use buffer for tyramide signal amplification; simply add CF® dye or other tyramide probes to the buffer for signal amplification.

     

    5X Annexin V Binding Buffer

    From: $28 Sizes: 15 mLCatalog #: 99902

    Concentrated Annexin V Binding Buffer to be used for Annexin V staining.

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    15 mL
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    Concentrated Annexin V Binding Buffer to be used for Annexin V staining.

     

    5X Firefly Luciferase Lysis Buffer

    From: $19 Sizes: 15 mLCatalog #: 99923

    5X Firefly Lysis Buffer is a concentrated cell lysis buffer that is designed for use with Biotium’s Firefly Luciferase Assay Kit  and Firefly Luciferase Assay Kit (Lyophilized).

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    15 mL
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    5X Firefly Lysis Buffer is a concentrated cell lysis buffer that is designed for use with Biotium’s Firefly Luciferase Assay Kit  and Firefly Luciferase Assay Kit (Lyophilized).

     

    5X Passive Lysis Buffer

    From: $25 Sizes: 30 mLCatalog #: 99912

    5X Passive Lysis Buffer is concentrated lysis buffer designed for use with Biotium’s Renilla Luciferase Assay Kit (30004) or Firefly and Renilla Dual Luciferase Assay Kit (30005).

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    30 mL
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    5X Passive Lysis Buffer is concentrated lysis buffer designed for use with Biotium’s Renilla Luciferase Assay Kit (30004) or Firefly and Renilla Dual Luciferase Assay Kit (30005).

     

    Ac-DEVD-CHO Caspase-3 Inhibitor

    From: $70 Sizes: 1 mg, 5 mgCatalog #: 10404, - 10404-1View allHide

    Ac-DEVD-CHO is a synthetic tetrapeptide competitive inhibitor for Caspase-3/7, and contains the amino acid sequence of the PARP cleavage site. Ac-DEVD-CHO can be used to inhibit Caspase-3/7 activity in apoptotic cells, and to study events downstream of Caspase-3/7 activation.

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    1 mg
    5 mg
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    Ac-DEVD-CHO is a synthetic tetrapeptide competitive inhibitor for Caspase-3/7, and contains the amino acid sequence of the PARP cleavage site. Ac-DEVD-CHO can be used to inhibit Caspase-3/7 activity in apoptotic cells, and to study events downstream of Caspase-3/7 activation.

     

    Calcium Calibration Buffer Kit

    From: $156 Sizes: 1 kitCatalog #: 59100

    This kit provides buffers for generating a range of accurate calcium concentrations for the calibration of fluorescent Ca2+ indicators.

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    1 kit
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    This kit provides buffers for generating a range of accurate calcium concentrations for the calibration of fluorescent Ca2+ indicators.

     

    CHAPS, Ultra Pure

    Sizes: 10 g, 100 gCatalog #: 22024-10g, - 22024-100gView allHide

    Zwitterionic non-denaturing detergent for solubilizing membrane proteins.

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    10 g
    100 g
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    Zwitterionic non-denaturing detergent for solubilizing membrane proteins.

     

    DMSO, Anhydrous

    From: $22 Sizes: 10 mLCatalog #: 90082

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

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    10 mL
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    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

     

    DTT

    From: $33 Sizes: 1 gCatalog #: 91050

    DTT

    DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.

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    1 g
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    DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.

     

    EDC (EDAC)

    From: $71 Sizes: 100 mgCatalog #: 59002

    EDC (or EDAC) (1-(3-Dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)) has recently been found to be very useful for fixing in situ chelators, including the fluorescent ion indicators for histological studies following physiological experiments. EDC is also a widely used reagent to activate carboxy groups for amine coupling.

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    100 mg
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    EDC (or EDAC) (1-(3-Dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)) has recently been found to be very useful for fixing in situ chelators, including the fluorescent ion indicators for histological studies following physiological experiments. EDC is also a widely used reagent to activate carboxy groups for amine coupling.

     

    Pluronic® F-127

    From: $19 Sizes: 1 gCatalog #: 59000

    Nonionic detergent useful for solubilizing hydrophobic compounds to facilitate cell loading.

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    1 g
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    Nonionic detergent useful for solubilizing hydrophobic compounds to facilitate cell loading.

     

    Pluronic® F-127 20% Solution in DMSO

    From: $15 Sizes: 1 mLCatalog #: 59004

    Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic dye molecules such as AM esters of calcium indicator dyes to facilitate cell loading.

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    1 mL
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    Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic dye molecules such as AM esters of calcium indicator dyes to facilitate cell loading.

     

    Pluronic® F-127, 10% in H2O

    From: $48 Sizes: 30 mLCatalog #: 59005

    Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic compounds like AM esters of calcium indicator dyes to facilitate cell loading.

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    30 mL
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    Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic compounds like AM esters of calcium indicator dyes to facilitate cell loading.

     

    Probenecid, Sodium Salt, Water Soluble

    From: $173 Sizes: 10 x 77 mgCatalog #: 50027

    Probenecid is commonly used to inhibit organic-anion transporters located in the cell membrane. Such transporters can extrude dyes and indicators and thus contribute to low signal or high background in assays due to poor cellular retention of dyes.

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    10 x 77 mg
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    Probenecid is commonly used to inhibit organic-anion transporters located in the cell membrane. Such transporters can extrude dyes and indicators and thus contribute to low signal or high background in assays due to poor cellular retention of dyes.

     

    Staurosporine

    From: $83 Sizes: 100 ugCatalog #: 00025

    Staurosporine is a potent and cell-permeable inhibitor of a wide variety of protein kinases (1,2) including protein kinase C (IC50 = 0.7 nM), protein kinase A (IC50 = 7 nM), and protein kinase G (IC50 = 8.5 nM). Staurosporine induces apoptosis in human neuroblastoma cells and chick embryonic neurons (3,4).

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    100 ug
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    Staurosporine is a potent and cell-permeable inhibitor of a wide variety of protein kinases (1,2) including protein kinase C (IC50 = 0.7 nM), protein kinase A (IC50 = 7 nM), and protein kinase G (IC50 = 8.5 nM). Staurosporine induces apoptosis in human neuroblastoma cells and chick embryonic neurons (3,4).

     

    TCEP

    From: $71 Sizes: 1 gCatalog #: 91049

    TCEP hydrochloride is an odorless reducing agent that is more stable and effective than dithiothreitol (DTT) or 2-Mercaptoethanol. TCEP hydrochloride is soluble in water to 310 grams per liter.

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    1 g
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    TCEP hydrochloride is an odorless reducing agent that is more stable and effective than dithiothreitol (DTT) or 2-Mercaptoethanol. TCEP hydrochloride is soluble in water to 310 grams per liter.