Menu
close
biotium logo
Antibody Finder
((0 ITEMS) ITEMS)
$0
Menu
close
Hero Image

Antibody & Protein Labeling Kits

Efficient, easy-to-use kits for labeling antibodies and other proteins with our superior CF® Dyes and other labels.

Fast & Easy Antibody Labeling

Easy-to-use, with minimal hands-on time, Mix-n-Stain™ kits require no purification after labeling and conjugates are stable for storage.

Kits for labeling IgM

Mix-n-Stain™ kits with optimized reaction conditions for efficient and bright labeling of IgM antibodies with CF® Dyes.

Optimal Labeling of Nanobodies®

Mix-n-Stain™ kits specific for labeling Nanobodies® (single variable or VHH domains)

Optimal Labeling for STORM

Your choice of 8 STORM CF® Dyes for labeling antibodies with a low DOL optimal for STORM super-resolution microscopy

Small Ligand Labeling Kits

Create fluorescent SNAP-tag®, CLIP-tag™, Halotag® and other ligands, no purification required

Protein Labeling Kits

Larger scale kits, for optimized antibody and protein labeling with CF® Dyes and subsequent purification

VivoBrite™ Near-IR Labeling Kits

Kits for labeling antibodies with our superior near-IR CF® Dyes, for in vivo small animal imaging

Mix-n-Stain™ CF® Dye Antibody Labeling Technology

Mix-n-Stain™ Antibody Labeling Kits

Even though antibody conjugation is dramatically simplified, the conjugates perform comparably to pre-labeled antibodies from leading suppliers. Covalent labeling ensures conjugates are stable for long-term storage, and are ideal for multi-color imaging and multiplex flow cytometry. Each kit includes everything you need, and works with most commercial antibody formulations.

Rapid Antibody Labeling with CF® Dyes & More

Mix-n-Stain™ CF® Dye Antibody Labeling Kits covalently label antibodies with 100% recovery. CF® Dyes offer superior brightness and photostability versus other fluorescent dyes. High water solubility of the dyes minimizes non-specific background as non-IgG proteins wash away during IF staining. 

Mix-n-Stain™ kits allow antibody labeling directly in common buffers containing BSA, gelatin, azide, or low Tris, and with minor protocol adjustments, even in BSA, gelatin, or ascites fluid. This eliminates the need for antibody purification, making the kits compatible with most commercial formulations. See the the Product Protocol for step-by-step compatibility guidance.

Features

  • 3 kit sizes: 5–20 µg, 20–50 µg, or 50–100 µg Ab
  • Maxi Antibody Labeling Kits for 1 mg scale labeling reactions
  • Choice of over 30 CF® Dye colors, biotin, FITC, or haptens
  • Less than 30 seconds of hands-on time
  • 15 minutes total reaction time
  • No purification of the product is required
  • Compatible with common antibody stabilizers

Compatible with antibody stabilizers and ascites

Compatible with common antibody stabilizers and ascites. Monoclonal mouse anti-tubulin clone DM1A in ascites or purified in PBS with 1% BSA was labeled with CF®... See More
combined direct and indirect immunofluorescence staining of rat testis for microtubules, actin filaments and tight junctions
Combine direct and indirect immunofluorescence for flexible multiplexing. rat testis were stained with mouse anti-tubulin and CF®488A goat anti-mouse (min x rat... See More
Mouse anti-transferrin receptor antibody from (endosome and plasma membrane marker) was labeled using Lightning-Link® Rapid DyLight® Conjugation Kits from Novus... See More

Mix-n-Stain™ CF® Dye, Biotin, FITC & Hapten IgG Antibody Labeling Kits

Product descriptionLabel optionsLabeling time
Mix-n-Stain™ CF® Dye Antibody Labeling KitsChoice of over 30 bright & photostable CF® Dyes15 minutes (~30 seconds hands-on time)

Download the product protocol
Mix-n-Stain™ Cyanine Dye
Antibody Labeling Kits		Cyanine 555
Cyanine 647
Mix-n-Stain™ FITC Antibody Labeling KitsFITC
Mix-n-Stain™ Biotin Antibody Labeling KitsBiotin
Mix-n-Stain™ DNP Antibody Labeling KitsDNP
Mix-n-Stain™ Digoxigenin Antibody Labeling KitsDigoxigenin (DIG)
Mix-n-Stain™ Maxi Antibody Labeling KitsChoice of 13 bright & photostable CF® Dyes, FITC, Cyanine 555, and Cyanine 64730 minutes (~30 seconds hands-on time)
Mix-n-Stain™ STORM CF® Antibody Labeling KitsChoice of 8 CF® Dyes specialized for STORM super-resolution microscopy

Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits

Finally! Streamlined Labeling of IgM Antibodies

IgM antibodies are difficult to label with fluorescent dyes because the conditions typically used for labeling IgG generally do not result in bright conjugates with good antibody reactivity for IgM. Biotium has resolved this issue by optimizing the reaction conditions for labeling IgM antibodies with Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits.

Features

  • Label 25 µg or 100 µg of IgM antibody
  • Choice of 8 CF® Dye colors and FITC
  • 15–30 minute total reaction time
  • No purification, 100% recovery
Mouse anti-beta tubulin clone 5H1 (IgM kappa) was labeled with CF®488A using Mix-n-Stain™ IgM labeling, then was used to stain methanol-fixed HeLa cells at 5 ug... See More
Mouse monoclonal anti-CD19 antibody (clone PDR134, IgM kappa isotype) was labeled with CF®488A using Mix-n-Stain™ IgM labeling. The conjugate was used to stain ... See More

Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits

ConjugationEx/EmSizeCatalog No.Dye Features
CF®405L413/547 nm25 ug92558CF®405L Features
100 ug92559
CF®405M416/452 nm25 ug92560CF®405M Features
100 ug92561
CF®488A490/516 nm25 ug92562CF®488A Features
100 ug92563
CF®555554/568 nm25 ug92564CF®555 Features
100 ug92565
CF®568562/583 nm25 ug92566CF®568 Features
100 ug92567
CF®594593/615 nm25 ug92568CF®594 Features
100 ug92569
CF®640R642/663 nm25 ug92570CF®640R Features
100 ug92571
CF®647652/668 nm25 ug92572CF®647 Features
100 ug92573
FITC498/517 nm25 ug92574
100 ug92575
Note: Before selecting a kit, please download the Product Protocol to see Kit Compatibility and Protocol Selection guidelines.

Mix-n-Stain™ Nanobody Labeling Kits

Nanobodies® (also called camelid single variable or VHH domains), are smaller ~15 kDa single-domain antibodies that enable deeper tissue penetration, faster staining times, and improved access to tightly packed or masked epitopes—making them ideal for high-resolution and super-resolution imaging.

Biotium offers Nanobody® labeling kits designed specifically for labeling single-domain Nanobodies® with one of Biotium’s bright and photostable CF® Dyes.

Mix-n-Stain™ Nanobody Labeling Kits are for standard labeling of VHH, while the Mix-n-Stain™ Nanobody Thiol Labeling Kits were designed for labeling VHH containing a single cysteine (1x Cys) residue. The kits allow optimal labeling of between 5-50 ug of Nanobody®, with minimal hands-on time and no purification.

 

Biotium also offers MiniMab™ single-domain antibodies (SdAbs) labeled with best-in-class CF® Dyes for STORM. MiniMab™ SdAbs are available for popular neuroscience targets or as secondary antibodies.

Mix-n-Stain™ Nanobody Labeling Kit Features

  • Ideal for standard labeling of VHH
  • Label 5-20 ug or 20-50 ug Nanobody®
  • Choice of 7 CF® Dye colors or biotin
  • Simple 30 minute labeling, no purification
  • Compatible with common stabilizers
  • Prevents over-labeling

 

Mix-n-Stain™ Nanobody Thiol Labeling Kits Features

  • Designed for labeling VHH with a single cysteine (1x Cys) residue
  • Label 5-20 ug or 20-50 ug Nanobody®
  • Choice of 7 CF® Dye colors
  • Simple 2 hour labeling, no purification
  • No purification, 100% recovery
Anti-histone Nanobody® was labeled using the Mix-n-Stain™ CF®488A 5-20 ug Nanobody Labeling Kit, then used to stain methanol-fixed HeLa cells at 2 ug/mL. Labeli... See More

Mix-n-Stain™ Nanobody Labeling Kits

LabelEx/EmLabeling size
(ug Nanobody®)		Catalog number
BiotinN/A5-20 ug92500
20-50 ug92501
CF®405S404/431 nm5-20 ug92502
20-50 ug92503
CF®488A490/515 nm5-20 ug92504
20-50 ug92505
CF®568562/583 nm5-20 ug92506
20-50 ug92507
CF®594593/614 nm5-20 ug92508
20-50 ug92509
CF®640R642/662 nm5-20 ug92510
20-50 ug92511
CF®647650/665 nm5-20 ug92512
20-50 ug92513
CF®680R680/701 nm5-20 ug92514
20-50 ug92515
Note: Before selecting a kit, please download the Product Protocol to see Kit Compatibility and Protocol Selection guidelines.

Mix-n-Stain™ Nanobody Thiol Labeling Kits

ConjugationEx/EmLabeling size
(ug Nanobody®)		Catalog No.Dye Features
CF®488A490/516 nm5-20 ug92585CF®488A Features
20-50 ug92586
CF®568562/584 nm5-20 ug92587CF®568 Features
20-50 ug92588
CF®583R585/609 nm5-20 ug92589CF®583R Features
20-50 ug92590
CF®594593/615 nm5-20 ug92591CF®594 Features
20-50 ug92592
CF®647652/668 nm5-20 ug92593CF®647 Features
20-50 ug92594
CF®680681/698 nm5-20 ug92595CF®680 Features
20-50 ug92596
CF®740742/767 nm5-20 ug92597CF®740 Features
20-50 ug92598
Note: Before selecting a kit, please download the Product Protocol to see Kit Compatibility guidelines.

Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits

Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits offer a choice of 8 CF® Dyes developed with superior brightness and unique photoswitching properties that are ideal for super-resolution imaging by STORM. In addition, these specialized antibody labeling kits are designed to offer a low degree of labeling (DOL), with 1-2.5 dye molecules per antibody molecule, which is reported to be optimal for STORM (Bittel et al. 2015). Biotium has also developed novel green-excited STORM dyes CF®583R and CF®597R to expand the color palette for multi-color STORM. Learn more about the UC Berkeley and Biotium collaboration paper on CF®583R and CF®597R dyes for STORM, and other fluorescent reagents validated for super-resolution microscopy.

Typical (d)STORM image of the actin cytoskeleton in a fixed COS-7 cell labeled by phalloidin-CF®583R. (d)STORM was performed under standard conditions using a s... See More
Features
  • Choice of 8 CF® Dye colors ideal for STORM
  • CF®583R and CF®597R are novel green-excited STORM dyes
  • Labeling optimized to provide low 1–2.5 DOL
  • Less than 30 seconds of hands-on time
  • 30 minutes total reaction time
  • No purification, 100% recovery

Two Color (d)STORM Imaging of a Fixed COS-7 Cell

Two-color (d)STORM imaging of a fixed COS-7 cell: microtubules were immunolabeled with CF®647 conjugate, and mitochondria were immunolabeled with CF®583R conjug... See More

Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits

ConjugationEx/EmSizeCatalog No.Dye Features
CF®505505/519 nm50 ug92549
CF®568562/584 nm50 ug92550CF®568 Features
CF®583R585/609 nm50 ug92551CF®583R Features
CF®597R597/619 nm50 ug92553CF®597R Features
CF®647652/668 nm50 ug92554CF®647 Features
CF®660C667/685 nm50 ug92555CF®660C Features
CF®680681/698 nm50 ug92556CF®680 Features
CF®750755/779 nm50 ug92557CF®750 Features

Mix-n-Stain™ CF® Dye Small Ligand Labeling Kits

Mix-n-Stain™ CF® Dye small ligand labeling kits are designed for rapid labeling of small (MW ~ 150 – 5,000) and relatively high affinity biological ligands (or substrates). Labeling takes about 30 minutes, without a final purification step. The ligands to be labeled must contain an aliphatic amine group that is not required for biological activity of the ligand. The amine group will form a covalent linkage with the reactive CF® Dye provided in the kit. For example, suitable ligands or substrates include SNAP-tag®, CLIP-tag™ and HaloTag® ligands with an aliphatic amine. Many other small ligands are also possible candidates if they meet the criteria described above.

For live cell imaging, the Mix-n-Stain™ small ligand kits are ideal to generate fluorescent chemical tags that provide smaller, brighter, and more versatile alternatives to fluorescent proteins. Labeling various chemical tags with Mix-n-Stain™ kits yielded highly efficient and specific labeling of the target proteins both on the surface and inside of living cells. The Mix-n-Stain™ small ligand kits have also been proven compatible with fixed cell imaging and gel-based assays.

Figure 1. Live cell imaging of HeLa cells expressing CLIP-NK1R labeled with (A) CLIP-tag™ ligand conjugated to green fluorescent CF®488A using Mix-n-Stain™ comp... See More
Features
  • Choice of 10 CF® Dye colors for extracellular staining
  • Choice of 5 CF® Dye colors for intracellular staining
  • For labeling molecules with a MW of 150–5000, containing an inactive aliphatic amine
  • Suitable ligands or substrates include SNAP-tag®, CLIP-tag™ and HaloTag® ligands
  • 30 minutes total reaction time, no purification necessary

Cell Surface and Intracellular Labeling Options

Figure 2. Three color imaging in live HeLa cells. Cell surface protein ADRβ2 was labeled using the CF®488A Mix-n-Stain™ kit via the SNAP-tag® (green). Mitochond... See More
Figure 3. CLIP-tag™ ligand was labeled with the CF®488A Mix-n-Stain™ Small Ligand Labeling Kit, then used to detect cell surface expression of CLIP-NK1R protei... See More
Simply mix your small molecule ligand with the reaction buffer and the optimally formulated dye provided, and incubate for 30 minutes, followed by a brief 5 minute quenching step. No reactive Mix-n-Stain™ dye is available at the end of labeling; therefore the conjugate is ready for cell staining and imaging without further purification. Mix-n-Stain™ labeled ligands perform comparably to commercially-available pre-labeled ligands, at a fraction of the cost.

Mix-n-Stain™ Small Ligand Kits

Product descriptionLabel optionsLabeling time
Mix-n-Stain™ CF® Dye Small Molecule Labeling KitsExtracellular Staining: Choice of 10 bright and stable CF® Dyes~30 minutes (minimal hands-on time)

Download the product protocol
Intracellular Staining: Choice of 5 bright and stable CF® Dyes

CF® Dye SE Protein Labeling Kits

CF® Dye SE Protein Labeling Kits are designed for 3 labelings of up to 1 mg of protein or antibody with bright, photostable CF® Dyes. Unlike Mix-n-Stain™ kits, which are optimized for consistent labeling of small amounts of antibody, the SE Protein Labeling kits allow dye removal after labeling and optimization of dye-to-protein ratio. Ideal for users needing control over labeling conditions and antibody performance.

Features

  • Choice of 19 CF® Dye colors or biotin
  • Reagents for 3 labeling reactions with purification
  • Each reaction can label up to 1 mg of antibody or protein
  • Includes instructions for determining degree of labeling

VivoBrite™ Near-IR Antibody Labeling Kits

Label Antibodies for In Vivo Imaging

VivoBrite™ Antibody Labeling Kits use the same labeling technology as our SE Protein Labeling Kits. However, the kits include a syringe and syringe filter to sterile filter the labeled protein, allowing it to be used for in vivo small animal studies. Biotium’s near-IR CF® Dyes are best in class for brightness, photostability, and water solubility.

Features

  • Choice of 4 near-IR CF® Dye colors optimal for small animal in vivo imaging
  • Kit contains reagents for 3 labeling reactions
  • Each reaction can label up to 1 mg of antibody
  • Kit can also be used to label other proteins
  • The resulting labeled protein is purified and sterile

Protein Labeling Kits

Product descriptionLabel optionsProduct protocol
CF® Dye SE Protein Labeling Kits19 CF® Dye colors across the spectrum, plus biotinPI-CF® Dye SE Protein Labeling Kits
VivoBrite™ Antibody Labeling Kits for Small Animal In Vivo Imaging4 near-IR CF® DyesPI-VivoBrite™ Antibody Labeling Kits

SNAP-tag is a registered trademark of New England Biolabs; CLIP-tag is a trademark of New England Biolabs; HaloTag is a registered trademark of Promega; Nanobody is a registered trademark of Ablynx.

Product Spotlight

Resources That Fuel Bright Ideas

View All Resources

Related Solutions

Mix-n-Stain™ Antibody Labeling Kits

Simplify and accelerate covalent antibody labeling with flexible dye, enzyme, and protein tag options—always stable, reliable, and high-performing.

CF® Dye Protein Labeling Kits

Achieve stable succinimidyl ester (SE) antibody or protein conjugation with your choice of biotin or 19+ CF® Dyes spanning the UV to near-IR spectrum.

Reactive Fluorescent Dyes

CF® Dyes, reactive dyes, and efficient biotinylation reagents. With fluorescent dyes delivering exceptional brightness across the visible to near-IR spectra to create high-performance tools for flow cytometry, ELISA, and beyond.

In Vivo Imaging

Drive discovery with bright, photostable in vivo imaging reagents that deliver unrivaled signal-to-noise. Biotium’s in vivo imaging products include NIR CF® Dyes which offer important advantages over competitor NIR dyes and traditional visible dyes.

Cell & Organelle Stains

Achieve bright, clean staining with innovative dyes optimized for live, dead, and fixed cells. With colors spanning the visible to near-IR spectrum, our stains offer low-background, high-contrast imaging for diverse targets and applications.

FAQs

See our CF® Dye Quick Reference Table for a list of dyes and summary of their features. Our CF® Dye Selection Guide has more detailed information on each CF® Dye, and ordering information for our various CF® Dye product lines. You can download the normalized absorption and emission spectra of all CF® Dyes in csv format from the spectra viewer.

For CF® dye, cyanine dye, FITC, biotin, DNP, and dig kits, see the Mix-n-Stain™ Kit Compatibility and Protocol Selection Flowchart, below. We also recommend downloading the updated Product Protocol and completing the pre-labeling checklist to find the right protocol and kit size to use for each antibody you wish to label. Learn more about CF® dyes on our CF® Dye Technology Page, or download the CF® Dye Brochure.

Note that Mix-n-Stain™ Maxi 1 mg Scale Kits, Mix-n-Stain™ Fluorescent Protein Labeling Kits (R-PE, APC, PerCP, or tandem dye), Mix-n-Stain™ Enzyme Labeling Kits (AP, HRP, GOx) have different compatibility requirements and labeling protocols. Check the protocol for your kit to verify that your antibody is compatible with labeling, then choose a kit size that matches the amount of antibody you wish to label.

Mix-n-Stain™ labeling results in covalent linkage of dye and antibody, so there will be no dye diffusion or transfer.

View more FAQs
Can't find your answer?
Talk to an Expert
New Products

Antibodies, Conjugates, & Labeling
Cellular Stains & Assays
Molecular Biology
Protein Detection & Analysis
Enzyme Substrates & Assays
Reagents & Accessories by Research Area
Reagents & Accessories by Application

Content #1

Content #1

Content #1

Content #2

Content #3