5-(and-6)-Carboxyfluorescein
5-(and-6)-FAM (full name: 5-(and-6)-Carboxyfluorescein mixed isomer) has a pKa of 6.5 and can be used as a pH indicator.
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Product Description
5-(and-6)-FAM (full name: 5-(and-6)-Carboxyfluorescein mixed isomer) has a pKa of 6.5 and can be used as a pH indicator. Its excitation spectrum and fluorescence response to pH are similar to those of BCECF. Carboxyfluorescein can also be used as a tracer dye. The dye is membrane-impermeant and can be loaded into cells by microinjection or scrape loading.
- λEx/λEm (pH 9) = 492/514 nm
- ε (pH 9) = 82,000
- Yellow orange solid soluble in pH>6 water
- Store at 4°C and protect from light, especially in solution
- C21H12O7
- MW: 376
- [72088-94-9]
References
1. Meth Enzymol 128, 65 (1986).
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5-(and 6)-CFDA
5(6)-CFDA (full name: 5-(and-6)-Carboxyfluorescein diacetate) is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, CFDA is hydrolyzed by intracellular esterases to carboxyfluorescein (see #51013 ).
5-(and-6)-Carboxy-2′,7′- dichlorofluorescein
5-(and-6)-carboxy-2',7'-dichlorofluorescein is similar to carboxyfluorescein (51013), but has lower pKa. It is useful for detecting pH in more acidic environment.
CDCFDA
5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate is membrane-permeant and thus can be loaded into cells via incubation. The diacetate is readily hydrolyzed to 5-(and-6)-carboxy-2',7'-dichlorofluorescein (51015) by intracellular esterases.
5-Carboxy-2′,7′-dichlorofluorescein (single isomer)
This dye can be used as a low pH indicator similar to 51015, and is also useful as a general green fluorescent label.
5-CFDA (5-Carboxyfluorescein diacetate, single isomer)
5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein (see 50015).