CF® Dye BCN
CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labeling reactions are also available.
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Product Description
CF® Dye BCN (bicyclo[6.1.0]nonyne) derivatives are alternatives to fluorescently labeled DIBO and DBCO. They react with azides to form 1,2,3-triazole by copper-free bioorthogonal 1,3-dipolar Huisgen cycloaddition. BCN dyes are useful for fluorescent labeling of live cell surface or cell extracts, particularly when there are concerns about native protein function loss with copper. Membrane-permeant CF® Dye BCN options are also available for intracellular labeling reactions.
- Fluorescent labeling of azide groups on target molecules via copper-free bioorthogonal chemistry.
- Alternative to fluorescently labeled DIBO and DBCO.
- Suitable for surface or intracellular labeling of live cells or cell extracts.
- Bright, photostable and water-soluble CF® Dyes are excellent options for fluorescent labeling.
- Available in several CF® Dye options including membrane-permeant forms for intracellular labeling.
Biotium also offers reactive CF® Dye DBCO derivatives which react more rapidly with azide groups than other cyclooctynes through strain-promoted azide-alkyne cycloaddition (SPAAC).
Superior CF® Dyes
Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes. For more information download the CF® Dye Brochure.
CF® Dye BCN
CF® Dye BCN | Ex/Em | Membrane Permeability | Size | Catalog No. | Dye Features |
---|---|---|---|---|---|
CF®405S | 404/431 nm | Impermeant | 0.5 mg | 92113 | CF®405S Features |
CF®405M | 408/452 nm | Impermeant | 0.5 mg | 92114 | CF®405M Features |
CF®440 | 440/515 nm | Permeant | 0.5 mg | 96070 | CF®440 Features |
CF®488A | 490/515 nm | Impermeant | 0.5 mg | 92075 | CF®488A Features |
CF®500 | 502/511 nm | Permeant | 0.5 mg | 96026 | |
CF®568 | 562/583 nm | Impermeant | 0.5 mg | 92076 | CF®568 Features |
CF®594 | 593/614 nm | Impermeant | 0.5 mg | 92077 | CF®594 Features |
CF®640R | 642/662 nm | Impermeant | 0.5 mg | 92078 | CF®640R Features |
CF®647 | 650/665 nm | Impermeant | 0.5 mg | 96059 | CF®647 Features |
CF®650 | 653/673 nm | Permeant | 0.5 mg | 96027 | |
CF®680 | 681/698 nm | Impermeant | 0.5 mg | 96058 | CF®680 Features |
CF®680R | 680/701 nm | Impermeant | 0.5 mg | 92079 | CF®680R Features |
References
1. Canadian Journal of Chemistry (2019),97(1):1-6. DOI: 10.1139/cjc-2018-0253.
2. eLife (2019),8: e50776. DOI: 10.7554/eLife.50776.001
3. Chem. Mater. (2019), 31:8035−8043. DOI: 10.1021/acs.chemmater.9b02485
4. Advanced functional materials, (2016),26(21):3612-3620. DOI: 10.1002/adfm.201505329
Download a list of CF® dye references.