Menu
close
biotium logo
Antibody Finder
((0 ITEMS) ITEMS)
$0
Menu
close

MitoView™ Mitochondrial Dyes

Fluorogenic mitochondrial stains for live cells that rapidly accumulate in mitochondria and can be imaged without washing.

Dye
Size
Catalog #
price
Qty
Trial size (50 ug)
20 x 50 ug
Clear selection
Add to Cart
This product is available by special order only.

Please fill in the inquiry form and we will contact you shortly.
Don't see what you're looking for? Contact us for more conjugation options.
Request Quote or Bulk Order
MitoView™ Mitochondrial Dyes
MitoView™ Mitochondrial Dyes - Image 2Live HeLa cells stained with MitoView™ Green (mitochondria, green) and LysoView™ 633 (lysosomes, magenta).
MitoView™ Mitochondrial Dyes - Image 3MitoView™ 405
MitoView™ DyesMitoView™ Green
MitoView™ Mitochondrial Dyes - Image 5MitoView™ 633
MitoView™ Mitochondrial Dyes - Image 6MitoView™ 720 in live HeLa cells, imaged in the Cy®5 channel.
MitoView™ Mitochondrial Dyes - Image 7Measurement of mitochondrial membrane potential by Jurkat cells using MitoView™ 633 and flow cytometry. Jurkat cells were left untreated, or treated with 50 uM CCCP for 10 minutes to depolarize mitochondrial membrane potential. Cells were stained with 150 nM MitoView™ 633 for 30 minutes and analyzed in the FL4 channel of a BD FACSCalibur flow cytometer. Cells treated with CCCP (blue) show a decrease in staining intensity with MitoView™ 633 compared to DMSO-treated control cells (red).
MitoView™ Mitochondrial Dyes - Image 8Flow cytometry analysis of Jurkat cells treated with staurosporine (green) to induce apoptosis, or DMSO controls (pink), using the NucView® 488 and MitoView™ 633 Apoptosis Kit. Fluorescence was analyzed on a BD FACSCalibur flow cytometer. As apoptosis progresses over time in staurosporine-treated cells, NucView® 488 signal (FL1, x-axis) increases and mitochondrial membrane potential measured by MitoView™ 633 staining (FL4, y-axis) decreases.
MitoView™ Mitochondrial Dyes - Image 9Jurkat cells were treated with CCCP for 10 minutes to depolzarize mitocondria, or with staurosporine for 5 hours to induce apoptosis. Cells were stained for 30 minutes at 37°C with 150 nM MitoView™ 633, then washed and analyzed by flow cytometry in the FL4 channel of a BD FACSCalibur™ flow cytometer. Bars represent geometric mean fluorescence of the cell populations.
MitoView™ Mitochondrial Dyes - Image 10Click to view GIF animation. HeLa cells were stained with NucView® 488 Caspase-3 Substrate (2 uM), MitoView™ 633 mitochondrial potential dye (50 nM), and Hoechst 33342 blue nuclear stain (1 ug/mL), then treated with 1 uM staurosporine to induce apoptosis.
MitoView™ Mitochondrial Dyes - Image 11
MitoView™ Mitochondrial Dyes - Image 12
MitoView™ Mitochondrial Dyes - Image 13
MitoView™ Mitochondrial Dyes - Image 14
MitoView™ Mitochondrial Dyes - Image 15

Product Description

MitoView™ Dyes are fluorogenic mitochondrial stains for live cells.

  • Rapidly stain mitochondria in live cells without washing
  • Bright, photostable, and non-toxic for long-term live cell imaging
  • Available in five colors with blue, green, far-red, or near-IR emission
  • Monitor mitochondrial membrane potential with MitoView™ 633
  • Potential-independent MitoView™ Green can also stain fixed cells
  • MitoView™ 720 is a unique near-IR mitochondrial dye

These dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence. Cells can be fixed before staining with MitoView™ Green only, the other MitoView™ Dyes are for use in live cells only. We also offer MitoView™ Fix 640, a far-red mitochondrial stain suitable for no-wash, long term staining in live cells that is well-retained after fixation, permeabilization, and subsequent immunofluorescence staining.

Note: For staining mitochondria in fixed cells or tissue sections, we recommend using one our Mitochondrial Marker Antibodies, available with a wide selection of bright and photostable CF® Dyes and other conjugations.

MitoView™ Dyes can also be used to stain mitochondria in yeast and the cell interior of bacteria (gram-positive and gram-negative). See our Cellular Stains Table for more information on how our dyes stain various organisms.

MitoView™ Dyes

ProductEx/EmDetection channelPotentiometric?SizeCat. No.
MitoView™ 405398/440 nmDAPINo†50 ug70070-T
20x50 ug70070
MitoView™ Green490/523 nmFITC, GFPNo50 ug70054-T
20x50 ug70054
MitoView™ 633622/648 nm*Cy®5, APC*Yes50 ug70055-T
20x50 ug70055
MitoView™ Fix 640648/670 nmCy®5, APCNo‡50 ug70082-50ug
10x50 ug70082
MitoView™ 650644/670 nmCy®5, APCNo50 ug70075-50ug
20x50 ug70075
MitoView™ 720720/758 nm**Cy®5, Cy®7**No†50 ug70068-T
20x50 ug70068
* MitoView™ 633 also has red fluorescence in the Cy®3 channel and is not recommended for use with other red dyes.
**While optimal for Cy®7 settings, MitoView™ 720 is bright enough to be imaged in the Cy®5 channel.
† Dyes localize to the cytoplasm after mitochondrial depolarization, but still retain fluorescence.
‡ Reacts covalently with mitochondrial proteins for staining that is retained after fixation and permeabilization.

Find the Right Dye for Your Application

MitoView™ 405

MitoView™ 405 is a blue fluorescent mitochondrial dye with absorbance/emission at 398/440 nm, suitable for detection by confocal microscopy or flow cytometry using settings for DAPI or Pacific Blue®. The dye is membrane permeable and becomes brightly fluorescent upon accumulation in the mitochondrial membrane. Mitochondrial localization is dependent on mitochondrial membrane potential; when membrane potential is disrupted the dye relocalizes to the cytoplasm, but still retains fluorescence. The dye is designed for use in live cells and is not fixable. MitoView™ 405 is a replacement for our original MitoView™ Blue*, and has improved photostability.

MitoView™ Green

MitoView™ Green is a green fluorescent mitochondrial dye with properties similar to those of MitoTracker® Green FM. The dye is non-fluorescent until it partitions into the mitochondrial membrane. The staining relies on mitochondrial mass, not on mitochondria membrane potential. Thus, the dye can be used to stain mitochondria in both live cells and fixed cells. MitoView™ Green may be somewhat membrane potential-dependent in yeast cells.

Note: For optimal staining of mitochondria in fixed cells or tissue sections, we recommends using one our Mitochondrial Marker Antibodies, available with a wide selection of bright and photostable CF® Dyes and other labels.

MitoView™ 633

MitoView™ 633 is a far-red fluorescent mitochondrial dye with absorbance/emission at 622/648 nm. The dye is membrane-permeant and becomes brightly fluorescent upon accumulation in the mitochondrial membrane. Staining is dependent on mitochondrial membrane potential, and can be used to monitor mitochondrial membrane potential in intact cells. The dye is designed for use in live cells, and is not fixable. Note: The optimal detection settings for MitoView™ 633 are the same as for Cy®5 and other far-red dyes. However, the dye also has visible red fluorescence and can be imaged using Cy®3 settings as well. As a consequence, it may not be possible to use the dye for two-color imaging with other red probes.

MitoView™ 650

MitoView™ 650 is a far-red fluorescent mitochondrial dye that is not dependent on mitochondrial membrane potential. The dye has excitation/emission at 644/670 nm for the Cy®5 channel. Unlike MitoView™ 633, it does not bleed into the visible red channel, and so can be combined with red probes for multicolor imaging. The dye fluorescence is not lost after mitochondrial depolarization or fixation, but localization becomes non-specific.

MitoView™ 720

MitoView™ 720 is a near-infrared mitochondrial dye with absorbance/emission at 720/758 nm. While optimally detected using Cy®7 settings, the dye is bright enough to be imaged in the Cy®5 channel, and can be combined with visible red fluorescent probes. Mitochondrial localization is dependent on mitochondrial membrane potential; when membrane potential is disrupted the dye relocalizes to the cytoplasm but still retains fluorescence.

More Mitochondrial Dyes

In addition to MitoView™ Dyes, Biotium also offers several classical dyes for measuring mitochondrial membrane potential. These include JC-1, a ratiometric dye that forms red aggregates in healthy cells, which are reduced to the green monomeric state when mitochondrial membrane potential is lost. We also offer TMRM and TMRE, two red fluorescent dyes that are used to quantitatively measure mitochondrial membrane potential using the Nernst equation.

To view our wide selection of other cellular stains, visit our Cellular Stains technology page or see our Cellular Stains Brochure.

*Note: MitoView™ Blue (70052) has been discontinued and replaced by the improved MitoView™ 405. The original MitoView™ Blue will be available for purchase as a special order while supplies last. Contact techsupport@biotium.com to inquire about availability.

Cy Dye is a registered trademark of Cytiva. MitoTracker is a registered trademark of Thermo Fisher Scientific.

Back to top

Product Attributes

Size
Trial size (50 ug), 20 x 50 ug
Dye
MitoView™ 405, MitoView™ Green, MitoView™ 633, MitoView™ 650, MitoView™ 720
Probe cellular localization
Mitochondria
For live or fixed cells
For live/intact cells
Assay type/options
No-wash staining, Real-time imaging
Cell permeability
Membrane permeant
Potential dependence
Mitochondrial potential dependent, Mitochondrial potential-independent
Apoptosis/viability marker
Mitochondrial potential
Fixation options
Fix before staining (formaldehyde)
Antibody application notes
Cells can be fixed with formaldehyde before staining with MitoView™ Green only, Mitochondrial localization of MitoView™ Blue and MitoView™ 720 is potential-dependent, MitoView™ 633 fluorescence is mitochondrial potential-dependent, MitoView™ Green staining is mitochondrial potential-independent
Colors
Blue, Green, Far-red, Near-infrared
Storage Conditions
Store at -10 to -35 °C, Protect from light

Documents, Protocols, SDS and COA

Citations

Bioz Powered by Bioz See more details on Bioz

FAQs

Organelle & Cytoskeleton Stains

The mechanism of binding for RedDot™ 1 and RedDot™ 2 to DNA has not been characterized. However, based on the dye structure, it may bind by a similar mechanism as DRAQ®5, which has been reported in the literature to be a concentration-dependent intercalator and minor groove binder.

DRAQ is a registered trademark of Biostatus, Ltd.

Mitochondrial dyes, including MitoView™ Mitochondrial Dyes, are positively charged and lipophilic. They passively diffuse across cellular membranes and are presumed to accumulate in the mitochondrial matrix due to the proton gradient in the mitochondria (for a detailed review, see Cytometry Part A79A: 405-425, 2011).

However, some dyes are still retained in mitochondria after depolarization. Our dye chemists hypothesize that this is because some of the dyes are more lipophilic than others. Once they accumulate in the mitochondria because of their charge, they are less likely to diffuse back into the cytoplasm due to their hydrophobicity, even after the proton gradient that attracted them is dissipated by mitochondrial depolarization. Probably they associate with the mitochondrial membranes instead.

The so-called potential-independent dyes like MitoView™ Green, MitoTracker® Green, and Nonyl Acridine Orange are much more hydrophobic than potential-responsive dyes like MitoView™ 633, Rhodamine 123, and JC-1. The former dyes are retained after mitochondrial depolarization, and can be used to measure mitochondrial mass independent of potential. However, it would be more accurate to call these dyes relatively potential-insensitive, rather than potential-independent, because mitochondrial potential still plays a role in their localization. These dyes have been reported to show some loss of signal upon depolarization (Cytometry 39(3):203-10, 2000).

There is another class of mitochondrial dyes that accumulate in mitochondria based on charge, but also have a reactive group that can covalently link the dye to protein targets within the mitochondria, allowing them to be well-retained after fixation and permeabilization. Our MitoView™ Fix 640  is this type of dye.

Some dyes, like MitoView™ Green can stain mitochondria in cells that are already fixed. The mechanism by which this occurs is not well-understood. After fixation, there should be no proton gradient in the mitochondria to attract the dyes at all. Our chemists suspect that there may be some residual membrane potential in fixed mitochondria that is not due to the proton gradient (which would disappear following fixation), but instead arises from uneven distribution of proteins that have different isoelectric points (net charge). There are reports that the net charge of resident proteins in organelles differs based on the pH of the cellular compartment (Proc Natl Acad Sci USA 115(46):11778-11783, 2018). Charge differences may be sufficient to attract cationic lipophilic dyes to mitochondria in the absence of a proton gradient, due to a combination of weak electrostatic and hydrophobic interactions with mitochondrial proteins and membranes.

However, currently there is no direct evidence to suggest this is the mechanism for MitoView™ Green staining of fixed cells. There may be other targets that the dye is binding. For example, Nonyl Acridine Orange is reported to bind cardiolipin, a lipid that is enriched in mitochondrial membranes. It’s possible that MitoView™ Green binds to particular molecules in mitochondria with some degree of specificity. However, staining of fixed cells with mitochondrial dyes generally is not as specific as staining of live cells. That’s why we recommend using mitochondrial marker antibodies instead of dyes to stain fixed cells when possible.

Mounting medium can alter the staining of lipophilic dyes like LipidSpot™, due to interaction of the dyes with glycerol or other components that help form the interface between the coverslip and slide. The antifade compounds in mounting medium are generally compatible with the dyes. In our tests, LipidSpot™ staining was well preserved in EverBrite™ Mounting Medium (catalog. nos. 23001/23002) for up to 24 hours after mounting, but lipid droplet size and staining intensity were somewhat altered after samples were stored in mounting medium for several days. Therefore, if mounting medium is required to image samples, we’d recommend imaging as soon as possible after mounting.

LipidSpot™ is not compatible with FluoroShield mounting medium (staining is lost immediately after mounting). We have not tested other types of mounting medium.

View more FAQs

Can’t find your answer?

Contact Us

Related Products

New Products

Antibodies, Conjugates, & Labeling
Cellular Stains & Assays
Molecular Biology
Protein Detection & Analysis
Enzyme Substrates & Assays
Reagents & Accessories by Research Area
Reagents & Accessories by Application

Content #1

Content #1

Content #1

Content #2

Content #3