Primary & Secondary Antibody Conjugates

Biotium ships all antibodies (primary, secondary and conjugates) at room temperature. We guarantee their quality and performance under these conditions based upon our stability testing. Antibodies were subjected to accelerated stability testing by storing them at various temperatures (4°C, room temperature, or 37°C) for 1 week to mimic simulated shipping conditions and tested in immunostaining experiments. All antibodies showed the expected brightness and specificity, even after storage at sub-optimal temperatures for a week or longer. You can also download our Product Storage Statement here.

In line with our goal to be more environmentally friendly by reducing the use of excess packaging, and lowering shipping costs for our customers, products that have passed our stability testing are shipped at room temperature.

Once you have received the antibody vial, please follow the long-term storage instructions on the product information (PI) sheet.

Unless specified as isotype-specific, anti-IgG secondary antibodies are raised against whole immunoglobulin, so they are expected to cross-react widely with isotypes other than IgG.

We recommend using highly cross-adsorbed secondary antibodies whenever you perform multiple staining with two primary antibodies from different hosts. It is particularly important to use highly cross-adsorbed antibodies when staining with antibodies from two closely related species, such as mouse and rat.

Using highly cross-adsorbed secondary antibodies when staining tissues from a species with primary antibodies from a closely related species (for example, staining rat tissue with mouse primary antibody), will prevent background from cross-reactivity of the secondary with endogenous immunoglobulin in the tissue. This is important when staining immune tissues, but background from endogenous immunoglobulin is common in other tissues around blood vessels, where immunoglobulin also is abundant.