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Goat Anti-Mouse IgG (H+L)

This product is prepared by labeling high quality goat anti-mouse IgG (H+L) with a selection of our bright and photostable CF™ dyes and other labels.

Placeholder

Secondary antibody formats:
Liquid format:

  • CF™ dye and biotin conjugates: 2 mg/mL in PBS, 50% glycerol, 2 mg/ml BSA, 0.05% sodium azide
  • HRP conjugates: 1 mg/mL in PBS, 50% glycerol, 15 mg/ml BSA
  • Alkaline phosphatase conjugates: 1 mg/mL in PBS, 40% glycerol, 5 mg/ml BSA, 0.05% sodium azide
  • R-PE and APC conjugates: 0.5 mg/mL in PBS, 2 mg/ml
    BSA, 0.05% sodium azide

Lyophilized format (after reconstitution):

  • CF™ dye and biotin conjugates: 2 mg/mL in PBS, 15 mg/mL BSA, 20 mg/mL trehalose
  • HRP conjugates: 1 mg/mL PBS, 10 mg/mL trehalose
Catalog #

20464-1mL, 20306-1mg, 20306-1, 20197-1mg, 20197-1, 20183-1mg, 20183-1, 20180-1mg, 20180-1, 20140-1mg, 20140-1, 20120-1mg, 20120-1, 20110-1mg, 20352-1mL, 20352-200uL, 20365-500uL, 20464-100uL, 20411-100uL, 20411-0.5mL, 20408-50uL, 20408-500uL, 20408-1mg, 20400-1mL, 20400-1mg, 20400-100uL, 20386-50uL, 20386-500uL, 20386-1mg, 20365-50uL, 20110-1, 20100-1mg, 20100-1, 20183, 20180, 20140, 20120, 20110, 20100, 20080, 20070, 20054, 20050, 20040, 20030, 20010, 20378-50uL, 20197, 20306, 20080-1mg, 20080-1, 20070-1mg, 20070-1, 20054-1mg, 20054-1, 20050-1mg, 20050-1, 20040-1mg, 20040-1, 20030-1mg, 20030-1, 20010-1mg, 20010-1, - 20365-1mgView allHide

Antibody type

Secondary

Cross adsorption

Not cross-adsorbed

Host species

Goat

Reactivity (target)

Mouse IgG

Applications

Immunofluorescence, Immunohistology (formalin), Western, Immunohistology (frozen)

Conjugation
Size
Catalog #
price
Qty
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100 uL
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Taxes: Tax is assessed in the state of California and is calculated during checkout based on your shipping information. Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout.

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Product Description

This product is prepared by labeling high quality goat anti-mouse IgG (H+L) with a selection of fluorescent CF™ dyes and other labels.
CF™ dyes offer exceptional brightness and photostability. See the CF™ Dye Selection Guide under Downloads for more information.

Note: Conjugates of blue fluorescent dyes like CF™350, CF™405S, and CF™405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Goat Anti-Mouse IgG (H+L): Product & SKU Summary

ProductConjugationEx/EmSizeSKUPurchase
Alkaline Phosphatase Goat Anti-Mouse IgG (H+L)Alkaline PhosphataseN/A100 μL20464-100uLPurchase 20464-100uL
Alkaline Phosphatase Goat Anti-Mouse IgG (H+L)Alkaline PhosphataseN/A1 mL20464-1mLPurchase 20464-1mL
Biotin Goat Anti-Mouse IgG (H+L)BiotinN/A0.5 mL20183Purchase 20183
Biotin Goat Anti-Mouse IgG (H+L)BiotinN/A50 μL20183-1Purchase 20183-1
Biotin Goat Anti-Mouse IgG (H+L)BiotinN/A1 mg20183-1mgPurchase 20183-1mg
HRP Goat Anti-Mouse IgG (H+L)HRPN/A100 μL20400-100uLPurchase 20400-100uL
HRP Goat Anti-Mouse IgG (H+L)HRPN/A1 mL20400-1mLPurchase 20400-1mL
HRP Goat Anti-Mouse IgG (H+L)HRPN/A1 mg20400-1mgPurchase 20400-1mg
CF350 Goat Anti-Mouse IgG (H+L)CF350347/448 nm1 mg20140-1mgPurchase 20140-1mg
CF350 Goat Anti-Mouse IgG (H+L)CF350347/448 nm50 μL20140-1Purchase 20140-1
CF350 Goat Anti-Mouse IgG (H+L)CF350347/448 nm0.5 mL20140Purchase 20140
CF405S Goat Anti-Mouse IgG (H+L)CF405S404/431 nm50 μL20080-1Purchase 20080-1
CF405S Goat Anti-Mouse IgG (H+L)CF405S404/431 nm1 mg20080-1mgPurchase 20080-1mg
CF405S Goat Anti-Mouse IgG (H+L)CF405S404/431 nm0.5 mL20080Purchase 20080
CF405M Goat Anti-Mouse IgG (H+L)CF405M408/452 nm1 mg20180-1mgPurchase 20180-1mg
CF405M Goat Anti-Mouse IgG (H+L)CF405M408/452 nm50 μL20180-1Purchase 20180-1
CF405M Goat Anti-Mouse IgG (H+L)CF405M408/452 nm0.5 mL20180Purchase 20180
CF405L Goat Anti-Mouse IgG (H+L)CF405L395/545 nm1 mg20408-1mgPurchase 20408-1mg
CF405L Goat Anti-Mouse IgG (H+L)CF405L395/545 nm500 μL20408-500uLPurchase 20408-500uL
CF405L Goat Anti-Mouse IgG (H+L)CF405L395/545 nm50 μL20408-50uLPurchase 20408-50uL
CF488A Goat Anti-Mouse IgG (H+L)CF488A490/515 nm0.5 mL20010Purchase 20010
CF488A Goat Anti-Mouse IgG (H+L)CF488A490/515 nm50 μL20010-1Purchase 20010-1
CF488A Goat Anti-Mouse IgG (H+L)CF488A490/515 nm1 mg20010-1mgPurchase 20010-1mg
CF514 Goat Anti-Mouse IgG (H+L)CF514516/548 nm50 μL20386-50uLPurchase 20386-50uL
CF514 Goat Anti-Mouse IgG (H+L)CF514516/548 nm0.5 mL20386-500uLPurchase 20386-500uL
CF514 Goat Anti-Mouse IgG (H+L)CF514516/548 nm1 mg20386-1mgPurchase 20386-1mg
CF532 Goat Anti-Mouse IgG (H+L)CF532527/558 nm1 mg20365-1mgPurchase 20365-1mg
CF532 Goat Anti-Mouse IgG (H+L)CF532527/558 nm50 μL20365-50uLPurchase 20365-50uL
CF532 Goat Anti-Mouse IgG (H+L)CF532527/558 nm0.5 mL20365-500uLPurchase 20365-500uL
CF543 Goat Anti-Mouse IgG (H+L)CF543541/560 nm1 mg20306-1mgPurchase 20306-1mg
CF543 Goat Anti-Mouse IgG (H+L)CF543541/560 nm0.5 mL20306Purchase 20306
CF543 Goat Anti-Mouse IgG (H+L)CF543541/560 nm50 μL20306-1Purchase 20306-1
CF555 Goat Anti-Mouse IgG (H+L)CF555555/565 nm50 μL20030-1Purchase 20030-1
CF555 Goat Anti-Mouse IgG (H+L)CF555555/565 nm1 mg20030-1mgPurchase 20030-1mg
CF555 Goat Anti-Mouse IgG (H+L)CF555555/565 nm0.5 mL20030Purchase 20030
CF568 Goat Anti-Mouse IgG (H+L)CF568562/583 nm0.5 mL20100Purchase 20100
CF568 Goat Anti-Mouse IgG (H+L)CF568562/583 nm50 μL20100-1Purchase 20100-1
CF568 Goat Anti-Mouse IgG (H+L)CF568562/583 nm1 mg20100-1mgPurchase 20100-1mg
CF594 Goat Anti-Mouse IgG (H+L)CF594593/614 nm50 μL20110-1Purchase 20110-1
CF594 Goat Anti-Mouse IgG (H+L)CF594593/614 nm1 mg20110-1mgPurchase 20110-1mg
CF594 Goat Anti-Mouse IgG (H+L)CF594593/614 nm0.5 mL20110Purchase 20110
CF633 Goat Anti-Mouse IgG (H+L)CF633630/650 nm50 μL20120-1Purchase 20120-1
CF633 Goat Anti-Mouse IgG (H+L)CF633630/650 nm0.5 mL20120Purchase 20120
CF633 Goat Anti-Mouse IgG (H+L)CF633630/650 nm1 mg20120-1mgPurchase 20120-1mg
CF640R Goat Anti-Mouse IgG (H+L)CF640R642/662 nm1 mg20197-1mgPurchase 20197-1mg
CF640R Goat Anti-Mouse IgG (H+L)CF640R642/662 nm50 μL20197-1Purchase 20197-1
CF640R Goat Anti-Mouse IgG (H+L)CF640R642/662 nm0.5 mL20197Purchase 20197
CF647 Goat Anti-Mouse IgG (H+L)CF647650/665 nm1 mg20040-1mgPurchase 20040-1mg
CF647 Goat Anti-Mouse IgG (H+L)CF647650/665 nm50 μL20040-1Purchase 20040-1
CF647 Goat Anti-Mouse IgG (H+L)CF647650/665 nm0.5 mL20040Purchase 20040
CF660R Goat Anti-Mouse IgG (H+L)CF660R663/682 nm0.5 mL20054Purchase 20054
CF660R Goat Anti-Mouse IgG (H+L)CF660R663/682 nm1 mg20054-1mgPurchase 20054-1mg
CF660R Goat Anti-Mouse IgG (H+L)CF660R663/682 nm50 μL20054-1Purchase 20054-1
CF660C Goat Anti-Mouse IgG (H+L)CF660C667/685 nm50 μL20050-1Purchase 20050-1
CF660C Goat Anti-Mouse IgG (H+L)CF660C667/685 nm0.5 mL20050Purchase 20050
CF660C Goat Anti-Mouse IgG (H+L)CF660C667/685 nm1 mg20050-1mgPurchase 20050-1mg
CF750 Goat Anti-Mouse IgG (H+L)CF750755/777 nm1 mg20070-1mgPurchase 20070-1mg
CF750 Goat Anti-Mouse IgG (H+L)CF750755/777 nm50 μL20070-1Purchase 20070-1
CF750 Goat Anti-Mouse IgG (H+L)CF750755/777 nm0.5 mL20070Purchase 20070
CF790 Goat Anti-Mouse IgG (H+L)CF790784/806 nm50 μL20378-50uLPurchase 20378-50uL
R-PE Goat Anti-Mouse IgG (H+L)R-PE496, 546, 565/578 nm1 mL20352-1mLPurchase 20352-1mL
R-PE Goat Anti-Mouse IgG (H+L)R-PE496, 546, 565/578 nm200 μL20352-200uLPurchase 20352-200uL
APC Goat Anti-Mouse IgG (H+L)APC650/660 nm0.5 mL20411-0.5mLPurchase 20411-0.5mL
APC Goat Anti-Mouse IgG (H+L)APC650/660 nm100 μL20411-100uLPurchase 20411-100uL

References

Download a list of CF dye references.

 

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Support & Faq

Product shelf life, solubility, shipping and storage, and stability

Bioscience kits
The guaranteed shelf life from date of receipt for bioscience kits is listed on the product information sheet. Some kits have an expiration date printed on the kit box label, this is the guaranteed shelf life date calculated from the day that the product shipped from our facility. Kits often are functional for significantly longer than the guaranteed shelf life. If you have an older kit in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the kit still works for your application before processing a large number of samples or precious samples.

Antibodies and other conjugates
The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. If you have an older conjugate in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.

For lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates).

Chemicals, dyes, and gel stains
Biotium guarantees the stability of chemicals, dyes, and gel stains for at least a year from the date you receive the product. However, the majority of these products are highly stable for many years, as long as they are stored as recommended. Storage conditions can be found on the product information sheet or product safety and data sheet, material safety data sheet, and on the product label. Fluorescent compounds should be protected from light for long term storage.

If you have a Biotium compound that has been in storage for longer than one year that you wish to use, we recommend performing a small scale positive control experiment to confirm that the compound still works for your application before processing a large number of samples or precious samples.

Expiration date based on date of manufacture (DOM)
If your institution requires you to document expiration date based on date of manufacture for reagents, please contact techsupport@biotium.com for assistance.

Chemical products with special stability considerations:

Esters

Ester compounds include the following:
• Succinimidyl esters (SE, also known as NHS esters), such as our amine-reactive dyes
• Acetoxymethyl esters (AM esters) such as our membrane-permeable ion indicator dyes
• Diacetate-modified dyes, like ViaFluor™ 405, CFDA, and CFDA-SE cell viability/cell proliferation dyes

Ester dyes are stable in solid form as long as they are protected from light and moisture. Esters are not stable in aqueous solution. Concentrated stock solutions should be prepared in anhydrous DMSO (see Biotium catalog no. 90082). Stock solutions in anhydrous DMSO can be stored desiccated at -20°C for one month or longer. Esters should be diluted in aqueous solution immediately before use. Succinimidyl esters (SE) should be dissolved in a solution that is free of amine-containing compounds like Tris, glycine, or protein, which will react with the SE functional group. AM esters and diacetate compounds should be dissolved in a solution that is free of serum, because serum could contain esterases that would hydrolyze the compound.

A note on CF™ dye succinimidyl ester stability
Succinimidyl esters are generally susceptible to hydrolysis, which can result in lower labeling efficiency. Heavily sulfonated dyes, such as the Alexa Fluor® dyes, DyLight® dyes and IRDyes® are particularly hygroscopic, worsening the hydrolysis problem. For example, the percent of active Alexa Fluor® 488 succinimidyl ester (SE) could be well below 50% by the time of application (according to the manufacturer’s product datasheet). In a number of Alexa Fluor® SE reactive dyes, the SE group is derived from an aromatic carboxylic acid, while in all of Biotium’s CF™ dyes the SE group is prepared from an aliphatic carboxylic acid. This structural difference reduces the susceptibility of CF™ dye SE reactive groups to hydrolysis, resulting in relatively stable reactive dyes with consistently higher labeling efficiency compared to other SE derivatives of other fluorescent dyes.

Maleimides, MTS and thiosulfate dyes
Like the succinimidyl ester dyes, these dyes are also susceptible to hydrolysis, although generally to a much lower degree. Thus, for long term storage, anhydrous DMSO is recommended for making stock solutions.

Other reactive dyes
Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react with the dye’s functional group. See the product information sheets for specific reactive dyes more information.

Coelenterazines and D-luciferin

Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. Propylene glycol also can be used as a solvent to minimize evaporation. If the solvent evaporates, the coelenterazine will still be present in the vial, so note the volume in the vial prior to storage so that you can adjust the solvent volume to correct for evaporation if needed. Prepare working solutions in aqueous buffers immediately before use. Coelenterazines are stable for up to five hours in aqueous solution.

Aquaphile™ coelenterazines are water soluble formulations of coelenterazines. They are stable in solid form when stored as recommended. Aquaphile™ coelenterazines should be dissolved in aqueous solution immediately before use. They are stable for up to five hours in aqueous solution.

Note that coelenterazines are predominantly yellow solids, but may contain dark red or brown flecks. This does not affect product stability or performance. If your coelenterazine is uniformly brown, then it is oxidized and needs to be replaced.

D-luciferin is stable in solid form and as a concentrated stock solution when stored as recommended; it is not stable at dilute working concentrations in aqueous solution. Prepare concentrated D-luciferin stock solutions (typically 1-100 mg/mL) in water, and store in aliquots at -20°C or below for six months or longer. Prepare working solutions immediately before use.

Category: Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView™ Caspase 3 Enzyme Substrates, Luciferase Assays, CF™ Dyes, Product shelf life, solubility, shipping and storage, and stability

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Many of our solid compounds are packaged by lyophilization, in which case they usually do not appear as fluffy powders, but form a film or coating on the sides of the vial. Simply add the appropriate volume of the recommended solvent to the vial to make the desired concentration stock solution, and swirl or gently vortex to mix. Make sure the solvent comes in contact with the inside walls of the vial to fully recover the product.

Category: Product shelf life, solubility, shipping and storage, and stability

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Most of our products are stable at room temperature for many days, but we recommend storage at 4°C or -20°C to prolong shelf life. In the case of many of our aqueous dye solutions, the compounds are very stable at room temperature, but we recommend cold storage to prevent the growth of mold or other microbes over time. Therefore, to save on shipping costs, products with recommended storage at 4°C or -20°C may ship at ambient temperature without affecting product performance. When you receive the product, place it under the recommended storage conditions.

Category: Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView™ Caspase 3 Enzyme Substrates, Luciferase Assays, CF™ Dyes, Product shelf life, solubility, shipping and storage, and stability

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.