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Goat Anti-Rabbit IgG (H+L)

This product is prepared by labeling high quality goat anti-rabbit IgG (H+L) with a selection of our bright and photostable CF® dyes and other labels.

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Secondary antibody formats:
Liquid format:

  • CF® dye and biotin conjugates: 2 mg/mL in PBS, 50% glycerol, 2 mg/ml BSA, 0.05% sodium azide
  • HRP conjugates: 1 mg/mL in PBS, 50% glycerol, 15 mg/ml BSA
  • Alkaline phosphatase conjugates: 1 mg/mL in PBS, 40% glycerol, 5 mg/ml BSA, 0.05% sodium azide
  • R-PE and APC conjugates: 0.5 mg/mL in PBS, 2 mg/ml
    BSA, 0.05% sodium azide

Lyophilized format (after reconstitution):

  • CF® dye and biotin conjugates: 2 mg/mL in PBS, 15 mg/mL BSA, 20 mg/mL trehalose
  • HRP conjugates: 1 mg/mL PBS, 10 mg/mL trehalose
Catalog #

20465-1mL, 20353-1mL, 20309-1mg, 20309-1, 20202-1mg, 20202-1, 20185-1mg, 20185-1, 20181-1mg, 20181-1, 20141-1mg, 20141-1, 20122-1mg, 20122-1, 20112-1mg, 20353-200uL, 20366-1mg, 20465-100uL, 20412-500uL, 20412-100uL, 20409-50uL, 20409-500uL, 20409-1mg, 20402-1mL, 20402-1mg, 20402-100uL, 20387-50uL, 20387-500uL, 20387-1mg, 20366-50uL, 20366-500uL, 20112-1, 20102-1mg, 20202, 20185, 20181, 20141, 20122, 20112, 20102, 20082, 20073, 20055, 20053, 20043, 20033, 20012, 20309, 20012-1, 20102-1, 20082-1mg, 20082-1, 20073-1mg, 20073-1, 20055-1mg, 20055-1, 20053-1mg, 20053-1, 20043-1mg, 20043-1, 20033-1mg, 20033-1, 20012-1mg, - 20379-50uLView allHide

Antibody type

Secondary

Clonality

Polyclonal

Host species

Goat

Reactivity (target)

Rabbit IgG

Species reactivity

Rabbit

Cross adsorption

Not cross-adsorbed

Conjugation

Alkaline Phosphatase, APC, Biotin, CF350, CF405L, CF405M, CF405S, CF488A, CF514, CF532, CF543, CF555, CF568, CF594, CF633, CF640R, CF647, CF660C, CF660R, CF750, CF790, HRP, R-PE

Size

50 uL, 100 uL, 200uL, 500 uL, 0.5 mL, 1 mL, 1 mg

Applications

Immunofluorescence, Immunohistology (formalin), Western, Immunohistology (frozen)

Conjugation
Size
Catalog #
price
Qty
50 uL
100 uL
200uL
500 uL
0.5 mL
1 mL
1 mg
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Taxes: Tax is assessed in the state of California and is calculated during checkout based on your shipping information. Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout.

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Product Description

This product is prepared by labeling high quality goat anti-rabbit IgG (H+L) with a selection of fluorescent CF® dyes and other labels.
CF® dyes offer exceptional brightness and photostability. See the CF® Dye Selection Guide under Downloads for more information.

Note: Conjugates of blue fluorescent dyes like CF®350, CF®405S, and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Goat Anti-Rabbit IgG (H+L): Product & SKU Summary

ProductConjugationEx/EmSizeSKUPurchase
Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)Alkaline PhosphataseN/A100 μL20465-100uLPurchase 20465-100uL
Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)Alkaline PhosphataseN/A1 mL20465-1mLPurchase 20465-1mL
Biotin Goat Anti-Rabbit IgG (H+L)BiotinN/A0.5 mL20185Purchase 20185
Biotin Goat Anti-Rabbit IgG (H+L)BiotinN/A50 μL20185-1Purchase 20185-1
Biotin Goat Anti-Rabbit IgG (H+L)BiotinN/A1 mg20185-1mgPurchase 20185-1mg
HRP Goat Anti-Rabbit IgG (H+L)HRPN/A100 μL20402-100uLPurchase 20402-100uL
HRP Goat Anti-Rabbit IgG (H+L)HRPN/A1 mL20402-1mLPurchase 20402-1mL
HRP Goat Anti-Rabbit IgG (H+L)HRPN/A1 mg20402-1mgPurchase 20402-1mg
CF®350 Goat Anti-Rabbit IgG (H+L)CF®350347/448 nm0.5 mL20141Purchase 20141
CF®350 Goat Anti-Rabbit IgG (H+L)CF®350347/448 nm50 μL20141-1Purchase 20141-1
CF®350 Goat Anti-Rabbit IgG (H+L)CF®350347/448 nm1 mg20141-1mgPurchase 20141-1mg
CF®405L Goat Anti-Rabbit IgG (H+L)CF®405L395/545 nm1 mg20409-1mgPurchase 20409-1mg
CF®405L Goat Anti-Rabbit IgG (H+L)CF®405L395/545 nm500 μL20409-500uLPurchase 20409-500uL
CF®405L Goat Anti-Rabbit IgG (H+L)CF®405L395/545 nm50 μL20409-50uLPurchase 20409-50uL
CF®405M Goat Anti-Rabbit IgG (H+L)CF®405M408/452 nm0.5 mL20181Purchase 20181
CF®405M Goat Anti-Rabbit IgG (H+L)CF®405M408/452 nm50 μL20181-1Purchase 20181-1
CF®405M Goat Anti-Rabbit IgG (H+L)CF®405M408/452 nm1 mg20181-1mgPurchase 20181-1mg
CF®405S Goat Anti-Rabbit IgG (H+L)CF®405S404/431 nm0.5 mL20082Purchase 20082
CF®405S Goat Anti-Rabbit IgG (H+L)CF®405S404/431 nm50 μL20082-1Purchase 20082-1
CF®405S Goat Anti-Rabbit IgG (H+L)CF®405S404/431 nm1 mg20082-1mgPurchase 20082-1mg
CF®488A Goat Anti-Rabbit IgG (H+L)CF®488A490/515 nm0.5 mL20012Purchase 20012
CF®488A Goat Anti-Rabbit IgG (H+L)CF®488A490/515 nm50 μL20012-1Purchase 20012-1
CF®488A Goat Anti-Rabbit IgG (H+L)CF®488A490/515 nm1 mg20012-1mgPurchase 20012-1mg
CF®514 Goat Anti-Rabbit IgG (H+L)CF®514516/548 nm1 mg20387-1mgPurchase 20387-1mg
CF®514 Goat Anti-Rabbit IgG (H+L)CF®514516/548 nm0.5 mL20387-500uLPurchase 20387-500uL
CF®514 Goat Anti-Rabbit IgG (H+L)CF®514516/548 nm50 μL20387-50uLPurchase 20387-50uL
CF®532 Goat Anti-Rabbit IgG (H+L)CF®532527/558 nm1 mg20366-1mgPurchase 20366-1mg
CF®532 Goat Anti-Rabbit IgG (H+L)CF®532527/558 nm0.5 mL20366-500uLPurchase 20366-500uL
CF®532 Goat Anti-Rabbit IgG (H+L)CF®532527/558 nm50 μL20366-50uLPurchase 20366-50uL
CF®543 Goat Anti-Rabbit IgG (H+L)CF®543541/560 nm0.5 mL20309Purchase 20309
CF®543 Goat Anti-Rabbit IgG (H+L)CF®543541/560 nm50 μL20309-1Purchase 20309-1
CF®543 Goat Anti-Rabbit IgG (H+L)CF®543541/560 nm1 mg20309-1mgPurchase 20309-1mg
CF®555 Goat Anti-Rabbit IgG (H+L)CF®555555/565 nm0.5 mL20033Purchase 20033
CF®555 Goat Anti-Rabbit IgG (H+L)CF®555555/565 nm50 μL20033-1Purchase 20033-1
CF®555 Goat Anti-Rabbit IgG (H+L)CF®555555/565 nm1 mg20033-1mgPurchase 20033-1mg
CF®568 Goat Anti-Rabbit IgG (H+L)CF®568562/583 nm0.5 mL20102Purchase 20102
CF®568 Goat Anti-Rabbit IgG (H+L)CF®568562/583 nm50 μL20102-1Purchase 20102-1
CF®568 Goat Anti-Rabbit IgG (H+L)CF®568562/583 nm1 mg20102-1mgPurchase 20102-1mg
CF®594 Goat Anti-Rabbit IgG (H+L)CF®594593/614 nm0.5 mL20112Purchase 20112
CF®594 Goat Anti-Rabbit IgG (H+L)CF®594593/614 nm50 μL20112-1Purchase 20112-1
CF®594 Goat Anti-Rabbit IgG (H+L)CF®594593/614 nm1 mg20112-1mgPurchase 20112-1mg
CF®633 Goat Anti-Rabbit IgG (H+L)CF®633630/650 nm0.5 mL20122Purchase 20122
CF®633 Goat Anti-Rabbit IgG (H+L)CF®633630/650 nm50 μL20122-1Purchase 20122-1
CF®633 Goat Anti-Rabbit IgG (H+L)CF®633630/650 nm1 mg20122-1mgPurchase 20122-1mg
CF®640R Goat Anti-Rabbit IgG (H+L)CF®640R642/662 nm0.5 mL20202Purchase 20202
CF®640R Goat Anti-Rabbit IgG (H+L)CF®640R642/662 nm50 μL20202-1Purchase 20202-1
CF®640R Goat Anti-Rabbit IgG (H+L)CF®640R642/662 nm1 mg20202-1mgPurchase 20202-1mg
CF®647 Goat Anti-Rabbit IgG (H+L)CF®647650/665 nm0.5 mL20043Purchase 20043
CF®647 Goat Anti-Rabbit IgG (H+L)CF®647650/665 nm50 μL20043-1Purchase 20043-1
CF®647 Goat Anti-Rabbit IgG (H+L)CF®647650/665 nm1 mg20043-1mgPurchase 20043-1mg
CF®660C Goat Anti-Rabbit IgG (H+L)CF®660C667/685 nm0.5 mL20053Purchase 20053
CF®660C Goat Anti-Rabbit IgG (H+L)CF®660C667/685 nm50 μL20053-1Purchase 20053-1
CF®660C Goat Anti-Rabbit IgG (H+L)CF®660C667/685 nm1 mg20053-1mgPurchase 20053-1mg
CF®660R Goat Anti-Rabbit IgG (H+L)CF®660R663/682 nm0.5 mL20055Purchase 20055
CF®660R Goat Anti-Rabbit IgG (H+L)CF®660R663/682 nm50 μL20055-1Purchase 20055-1
CF®660R Goat Anti-Rabbit IgG (H+L)CF®660R663/682 nm1 mg20055-1mgPurchase 20055-1mg
CF®750 Goat Anti-Rabbit IgG (H+L)CF®750755/777 nm0.5 mL20073Purchase 20073
CF®750 Goat Anti-Rabbit IgG (H+L)CF®750755/777 nm50 μL20073-1Purchase 20073-1
CF®750 Goat Anti-Rabbit IgG (H+L)CF®750755/777 nm1 mg20073-1mgPurchase 20073-1mg
CF®790 Goat Anti-Rabbit IgG (H+L)CF®790784/806 nm50 μL20379-50uLPurchase 20379-50uL
R-PE Goat Anti-Rabbit IgG (H+L)R-PE496, 546, 565/578 nm1 mL20353-1mLPurchase 20353-1mL
R-PE Goat Anti-Rabbit IgG (H+L)R-PE496, 546, 565/578 nm200 μL20353-200uLPurchase 20353-200uL
APC Goat Anti-Rabbit IgG (H+L)APC650/660 nm100 μL20412-100uLPurchase 20412-100uL
APC Goat Anti-Rabbit IgG (H+L)APC650/660 nm500 μL20412-500uLPurchase 20412-500uL

References

Download a list of CF® dye references.

 

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Support & Faq

Product shelf life, solubility, shipping and storage, and stability

Bioscience kits
The guaranteed shelf life from date of receipt for bioscience kits is listed on the product information sheet. Some kits have an expiration date printed on the kit box label, this is the guaranteed shelf life date calculated from the day that the product shipped from our facility. Kits often are functional for significantly longer than the guaranteed shelf life. If you have an older kit in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the kit still works for your application before processing a large number of samples or precious samples.

Antibodies and other conjugates
The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. If you have an older conjugate in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.

For lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates).

Chemicals, dyes, and gel stains
Biotium guarantees the stability of chemicals, dyes, and gel stains for at least a year from the date you receive the product. However, the majority of these products are highly stable for many years, as long as they are stored as recommended. Storage conditions can be found on the product information sheet or product safety and data sheet, material safety data sheet, and on the product label. Fluorescent compounds should be protected from light for long term storage.

If you have a Biotium compound that has been in storage for longer than one year that you wish to use, we recommend performing a small scale positive control experiment to confirm that the compound still works for your application before processing a large number of samples or precious samples.

Expiration date based on date of manufacture (DOM)
If your institution requires you to document expiration date based on date of manufacture for reagents, please contact techsupport@biotium.com for assistance.

Chemical products with special stability considerations:

Esters

Ester compounds include the following:
• Succinimidyl esters (SE, also known as NHS esters), such as our amine-reactive dyes
• Acetoxymethyl esters (AM esters) such as our membrane-permeable ion indicator dyes
• Diacetate-modified dyes, like ViaFluor™ 405, CFDA, and CFDA-SE cell viability/cell proliferation dyes

Ester dyes are stable in solid form as long as they are protected from light and moisture. Esters are not stable in aqueous solution. Concentrated stock solutions should be prepared in anhydrous DMSO (see Biotium catalog no. 90082). Stock solutions in anhydrous DMSO can be stored desiccated at -20°C for one month or longer. Esters should be diluted in aqueous solution immediately before use. Succinimidyl esters (SE) should be dissolved in a solution that is free of amine-containing compounds like Tris, glycine, or protein, which will react with the SE functional group. AM esters and diacetate compounds should be dissolved in a solution that is free of serum, because serum could contain esterases that would hydrolyze the compound.

A note on CF™ dye succinimidyl ester stability
Succinimidyl esters are generally susceptible to hydrolysis, which can result in lower labeling efficiency. Heavily sulfonated dyes, such as the Alexa Fluor® dyes, DyLight® dyes and IRDyes® are particularly hygroscopic, worsening the hydrolysis problem. For example, the percent of active Alexa Fluor® 488 succinimidyl ester (SE) could be well below 50% by the time of application (according to the manufacturer’s product datasheet). In a number of Alexa Fluor® SE reactive dyes, the SE group is derived from an aromatic carboxylic acid, while in all of Biotium’s CF™ dyes the SE group is prepared from an aliphatic carboxylic acid. This structural difference reduces the susceptibility of CF™ dye SE reactive groups to hydrolysis, resulting in relatively stable reactive dyes with consistently higher labeling efficiency compared to other SE derivatives of other fluorescent dyes.

Maleimides, MTS and thiosulfate dyes
Like the succinimidyl ester dyes, these dyes are also susceptible to hydrolysis, although generally to a much lower degree. Thus, for long term storage, anhydrous DMSO is recommended for making stock solutions.

Other reactive dyes
Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react with the dye’s functional group. See the product information sheets for specific reactive dyes more information.

Coelenterazines and D-luciferin

Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. Propylene glycol also can be used as a solvent to minimize evaporation. If the solvent evaporates, the coelenterazine will still be present in the vial, so note the volume in the vial prior to storage so that you can adjust the solvent volume to correct for evaporation if needed. Prepare working solutions in aqueous buffers immediately before use. Coelenterazines are stable for up to five hours in aqueous solution.

Aquaphile™ coelenterazines are water soluble formulations of coelenterazines. They are stable in solid form when stored as recommended. Aquaphile™ coelenterazines should be dissolved in aqueous solution immediately before use. They are stable for up to five hours in aqueous solution.

Note that coelenterazines are predominantly yellow solids, but may contain dark red or brown flecks. This does not affect product stability or performance. If your coelenterazine is uniformly brown, then it is oxidized and needs to be replaced.

D-luciferin is stable in solid form and as a concentrated stock solution when stored as recommended; it is not stable at dilute working concentrations in aqueous solution. Prepare concentrated D-luciferin stock solutions (typically 1-100 mg/mL) in water, and store in aliquots at -20°C or below for six months or longer. Prepare working solutions immediately before use.

Category: Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView® Caspase 3 Enzyme Substrates, Luciferase Assays, CF® Dyes, Product shelf life, solubility, shipping and storage, and stability

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Many of our solid compounds are packaged by lyophilization, in which case they usually do not appear as fluffy powders, but form a film or coating on the sides of the vial. Simply add the appropriate volume of the recommended solvent to the vial to make the desired concentration stock solution, and swirl or gently vortex to mix. Make sure the solvent comes in contact with the inside walls of the vial to fully recover the product.

Category: Product shelf life, solubility, shipping and storage, and stability

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Most of our products are stable at room temperature for many days, but we recommend storage at 4°C or -20°C to prolong shelf life. In the case of many of our aqueous dye solutions, the compounds are very stable at room temperature, but we recommend cold storage to prevent the growth of mold or other microbes over time. Therefore, to save on shipping costs, products with recommended storage at 4°C or -20°C may ship at ambient temperature without affecting product performance. When you receive the product, place it under the recommended storage conditions.

Category: Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView® Caspase 3 Enzyme Substrates, Luciferase Assays, CF® Dyes, Product shelf life, solubility, shipping and storage, and stability

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.