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Goat Anti-Human IgG (H+L), Highly Cross-Adsorbed

Highly cross-adsorbed goat anti-human IgG (H L) secondary antibody labeled with our superior CF® Dyes.

Product Attributes

Antibody type

Secondary, Anti-Human Immunoglobulin

Clonality

Polyclonal

Host species

Goat

Antibody reactivity (target)

Human IgG

Species reactivity

Human

Cross adsorption

Bovine, Horse, Mouse

Concentration

2 mg/mL, 1 mg/mL (HRP, AP conjugates), 0.5 mg/mL (R-PE, APC conjugates)

Antibody/conjugate formulation

Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide, Lyophilized: PBS/15 mg/mL BSA/20 mg/mL trehalose after reconstitution, HRP conjugates: PBS/50% glycerol/15 mg/mL BSA, HRP conjugates (lyophilized): PBS/10 mg/mL trehalose after reconstitution

Secondary/tag antibody applications

ELISA, Flow cytometry, IHC, IF (cells or tissue sections), Western blot

Conjugation
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Catalog #
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50 uL
100 uL
200 uL
0.25 mL
0.5 mL
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1 mg
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Product Description

This is a highly cross-adsorbed goat anti-human IgG (H L) secondary antibody labeled with our bright and photostable CF® Dyes. To minimize cross-reactivity, the antibody has been adsorbed against bovine, horse,  and mouse serum.

  • Highly cross-adsorbed for specific staining with minimal background
  • Available in 11 bright and photostable CF® Dyes
  • HRP and R-PE conjugates also available
  • Suitable for western, immunofluorescence, and immunohistology in FFPE tissues

Goat Anti-Human IgG (H+L), Highly Cross-Adsorbed

ConjugationEx/EmSizeCatalog No.Dye Features
CF®488A490/515 nm50 uL (100 ug)20022-1CF®488A Features
0.5 mL (1 mg)20022
1 mg20022-1mg
CF®543541/560 nm50 uL (100 ug)20319-1CF®543 Features
0.5 mL (1 mg)20319
1 mg20319-1mg
CF®555555/565 nm50 uL (100 ug)20230-1CF®555 Features
0.5 mL (1 mg)20230
1 mg20230-1mg
CF®568562/583 nm50 uL (100 ug)20097-1CF®568 Features
0.5 mL (1 mg)20097
1 mg20097-1mg
CF®583R585/609 nm50 uL (100 ug)20901-50uLCF®583R Features
0.5 mL (1 mg)20901-500uL
CF®594593/614 nm50 uL (100 ug)20154-1CF®594 Features
0.5 mL (1 mg)20154
1 mg20154-1mg
CF®633630/650 nm50 uL (100 ug)20132-1CF®633 Features
0.5 mL (1 mg)20132
1 mg20132-1mg
CF®640R642/662 nm50 uL (100 ug)20081-1CF®640R Features
0.5 mL (1 mg)20081
1 mg20081-1mg
CF®647650/665 nm50 uL (100 ug)20280-1CF®647 Features
0.5 mL (1 mg)20280
1 mg20280-1mg
CF®680681/698 nm50 uL (100 ug)20287-1CF®680 Features
0.25 mL (500 ug)20287
CF®770770/797 nm50 uL (100 ug)20288-1CF®770 Features
0.25 mL (500 ug)20288
R-PE496, 546, 565/578 nm200 uL (100 ug)20355-200uL
1 mL (500 ug)20355-1mL
HRPN/A100 uL (100 ug)20470-100uL
1 mL (1 mg)20470-1mL
1 mg20470-1mg

View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of curated CF® Dye references.

 

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