CF® Dye Tyramides
Fluorescent CF® dye tyramide conjugates for tyramide signal amplification (TSA). A horseradish peroxidase (HRP)-mediated method for high-density labeling of a target protein or nucleic acid in situ.
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Product Description
CF® dye tyramide conjugates can be used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. A tyramide conjugate is incubated in the presence of HRP (often bound to a secondary antibody), which catalyzes the binding of the tyramide (and associated label) to adjacent tyrosines for enhanced fluorescent signal. Multiple rounds of tyramide signal amplification can be performed for multiplex detection; to learn more, see our Tech Tip: Multicolor Fluorescence Imaging using Tyramide Amplification Kits.
We also offer Ready-to-Use Tyramide Amplification Buffer, and CF® Dye Tyramide Amplification Kits.
Learn more about tyramide signal amplification.
CF Dye Tyramides: Product & SKU Summary
Product | Conjugation | Ex/Em | Size | SKU | Purchase |
---|---|---|---|---|---|
CF350® Tyramide | CF®350 | 347/448 nm | 0.5 mg | 92170 | Purchase 92170 |
CF®405L Tyramide | CF®405L | 395/545 nm | 0.5 mg | 92198 | Purchase 92198 |
CF®405S Tyramide | CF®405S | 404/431 nm | 0.5 mg | 92197 | Purchase 92197 |
CF®405M Tyramide | CF®405M | 408/452 nm | 0.5 mg | 96057 | Purchase 96057 |
CF®430 Tyramide | CF®430 | 426/498 nm | 0.5 mg | 96053 | Purchase 96053 |
CF®488A Tyramide | CF®488A | 490/515 nm | 0.5 mg | 92171 | Purchase 92171 |
CF®514 Tyramide | CF®514 | 516/548 nm | 0.5 mg | 92199 | Purchase 92199 |
CF®532 Tyramide | CF®532 | 527/558 nm | 0.5 mg | 96066 | Purchase 96066 |
CF®543 Tyramide | CF®543 | 541/560 nm | 0.5 mg | 92172 | Purchase 92172 |
CF®555 Tyramide | CF®555 | 555/565 nm | 0.5 mg | 96021 | Purchase 96021 |
CF®568 Tyramide | CF®568 | 562/583 nm | 0.5 mg | 92173 | Purchase 92173 |
CF®594 Tyramide | CF®594 | 593/614 nm | 0.5 mg | 92174 | Purchase 92174 |
CF®620R Tyramide | CF®620R | 617/639 nm | 0.5 mg | 92194 | Purchase 92194 |
CF®640R Tyramide | CF®640R | 642/662 nm | 0.5 mg | 92175 | Purchase 92175 |
CF®647 Tyramide | CF®647 | 650/665 nm | 0.5 mg | 96022 | Purchase 96022 |
CF®660R Tyramide | CF®660R | 663/682 nm | 0.5 mg | 92195 | Purchase 92195 |
CF®680R Tyramide | CF®680R | 680/701 nm | 0.5 mg | 92196 | Purchase 92196 |
CF®750 Tyramide | CF®750 | 755/777 nm | 0.5 mg | 96052 | Purchase 96052 |
References
1. Journal of Immunological Methods 125, 279-285 (1989).
2. Journal of Immunological Methods 137, 103-112 (1991).
3. Cytometry A. (2010), doi:10.1002/cyto.a.20970
4. Molecular Neurodegeneration (2017), doi: 10.1186/s13024-017-0202-z (CF568® Tyramide)
Download a list of CF® dye references.