CF®405S Dye

CF®405S is a blue fluorescent dye with an absorption peak wavelength matching the 405 nm blue diode laser line (Figure 1). In addition, the emission peak wavelength of the dye is well-centered within the blue detection window of BD flow cytometers. As a result, flow cytometry analysis using CF®405S dye results in much brighter signal than that using Alexa Fluor® 405 (Figure 2). CF®405S also has been validated in super-resolution imaging by SIM.

See products available with CF®405S. Additional conjugates or reactive dyes may be added upon request; contact techsupport@biotium.com to inquire.

Figure 1. Normalized spectra of CF®405S goat anti-mouse conjugate in PBS.

CF®405S Technical Summary

Abs/Em maxima: 404/431 nm
Extinction coefficient: 33,000
Molecular weight: ~1,169
Excitation laser line: 405 nm
Direct replacement for: Alexa Fluor® 405, Cascade Blue®, DyLight® 405
Fluorescence lifetime:
τ (ns)/free acid in PBS pH 7.4, ε (ns) = 3.88 ± 0.05

Visit our FAQ for more fluorescence life time information on CF® dyes.

Figure 2. CF®405S yields brighter conjugates than Alexa Fluor® 405. Flow cytometry analysis in the Pacific Blue® channel of a BD LSRII of Jurkat cells stained w... See More

MCF-7 cells stained with rabbit anti-COXIV, followed by CF®405S goat anti-rabbit (mitochondria, blue) and mouse anti-pPol-II, followed by CF®647 goat anti-mouse... See More

Helpful Tools & Resources

Spectra Viewer

Visualize and export the fluorescent dye spectrum of any CF® Dye or other dyes from Biotium.

Download Our CF® Dye eBook

Learn the history of fluorophores in life science research as well as the development and advantages of CF® Dyes.

CF® Dye Selection Tool

Check out our handy fluorophore selection tool to help choose the best CF® Dye for your next experiment.

CF® Dye Reference Guide

Download our single page reference guide for all CF® Dye fluorophores. Includes recommended channels, applications, relative brightness, and photostability.