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Fluorescent CF® Dyes CF®568 Dye

CF®568 Dye

Outshines Alexa Fluor® 568
  • Much brighter conjugates than Alexa Fluor® 568
  • Excellent photostability for demanding imaging applications
  • Compatible SIM, TIRF, and multicolor STORM
CF® Dye Spectrum:
CF®350 Dye
CF®350 is a blue fluorescent dye
CF®405S Dye
Improved brightness & photostability for the 405 nm laser line
CF®405M Dye
Improved brightness & photostability for the 405 nm laser line
CF®430 & CF®440 Dyes
Photostable 405 nm-excitable dyes with green fluorescence
CF®405L Dye
A 405 nm-excitable dye with green fluorescence emission
CF®450 Dye
A spectrally unique, 405 nm-excitable green dye
CF®488A Dye
A superior green fluorescent dye
CF®503R Dye
A spectrally unique green dye ideal for spectral flow cytometry
CF®514 Dye
Alternative green fluorescent dye
CF®532 Dye
A bright green fluorescent dye for the 532 nm laser
CF®543 Dye
An orange fluorescent dye ideal for the 543 nm laser
CF®535ST Dye
A red fluorescent dye designed for STORM super-resolution imaging
CF®550R Dye
A spectrally unique orange/red dye ideal for spectral flow cytometry
CF®555 Dye
A bright and photostable orange-red dye
CF®568 Dye
Outshines Alexa Fluor®568
CF®570
Bright red fluorescent dye
CF®583 & CF®583R Dyes
Bright red fluorescent dyes
CF®594 Dye
Truly the brightest deep red dye
CF®597R Dye
Unique green-excited dye for STORM
RPE-Astral™616
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®620R Dye
A bright and photostable far-red dye
CF®633 Dye
The best dye for 633/635 laser lines
CF®640R Dye
A highly photostable far-red dye
CF®647 Dye
A highly fluorescent far-red dye
CF®660C & CF®660R
Superior alternatives to Alexa Fluor® 660
CF®680 & CF®680R
Two outstanding 680 nm-excitable dyes
CF®700 Dye
Brighter alternative to Alexa Fluor® 700
CF®710 Dye
Near-infrared CF® Dye with exceptional photostability
CF®725 Dye
Near-infrared CF® Dye with exceptional photostability
CF®740 Dye
Near-infrared CF® Dye with exceptional photostability
CF®750 Dye
Near-infrared CF® Dye
CF®770 Dye
Near-infrared CF® Dye
CF®790 Dye
Near-infrared CF® Dye
APC-Astral™813
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®800 Dye
Near-infrared CF® Dye
CF®820 Dye
Near-infrared CF® Dye
CF®850 Dye
Near-infrared CF® Dye
CF®870 Dye
Near-infrared CF® Dye

CF®568 is a red fluorescent dye with excitation optimally matching the 568 nm laser line. Its antibody conjugates are significantly brighter and more photostable than Alexa Fluor® 568, making it ideal for confocal and single molecule imaging. CF®568 has been validated for super-resolution techniques including SIM, TIRF, STED, and multicolor STORM, and produces better STORM images than Cy®3b (learn more about CF® Dyes for Super-Resolution Imaging).

See products available with CF®568. Additional conjugates or reactive dyes may be added upon request; contact techsupport@biotium.com to inquire.

Figure 1. Normalized absorption and emission spectra of CF®568 goat anti-mouse antibody conjugate in PBS.

CF®568 Technical Summary

  • Abs/Em maxima: 562/584 nm
  • Extinction coefficient: 100,000
  • Molecular weight: ~714
  • Excitation laser line: 532 nm or 568 nm
  • Direct replacement for: Alexa Fluor® 568, ATTO 565, Rhodamine Red
  • Fluorescence lifetime:
  • τ (ns)/free acid in PBS pH 7.4, ε (ns) = 3.66 ± 0.05
  • τ (ns) /S.Ab§ = 1.539

Visit our FAQ for more fluorescence life time information on CF® Dyes.

Figure 2. CF®568 produces brighter antibody conjugates than Alexa Fluor® 568. Jurkat cells were stained with mouse anti-CD3 or isotype control, followed by CF®5... See More
Figure 3. CF®568 is more photostable than Alexa Fluor® 568. Jurkat cells were stained with biotinylated anti-CD3 antibody followed by CF®568 or Alexa Fluor®568 ... See More
Figure 4. MCF-7 cells stained with CF®568 monoclonal anti-Ep-CAM (EGP40/826) (red) and Hoechst (blue).
Figure 5. HeLa cells stained with NucSpot® 470 (green) and CF®568 phalloidin (red).
Figure 6. Section of rat intestine stained with CF®568 phallodin (red). Nuclei are stained with DAPI (blue).

Helpful Tools & Resources

Visualize and export the fluorescent dye spectrum of any CF® Dye or other dyes from Biotium.
Learn the history of fluorophores in life science research as well as the development and advantages of CF® Dyes.
Check out our handy fluorophore selection tool to help choose the best CF® Dye for your next experiment.
Download our single page reference guide for all CF® Dye fluorophores. Includes recommended channels, applications, relative brightness, and photostability.
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CF®555 Dye
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CF®570

CF is a registered trademark of Biotium, Inc; Alexa Fluor is a registered trademark of Thermo Fisher Scientific; CY DYE is a registered trademark of Cytiva.

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