CF®594 Dye

CF®594 is a deep-red fluorescent dye spectrally similar to Alexa Fluor® 594 and Texas Red® dye (Figure 1). It is significantly brighter than Alexa Fluor® 594 when conjugated to proteins, due to its high quantum yield and exceptional water solubility (Figure 2). CF®594 also is very photostable (Figure 3), making it ideal for demanding applications such as confocal microscopy. Its emission is well-separated from green dyes like CF®488A for multicolor imaging.

See products available with CF®594. Additional conjugates or reactive dyes may be added upon request; contact techsupport@biotium.com to inquire.

Figure 1. Normalized absorption and emission spectra of CF®594 goat anti-mouse IgG conjugate in PBS.

CF®594 Technical Summary

Abs/Em maxima: 593/614 nm
Extinction coefficient: 115,000
Molecular weight: ~730
Laser line: 561 to 568 nm
Direct replacement for: Alexa Fluor® 594, Texas Red®, DyLight® 594
Fluorescence lifetime:
τ (ns) /S.Ab§ = 1.746

Visit our FAQ for more fluorescence life time information on CF® Dyes.

Figure 2. CF®594 yields brighter conjugates than Alexa Fluor® 594. Relative fluorescence of goat anti-mouse IgG labeled with varying degrees of labeling (DOL, o... See More

Figure 3. Photostability of CF®594 and AlexaFluor®594. Jurkat cells were stained with mouse anti-CD3 and CF®594 or Alexa Fluor® 594 goat anti-mouse IgG. Cells w... See More

Figure 4. *S. cerevisiae* yeast stained with CF®594 wheat germ agglutinin (cell walls, red) and CF®488A concanavalin A (bud scars, green).

Figure 5. Neuromuscular junction in a section of rat skeletal muscle stained with CF®594 α-bungarotoxin (red). Nuclei are stained blue with DAPI.

Fixed oleic-acid treated HeLa cells stained with LipidSpot™ 488 (lipid droplets, green) CF®594 WGA (cell surface, red), and Hoechst 33258 (nuclei, blue).

Helpful Tools & Resources

Spectra Viewer

Visualize and export the fluorescent dye spectrum of any CF® Dye or other dyes from Biotium.

Download Our CF® Dye eBook

Learn the history of fluorophores in life science research as well as the development and advantages of CF® Dyes.

CF® Dye Selection Tool

Check out our handy fluorophore selection tool to help choose the best CF® Dye for your next experiment.

CF® Dye Reference Guide

Download our single page reference guide for all CF® Dye fluorophores. Includes recommended channels, applications, relative brightness, and photostability.