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Fluorescent CF® Dyes CF®633 Dye

CF®633 Dye

The best dye for 633/635 nm laser lines
  • Yields the brightest conjugates among similar dyes
  • Far more photostable than Alexa Fluor® 647
  • Validated in TIRF and other super-resolution methods
CF® Dye Spectrum:
CF®350 Dye
CF®350 is a blue fluorescent dye
CF®405S Dye
Improved brightness & photostability for the 405 nm laser line
CF®405M Dye
Improved brightness & photostability for the 405 nm laser line
CF®430 & CF®440 Dyes
Photostable 405 nm-excitable dyes with green fluorescence
CF®405L Dye
A 405 nm-excitable dye with green fluorescence emission
CF®450 Dye
A spectrally unique, 405 nm-excitable green dye
CF®488A Dye
A superior green fluorescent dye
CF®503R Dye
A spectrally unique green dye ideal for spectral flow cytometry
CF®514 Dye
Alternative green fluorescent dye
CF®532 Dye
A bright green fluorescent dye for the 532 nm laser
CF®543 Dye
An orange fluorescent dye ideal for the 543 nm laser
CF®535ST Dye
A red fluorescent dye designed for STORM super-resolution imaging
CF®550R Dye
A spectrally unique orange/red dye ideal for spectral flow cytometry
CF®555 Dye
A bright and photostable orange-red dye
CF®568 Dye
Outshines Alexa Fluor®568
CF®570
Bright red fluorescent dye
CF®583 & CF®583R Dyes
Bright red fluorescent dyes
CF®594 Dye
Truly the brightest deep red dye
CF®597R Dye
Unique green-excited dye for STORM
RPE-Astral™616
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®620R Dye
A bright and photostable far-red dye
CF®633 Dye
The best dye for 633/635 laser lines
CF®640R Dye
A highly photostable far-red dye
CF®647 Dye
A highly fluorescent far-red dye
CF®660C & CF®660R
Superior alternatives to Alexa Fluor® 660
CF®680 & CF®680R
Two outstanding 680 nm-excitable dyes
CF®700 Dye
Brighter alternative to Alexa Fluor® 700
CF®710 Dye
Near-infrared CF® Dye with exceptional photostability
CF®725 Dye
Near-infrared CF® Dye with exceptional photostability
CF®740 Dye
Near-infrared CF® Dye with exceptional photostability
CF®750 Dye
Near-infrared CF® Dye
CF®770 Dye
Near-infrared CF® Dye
CF®790 Dye
Near-infrared CF® Dye
APC-Astral™813
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®800 Dye
Near-infrared CF® Dye
CF®820 Dye
Near-infrared CF® Dye
CF®850 Dye
Near-infrared CF® Dye
CF®870 Dye
Near-infrared CF® Dye

Far-red fluorescent dyes like Cy®5 have long been favored for their sensitivity in detecting minimal autofluorescence in biological samples. Recently, Alexa Fluor® 647 has emerged with brighter fluorescence and enhanced photostability, though it still falls short for some applications. Alexa Fluor® 633, while photostable, exhibits weak protein fluorescence. Addressing these challenges, Biotium introduces CF®633 with advanced chemistry, boasting significantly brighter fluorescence and unparalleled photostability (Figure 2). This makes CF®633 ideal for systems using 633 or 635 nm laser excitation, including TIRF and super-resolution imaging (learn more about CF® Dyes for Super-Resolution Imaging).

See products available with CF®633. Additional reactive dyes or conjugates may be added on request, contact techsupport@biotium.com to inquire.

CF®633 Features

  • Brightest far-red fluorescent conjugates
  • Excellent photostability
  • Highly water soluble and pH-insensitive
  • Compatible with TIRF and other super-resolution methods

CF®633 Technical Summary

  • Abs/Em maxima: 630/650 nm
  • Extinction coefficient: 100,000
  • Molecular weight: ~820
  • Laser line: 633 or 635 nm
  • Alternative for: Alexa Fluor® 633, Alexa Fluor® 647, Cy®5 and DyLight® 649
  • Fluorescence lifetime:
    τ (ns)/free acid in PBS pH 7.4, ε (ns) = 3.39* (in water)
    τ (ns) /S.Ab§ = 3
    Lifetime data obtained via customer communication under different experimental conditions and imaging setup.

Visit our FAQ for more fluorescence lifetime information on CF® Dyes.

Figure 1. Normalized spectra of CF®633 goat anti-mouse conjugate in PBS.
Figure 2. CF®633 yields the brightest far-red conjugates. Jurkat cells were stained with mouse anti-CD3 or isotype control antibody, followed by goat anti-mouse... See More
Figure 3. CF®633 and CF®640R are much more photostable than Alexa Fluor® 647. Jurkat cells were stained with mouse anti-CD3 antibody followed by goat anti-mouse... See More
Figure 4. Relative photostability of CF®633 and Alexa Fluor® 647 fluorescence. Jurkat cells were stained with mouse anti-CD3 antibody followed by goat anti-mous... See More
Figure 5. HeLa cells stained with CF®633 phalloidin (actin filaments, magenta) and DAPI (nuclei, cyan).
Figure 6. Rat neuromuscular junction stained with CF®633 α-bungarotoxin (magenta) and DAPI (cyan).
Figure 7. S. cerevisiae yeast stained with CF®633 concanavalin A (ConA) (cell walls, magenta).

Helpful Tools & Resources

Visualize and export the fluorescent dye spectrum of any CF® Dye or other dyes from Biotium.
Learn the history of fluorophores in life science research as well as the development and advantages of CF® Dyes.
Check out our handy fluorophore selection tool to help choose the best CF® Dye for your next experiment.
Download our single page reference guide for all CF® Dye fluorophores. Includes recommended channels, applications, relative brightness, and photostability.
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CF®620R Dye
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CF®640R Dye

CF is a registered trademark of Biotium Inc. Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific; Cy Dye is a registered trademark of Cytiva.

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