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CF®680 and CF®680R are two outstanding near-IR dyes excited at 680 nm with emission near 700 nm. The dyes each have unique properties suited to different applications.
Both CF®680 and CF®680R are validated for super-resolution applications such as STORM and STED (learn more about CF® Dyes for super-resolution imaging).
See products available with CF®680 and CF®680R. Additional conjugates or reactive dyes may be added upon request; contact techsupport@biotium.com to inquire.
CF®680
CF®680 is a highly water-soluble cyanine-based dye. The dye is excellent for labeling antibodies, producing the brightest fluorescence for near-infrared western blotting (Figure 2). With its long wavelength emission, CF®680 is also suitable for near-infrared small animal imaging. However, because of its relatively large molecular size, CF®680 is not recommended for labeling nucleic acids or small biomolecules, for which our CF®680R is better suited.
CF®680R
CF®680R is a novel rhodamine dye that is highly fluorescent and extremely photostable. Biotium scientists developed a method to make bright, photostable, and water-soluble rhodamine dyes with red to near-IR wavelengths. These dyes are excellent for labeling proteins, nucleic acids, and small biomolecules, and are ideal for confocal microscopy, single molecule imaging, and other applications requiring brightness and photostability.
CF®680 Technical Summary
- Abs/Em maxima: 681/698 nm
- Extinction coefficient: 210,000
- Molecular weight: ~3241
- Laser line: 680 or 685 nm
- Alternative for: Alexa Fluor® 680, Cy®5.5, DyLight®680, IRDye®680LT
- Fluorescence lifetime:
τ (ns)/free acid in PBS pH 7.4, ε (ns) = 1.23 ± 0.05
τ (ns) /S.Ab§ = 1.277
CF®680R Technical Summary
- Abs/Em maxima: 680/701 nm
- Extinction coefficient: 140,000
- Molecular weight: ~912
- Laser line: 680 or 685 nm
- Alternative for: Alexa Fluor® 680, Cy®5.5, DyLight®680, IRDye®680LT
- Fluorescence lifetime:
τ (ns)/free acid in PBS pH 7.4, ε (ns) = 1.22 ± 0.05
τ (ns) /S.Ab§ = 1.6
Helpful Tools & Resources
Visualize and export the fluorescent dye spectrum of any CF® Dye or other dyes from Biotium.
Learn the history of fluorophores in life science research as well as the development and advantages of CF® Dyes.
Check out our handy fluorophore selection tool to help choose the best CF® Dye for your next experiment.
Download our single page reference guide for all CF® Dye fluorophores. Includes recommended channels, applications, relative brightness, and photostability.
CF is a registered trademark of Biotium, Inc. Alexa Fluor is a registered trademark of Thermo Fisher Scientific; Cy Dye is a registered trademark of Cytiva; IRDye and Odyssey are registered trademarks of LI-COR Biosciences.
