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CF®680 and CF®680R are two outstanding near-IR dyes excited at 680 nm with emission near 700 nm. The dyes each have unique properties suited to different applications.
CF®680 is a highly water-soluble cyanine-based dye with a molecular weight of ~3000. This dye is excellent for labeling antibodies, producing the brightest fluorescence for near-infrared western blotting (Figure 2). With its long wavelength emission, CF®680 is also suitable for near-infrared small animal imaging. However, because of its relatively large molecular size, CF®680 is not recommended for labeling nucleic acids or small biomolecules, for which our CF®680R is better suited. CF®680 is validated in multicolor STORM and 3D super-resolution imaging (learn more about CF® dyes for super-resolution imaging).
CF®680R is a novel rhodamine-based dye with a relatively small molecular weight of ~912. The dye is highly fluorescent and, more importantly, extremely photostable (Figure 3). Rhodamine dyes are traditionally known to be bright and photostable. However, it has been a synthetic challenge to make functional rhodamine dyes with long wavelengths and high water solubility necessary for bioconjugation. Scientists at Biotium have overcome the challenge and invented a new way to make highly water soluble, bright and photostable rhodamine dyes with wavelengths ranging from red to near-IR. These dyes are excellent for labeling proteins, nucleic acids and small bio-molecules. They are ideal for confocal microscopy, single molecule imaging and other applications that demand both brightness and photostability. CF®680R is validated in STED super-resolution imaging and single molecule spectroscopy (see more CF® dyes for super-resolution microscopy).
CF®680 Technical Summary
- Abs/Em maxima: 681/698 nm
- Extinction coefficient: 210,000
- Molecular weight: ~3241
- Laser line: 680 or 685 nm
- Alternative for: Alexa Fluor® 680, Cy®5.5, DyLight®680, IRDye®680LT
- Fluorescence lifetime:
τ (ns)/free acid in PBS pH 7.4, ε (ns) = 1.23 ± 0.05
τ (ns) /S.Ab§ = 1.277
CF®680R Technical Summary
- Abs/Em maxima: 680/701 nm
- Extinction coefficient: 140,000
- Molecular weight: ~912
- Laser line: 680 or 685 nm
- Alternative for: Alexa Fluor® 680, Cy®5.5, DyLight®680, IRDye®680LT
- Fluorescence lifetime:
τ (ns)/free acid in PBS pH 7.4, ε (ns) = 1.22 ± 0.05
τ (ns) /S.Ab§ = 1.6
CF is a registered trademark of Biotium, Inc. Alexa Fluor is a registered trademark of Thermo Fisher Scientific; Cy Dye is a registered trademark of Cytiva; IRDye and Odyssey are registered trademarks of LI-COR Biosciences.
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