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CF® Dyes

Next-Generation Fluorescent Probes Designed for Life Science Research

High-Performance CF® Dyes

Superior brightness, photostability, and solubility

Multi-Color Flexibility

Over 40 CF® Dyes across the visible, far-red & near-IR spectra

Unrivaled Near-IR Dyes

Ideal for near-IR western or small animal imaging

Super-Resolution Microscopy

The best performance for STORM, SIM, and other super-resolution imaging methods

Rapid & Simple Antibody Labeling

CF® Dyes are available with Biotium's super-simple and versatile Mix-n-Stain™ Antibody Labeling Kits

Wide Selection of Products

CF® Dyes are available as primary and secondary antibodies, conjugates, reactive dyes, and labeling kits
CF® Dye Spectrum:
CF®350 Dye
CF®350 is a blue fluorescent dye
CF®405S Dye
Improved brightness & photostability for the 405 nm laser line
CF®405M Dye
Improved brightness & photostability for the 405 nm laser line
CF®430 & CF®440 Dyes
Photostable 405 nm-excitable dyes with green fluorescence
CF®405L Dye
A 405 nm-excitable dye with green fluorescence emission
CF®450 Dye
A spectrally unique, 405 nm-excitable green dye
CF®488A Dye
A superior green fluorescent dye
CF®503R Dye
A spectrally unique green dye ideal for spectral flow cytometry
CF®514 Dye
Alternative green fluorescent dye
CF®532 Dye
A bright green fluorescent dye for the 532 nm laser
CF®543 Dye
An orange fluorescent dye ideal for the 543 nm laser
CF®535ST Dye
A red fluorescent dye designed for STORM super-resolution imaging
CF®550R Dye
A spectrally unique orange/red dye ideal for spectral flow cytometry
CF®555 Dye
A bright and photostable orange-red dye
CF®568 Dye
Outshines Alexa Fluor®568
CF®570
Bright red fluorescent dye
CF®583 & CF®583R Dyes
Bright red fluorescent dyes
CF®594 Dye
Truly the brightest deep red dye
CF®597R Dye
Unique green-excited dye for STORM
RPE-Astral™616
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®620R Dye
A bright and photostable far-red dye
CF®633 Dye
The best dye for 633/635 laser lines
CF®640R Dye
A highly photostable far-red dye
CF®647 Dye
A highly fluorescent far-red dye
CF®660C & CF®660R
Superior alternatives to Alexa Fluor® 660
CF®680 & CF®680R
Two outstanding 680 nm-excitable dyes
CF®700 Dye
Brighter alternative to Alexa Fluor® 700
CF®710 Dye
Near-infrared CF® Dye with exceptional photostability
CF®725 Dye
Near-infrared CF® Dye with exceptional photostability
CF®740 Dye
Near-infrared CF® Dye with exceptional photostability
CF®750 Dye
Near-infrared CF® Dye
CF®770 Dye
Near-infrared CF® Dye
CF®790 Dye
Near-infrared CF® Dye
APC-Astral™813
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®800 Dye
Near-infrared CF® Dye
CF®820 Dye
Near-infrared CF® Dye
CF®850 Dye
Near-infrared CF® Dye
CF®870 Dye
Near-infrared CF® Dye

Achieve Clearer Fluorescent Results with CF® Dyes

CF® Dyes are a series of highly water-soluble fluorescent dyes spanning the visible and near-infrared spectrum for labeling biomolecules such as proteins and nucleic acids. Developed in house by our industry-leading chemists, CF® Dyes offer superior brightness, photostability, and signal-to-noise when compared to other commercially available fluorescent dyes. Watch our video below to learn how CF® Dyes can offer clearer fluorescent resultsd

Choose the Best CF® Dye for Your Next Experiment

The CF® Dye Selection Tool will recommend the most optimal CF® Dye for a given laser/filter combination.
Check out our handy CF® Dye Selection Tool to find the optimal CF® Dye for a specific laser/filter combination for microscopy, flow cytometry, or western blotting.

Learn More About CF® Dyes in Our eBook

In this eBook, you will learn more about:

  • The history of fluorescent dyes in life science research
  • The development and advantages of CF® Dyes
  • Using CF® Dyes for specialized applications such as super-resolution microscopy or spectral flow cytometry

Product Spotlight

Resources That Fuel Bright Ideas

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Related Solutions

Primary Antibodies

Access a growing catalog of 2000+ monoclonal antibodies conjugated to bright CF® Dyes. Includes validated options for IF, IHC, and Western Blot.

CF® Dye-Labeled Secondary Antibodies

Choose from a wide selection of highly specific and versatile fluorescent secondary antibodies conjugated to your choice of 20+ bright and photostable CF® Dyes

Mix-n-Stain™ Antibody Labeling Kits

Simplify and accelerate covalent antibody labeling with flexible dye, enzyme, and protein tag options—always stable, reliable, and high-performing.

FAQs

CF® Dyes are ideal for protein labeling because of their high water solubility, which reduces fluorescence quenching. They are also useful for labeling oligonucleotides that require multiple copies of a dye for maximal fluorescence, such as the preparation of FISH probes, where water soluble dyes can minimize fluorescence quenching. Finally, CF® Dyes make excellent polar tracers that can be used for visualizing the morphology or long-term tracing of neurons after microinjection. CF® Dyes and their conjugates are ideal for fluorescence microscopy applications, flow cytometry, Western blotting, and in vivo imaging (near-IR CF® Dyes).

CF® Dyes are highly water soluble (>100 mg/mL). They are also very soluble in other polar solvents, such as DMSO, DMF, methanol and ethanol. However, CF® Dyes are poorly soluble or insoluble in non-polar solvents.

Most of our products are stable at room temperature for many days, so in all likelihood the product will still work just fine. To be on the safe side, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.

One exception that we are aware of is GelGreen®, which is more sensitive to light exposure than most of our other fluorescent dyes. If GelGreen® is exposed to ambient light for a prolonged period of time (days to weeks), its color will change from dark orange to brick red. If this occurs, the GelGreen will no longer work for gel staining.

 

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