Astral Leap™ Offers Exceptional Tandem Dye Performance
Astral Leap™ dyes are novel long Stokes shift tandem dyes that enable expanded multiplexing for flow cytometry with exceptional signal-to-noise. Astral Leap™ tandem dyes optimize both the Förster Resonance Energy Transfer (FRET) dye capabilities and conjugation to the antibody.
Astral Leap™ tandem dyes may be used for either conventional or spectral flow cytometry instruments. Their unique long Stokes shift spectral profiles are particularly useful for unmixing on spectral flow cytometry instruments such as the Cytek® Aurora. The tandem dyes are currently available with validated Biotium Choice primary antibodies. See below for our full selection of antibodies available with Astral Leap™ tandem dyes.
Learn more about our validated dyes for spectral flow cytometry and other reagents for flow cytometry.
RPE-Astral™616 Technical Summary
- Abs/Em maxima: 496, 546, 566/617 nm
- Laser line: 488, 532, and 561 nm
- Spectrally similar to: PE/Dazzle™ 594, PE-CF®594, and PE-DyLight® 594
APC-Astral™813 Technical Summary
- Abs/Em maxima: 633, 638/813 nm
- Laser line: 633 -640 nm
- Spectrally similar to: APC/Fire™ 813, cFluor®R840


RPE-Astral™775 Technical Summary
- Abs/Em maxima: 496, 546, 565/774 nm
- Laser line: 488, 532, and 561 nm
- Spectrally similar to: PE/Cy®7, PE/Cyanine 7

Antibodies labeled with Astral Leap™ tandem dyes utilize Biotium’s patented high-performance conjugation chemistry and are rigorously validated by flow cytometry.



Astral™ Tandem Dye Labeled Antibodies
| Biotium Choice Antibody | RPE-Astral™616 (496, 546, 566/617 nm) | RPE-Astral™775 (496, 546, 565/774 nm) | APC-Astral™813 (633, 638/813 nm) |
|---|---|---|---|
| CD3e Monoclonal Mouse (SK7) | P002-R616 | P002-R775 | |
| CD4 Monoclonal Mouse (SK3) | P001-R616 | P001-R775 | P001-A813 |
| CD8a Monoclonal Mouse (SK1) | P009-R616 | P009-R775 | P009-A813 |
| CD9 Monoclonal Mouse (HI9a) | P003S-R616 | P003S-A813 | |
| CD9 Recombinant Monoclonal Mouse (2310.9) | P015-R616 | P015-R775 | P015-A813 |
| CD16 Monoclonal Mouse (3G8) | P011-R616 | P011-A813 | |
| CD19 Monoclonal Mouse (SJ25C1) | P010-R616 | P010-A813 | |
| CD45 Monoclonal Mouse (2D1) | P012-R616 | P012-R775 | P012-A813 |
| rCD63 Monoclonal Mouse (2304.63) | P013-R775 | ||
| CD81 Monoclonal Mouse (5A6) | P005S-R616 | ||
| CD81 Recombinant Monoclonal Mouse (2304.81) | P014-R616 | P014-R775 |
Helpful Tools & Resources
CF is a registered trademark of Biotium, Inc; Cytek and cFluor are registered trademarks of Cytek, Inc; DyLight is a registered trademark of Thermo Fisher Scientific; CY DYE is a registered trademark of Cytiva; PE/Dazzle and PE/Fire are trademarks of Biolegend, Inc.



