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Fluorescent CF® Dyes Astral Leap™ Tandem Dyes

Astral Leap™ Tandem Dyes

Exceptionally Bright Long Stokes Shift Dyes to Maximize Your Flow Results
  • Expands Flow Cytometry Multiplexing
    Unique long Stokes shift tandem dyes for flow cytometry
  • Developed for Optimal Performance
    Utilizes breakthrough chemical engineering for maximum FRET and signal:noise
  • Available for Validated Primary Antibodies
    Flow tested antibodies for important immune and exosome targets
CF® Dye Spectrum:
CF®350 Dye
CF®350 is a blue fluorescent dye
CF®405S Dye
Improved brightness & photostability for the 405 nm laser line
CF®405M Dye
Improved brightness & photostability for the 405 nm laser line
CF®430 & CF®440 Dyes
Photostable 405 nm-excitable dyes with green fluorescence
CF®405L Dye
A 405 nm-excitable dye with green fluorescence emission
CF®450 Dye
A spectrally unique, 405 nm-excitable green dye
CF®488A Dye
A superior green fluorescent dye
CF®503R Dye
A spectrally unique green dye ideal for spectral flow cytometry
CF®514 Dye
Alternative green fluorescent dye
CF®532 Dye
A bright green fluorescent dye for the 532 nm laser
CF®543 Dye
An orange fluorescent dye ideal for the 543 nm laser
CF®535ST Dye
A red fluorescent dye designed for STORM super-resolution imaging
CF®550R Dye
A spectrally unique orange/red dye ideal for spectral flow cytometry
CF®555 Dye
A bright and photostable orange-red dye
CF®568 Dye
Outshines Alexa Fluor®568
CF®570
Bright red fluorescent dye
CF®583 & CF®583R Dyes
Bright red fluorescent dyes
CF®594 Dye
Truly the brightest deep red dye
CF®597R Dye
Unique green-excited dye for STORM
RPE-Astral™616
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®620R Dye
A bright and photostable far-red dye
CF®633 Dye
The best dye for 633/635 laser lines
CF®640R Dye
A highly photostable far-red dye
CF®647 Dye
A highly fluorescent far-red dye
CF®660C & CF®660R
Superior alternatives to Alexa Fluor® 660
CF®680 & CF®680R
Two outstanding 680 nm-excitable dyes
CF®700 Dye
Brighter alternative to Alexa Fluor® 700
CF®710 Dye
Near-infrared CF® Dye with exceptional photostability
CF®725 Dye
Near-infrared CF® Dye with exceptional photostability
CF®740 Dye
Near-infrared CF® Dye with exceptional photostability
CF®750 Dye
Near-infrared CF® Dye
CF®770 Dye
Near-infrared CF® Dye
CF®790 Dye
Near-infrared CF® Dye
APC-Astral™813
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®800 Dye
Near-infrared CF® Dye
CF®820 Dye
Near-infrared CF® Dye
CF®850 Dye
Near-infrared CF® Dye
CF®870 Dye
Near-infrared CF® Dye

Astral Leap™ Offers Exceptional Tandem Dye Performance

Astral Leap™ dyes are novel long Stokes shift tandem dyes that enable expanded multiplexing for flow cytometry with exceptional signal-to-noise. Astral Leap™ tandem dyes optimize both the Förster Resonance Energy Transfer (FRET) dye capabilities and conjugation to the antibody.

RPE-Astral™616 and APC-Astral™813 may be used for either conventional or spectral flow cytometry instruments. Their unique long Stokes shift spectral profiles are particularly useful for unmixing on spectral flow cytometry instruments such as the Cytek® Aurora. Both dyes are currently available with validated Biotium Choice primary antibodies. See below for our full selection of antibodies available with Astral Leap™ tandem dyes.

Learn more about our validated dyes for spectral flow cytometry and other reagents for flow cytometry.

RPE-Astral™616 Technical Summary

  • Abs/Em maxima: 496, 546, 566/617 nm
  • Laser line: 488, 532, and 561 nm
  • Spectrally similar to: PE/Dazzle™ 594, PE-CF®594, and PE-DyLight® 594

APC-Astral™813 Technical Summary

  • Abs/Em maxima: 633, 638/813 nm
  • Laser line: 633 -640 nm
  • Spectrally similar to: APC/Fire™ 813, cFluor®R840

Antibodies labeled with Astral Leap™ tandem dyes utilize Biotium’s patented high-performance conjugation chemistry and are rigorously validated by flow cytometry.

Flow cytometry of lymphocyte-gated PBMCs unstained (gray) or stained with CD4 Mouse Monoclonal Antibody (SK3), RPE-Astral™616 Conjugate (red). Staining was dete... See More
Flow cytometry of MCF-7 cells unstained (gray) or stained with CD9 Mouse Monoclonal Antibody (HI9a), APC-Astral™813 Conjugate (teal). Staining was detected in t... See More

Helpful Tools & Resources

Visualize and export the fluorescent dye spectrum of any CF® Dye or other dyes from Biotium.
Learn the history of fluorophores in life science research as well as the development and advantages of CF® Dyes.
Check out our handy fluorophore selection tool to help choose the best CF® Dye for your next experiment.
Download our single page reference guide for all CF® Dye fluorophores. Includes recommended channels, applications, relative brightness, and photostability.
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CF®597R Dye
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CF®620R Dye

CF is a registered trademark of Biotium, Inc; Cytek and cFluor are registered trademarks of Cytek, Inc; DyLight is a registered trademark of Thermo Fisher Scientific; PE/Dazzle and PE/Fire are trademarks of Biolegend, Inc.

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