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Fluorescent CF® Dyes CF®700

CF®700

  • Superior brightness over Alexa Fluor®700
  • Improved signal-to-noise for flow cytometry
  • Available in select primary antibodies, labeling kits, and reactive formats
CF® Dye Spectrum:
CF®350 Dye
CF®350 is a blue fluorescent dye
CF®405S Dye
Improved brightness & photostability for the 405 nm laser line
CF®405M Dye
Improved brightness & photostability for the 405 nm laser line
CF®430 & CF®440 Dyes
Photostable 405 nm-excitable dyes with green fluorescence
CF®405L Dye
A 405 nm-excitable dye with green fluorescence emission
CF®450 Dye
A spectrally unique, 405 nm-excitable green dye
CF®488A Dye
A superior green fluorescent dye
CF®503R Dye
A spectrally unique green dye ideal for spectral flow cytometry
CF®514 Dye
Alternative green fluorescent dye
CF®532 Dye
A bright green fluorescent dye for the 532 nm laser
CF®543 Dye
An orange fluorescent dye ideal for the 543 nm laser
CF®535ST Dye
A red fluorescent dye designed for STORM super-resolution imaging
CF®550R Dye
A spectrally unique orange/red dye ideal for spectral flow cytometry
CF®555 Dye
A bright and photostable orange-red dye
CF®568 Dye
Outshines Alexa Fluor®568
CF®570
Bright red fluorescent dye
CF®583 & CF®583R Dyes
Bright red fluorescent dyes
CF®594 Dye
Truly the brightest deep red dye
CF®597R Dye
Unique green-excited dye for STORM
RPE-Astral™616
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®620R Dye
A bright and photostable far-red dye
CF®633 Dye
The best dye for 633/635 laser lines
CF®640R Dye
A highly photostable far-red dye
CF®647 Dye
A highly fluorescent far-red dye
CF®660C & CF®660R
Superior alternatives to Alexa Fluor® 660
CF®680 & CF®680R
Two outstanding 680 nm-excitable dyes
CF®700 Dye
Brighter alternative to Alexa Fluor® 700
CF®710 Dye
Near-infrared CF® Dye with exceptional photostability
CF®725 Dye
Near-infrared CF® Dye with exceptional photostability
CF®740 Dye
Near-infrared CF® Dye with exceptional photostability
CF®750 Dye
Near-infrared CF® Dye
CF®770 Dye
Near-infrared CF® Dye
CF®790 Dye
Near-infrared CF® Dye
APC-Astral™813
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®800 Dye
Near-infrared CF® Dye
CF®820 Dye
Near-infrared CF® Dye
CF®850 Dye
Near-infrared CF® Dye
CF®870 Dye
Near-infrared CF® Dye

Near-IR CF®700 is Biotium’s next-generation dye with superior brightness and signal-to-noise. Unlike other near-IR dyes that suffer from aggregation and poor stability, CF®700 uses novel molecular engineering to deliver exceptional brightness. It outperforms Alexa Fluor® 700 in both microscopy and flow cytometry, offering a brighter signal and higher stain index (Figures 2–3). CF®700 is extensively validated and available in a range of formats, including Biotium Choice primary antibodies, Annexin V conjugates, Mix-n-Stain™ Antibody Labeling Kits, and more.

See products available with CF®700. Additional conjugates or reactive dyes may be added upon request; contact techsupport@biotium.com to inquire.

CF®700 Technical Summary

  • Abs/Em Maxima: 696/721 nm
  • Extinction coefficient: 240,000
  • Molecular weight: ~ 2315
  • Excitation laser line: 633, 635, 680 or 685 nm
  • Replaces: Alexa Fluor® 700, DyLight® 700-B1

CF®700 Features

  • Brighter near-IR alternative for Alexa Fluor® 700
  • Superior signal-to-noise for antibodies and other bioconjugates
  • Exceptional performance for microscopy and flow cytometry
Figure 1. Normalized absorbance and emission spectra of CF®700 goat anti-mouse conjugate in PBS.
Figure 2. PBMCs stained side by side with antibodies for the same target and of the same clone, conjugated either to CF®700 or to Alexa Fluor® 700. Cells were a... See More
Figure 3. PBMCs stained side by side with antibodies for the same target and of the same clone, conjugated either to CF®700 or to Alexa Fluor® 700. Cells were a... See More
Figure 4. PBMCs stained with CD3(SK7)-CF®700 and CD3(SK7)-Alexa Fluor® 700 (BioLegend® 344821) and analyzed in the R712 (APC-700) channel of a CytoFLEX® flow cy... See More
Figure 5. PBMCs stained with CD4(SK3)-CF®700 and CD4(SK3)-Alexa Fluor® 700 (BioLegend® 344621) and analyzed in the R712 (APC-700) channel of a CytoFLEX® flow cy... See More
Figure 6. PBMCs stained with CD8(SK1)-CF®700 and CD8(SK1)-Alexa Fluor® 700 (BioLegend® 344723) and analyzed in the R712 (APC-700) channel of a CytoFLEX® flow cy... See More
Figure 7. CD3- PBMCs stained with CD16(3G8)-CF®700 and CD16(3G8)-Alexa Fluor® 700 (BioLegend® 302007) and analyzed in the R712 (APC-700) channel of a CytoFLEX® ... See More
Figure 8. PBMCs stained with CD19(SJ25C1)-CF®700 and CD19(SJ25C1)-Alexa Fluor® 700 (BioLegend® 363033) and analyzed in the R712 (APC-700) channel of a CytoFLEX®... See More

Helpful Tools & Resources

Visualize and export the fluorescent dye spectrum of any CF® Dye or other dyes from Biotium.
Learn the history of fluorophores in life science research as well as the development and advantages of CF® Dyes.
Check out our handy fluorophore selection tool to help choose the best CF® Dye for your next experiment.
Download our single page reference guide for all CF® Dye fluorophores. Includes recommended channels, applications, relative brightness, and photostability.
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CF®680 & CF®680R
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CF®870 Dye

CF is a registered trademark of Biotium, Inc.; Alexa Fluor is a registered trademark of Thermo Fisher Scientific; BioLegend is a registered trademark of Biolegend, Inc; CytoFLEX is a registered trademark of Beckman Coulter, Inc.

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