Near-IR CF®700 is Biotium’s next-generation dye with superior brightness and signal-to-noise. Unlike other near-IR dyes that suffer from aggregation and poor stability, CF®700 uses novel molecular engineering to deliver exceptional brightness. It outperforms Alexa Fluor® 700 in both microscopy and flow cytometry, offering a brighter signal and higher stain index (Figures 2–3). CF®700 is extensively validated and available in a range of formats, including Biotium Choice primary antibodies, Annexin V conjugates, Mix-n-Stain™ Antibody Labeling Kits, and more.
Superior signal-to-noise for antibodies and other bioconjugates
Exceptional performance for microscopy and flow cytometry
Figure 1. Normalized absorbance and emission spectra of CF®700 goat anti-mouse conjugate in PBS.
Figure 2. PBMCs stained side by side with antibodies for the same target and of the same clone, conjugated either to CF®700 or to Alexa Fluor® 700. Cells were a... See More
Figure 3. PBMCs stained side by side with antibodies for the same target and of the same clone, conjugated either to CF®700 or to Alexa Fluor® 700. Cells were a... See More
Figure 4. PBMCs stained with CD3(SK7)-CF®700 and CD3(SK7)-Alexa Fluor® 700 (BioLegend® 344821) and analyzed in the R712 (APC-700) channel of a CytoFLEX® flow cy... See More
Figure 5. PBMCs stained with CD4(SK3)-CF®700 and CD4(SK3)-Alexa Fluor® 700 (BioLegend® 344621) and analyzed in the R712 (APC-700) channel of a CytoFLEX® flow cy... See More
Figure 6. PBMCs stained with CD8(SK1)-CF®700 and CD8(SK1)-Alexa Fluor® 700 (BioLegend® 344723) and analyzed in the R712 (APC-700) channel of a CytoFLEX® flow cy... See More
Figure 7. CD3- PBMCs stained with CD16(3G8)-CF®700 and CD16(3G8)-Alexa Fluor® 700 (BioLegend® 302007) and analyzed in the R712 (APC-700) channel of a CytoFLEX® ... See More
Figure 8. PBMCs stained with CD19(SJ25C1)-CF®700 and CD19(SJ25C1)-Alexa Fluor® 700 (BioLegend® 363033) and analyzed in the R712 (APC-700) channel of a CytoFLEX®... See More
Helpful Tools & Resources
Visualize and export the fluorescent dye spectrum of any CF® Dye or other dyes from Biotium.
Learn the history of fluorophores in life science research as well as the development and advantages of CF® Dyes.
Check out our handy fluorophore selection tool to help choose the best CF® Dye for your next experiment.
Download our single page reference guide for all CF® Dye fluorophores. Includes recommended channels, applications, relative brightness, and photostability.
CF is a registered trademark of Biotium, Inc.; Alexa Fluor is a registered trademark of Thermo Fisher Scientific; BioLegend is a registered trademark of Biolegend, Inc; CytoFLEX is a registered trademark of Beckman Coulter, Inc.
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