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Near-IR CF® Dyes are next-gen long wavelength dyes offering a major advance in brightness and stability over current near-IR dyes, which often suffer from aggregation and poor performance. Biotium’s novel molecular engineering overcomes these issues, creating exceptionally bright, photostable dyes.
A proprietary structural feature also improves water solubility without excessive charge, allowing higher dye-to-protein ratios while maintaining conjugate specificity and in vivo stability. Biotium offers six near-IR CF® Dyes, plus far-red/near-IR CF®680 and CF®680R, which are also compatible with NIR westerns and the LI-COR® Odyssey®.
Near-Infrared CF® Dyes
Advantages
- Superior brightness and photostability in the near-IR
- Near-IR conjugates with unrivaled signal-to-noise
- Spectrally unique dyes extending to 900 nm emission
- Ideal for multiplexing or in vivo imaging
- Wide selection of antibodies, bioconjugates, & other formats
Applications
- NIR western/In Cell Western®
- In vivo small animal imaging
- Flow cytometry & microscopy
- Super-resolution microscopy
- Photoacoustic imaging
Technical Summary
CF®700
- Abs/Em: 696/721 nm
- ε: 240,000
- Molecular weight: ~2315
- Laser line: 633 to 685 nm
- Replaces: Alexa Fluor® 700, DyLight® 700-B1
CF®710
- Abs/Em: 712/736 nm
- ε: 115,000
- Molecular weight: ~860
- Laser line: 633 to 685 nm
- Replaces: Alexa Fluor® 700
- Exceptional photostability
CF®725
- Abs/Em: 729/750 nm
- ε: 120,000
- Molecular weight: ~890
- Laser line: 633 to 685 nm
- Exceptional photostability
CF®740
- Abs/Em: 742/767 nm
- ε: 105,000
- Molecular weight: ~900
- Laser line: 633 to 685 nm
- Alternative for: Alexa Fluor® 750, Cy®7, DyLight® 750
- Superior chemical stability for TSA and photostability for microscopy
CF®750
- Abs/Em: 755/777 nm
- ε: 250,000
- Molecular weight: ~3009
- Laser line: 633 to 685 nm
- Alternative for: Alexa Fluor® 750, Cy®7, DyLight® 750
- Fluorescence life time: τ (ns)/free acid in PBS pH 7.4, ε (ns) = 0.58 ± 0.05 τ (ns) /S.Ab§ = 0.636
- Validated in STORM & photoacoustic imaging
CF®770
- Abs/Em: 770/797 nm
- ε: 220,000
- Molecular weight: ~3138
- Laser line: 785 nm
- Replaces: DyLight® 800, IRDye® 800CW
- Matches the LI-COR® Odyssey® 800 channel
CF®790
- Abs/Em: 784/806 nm
- ε: 210,000
- Molecular weight: ~3267
- Laser line: 785 nm
- Replaces: Alexa Fluor® 790
- Fluorescence life time:
- τ (ns)/free acid in PBS pH 7.4, ε (ns) = 0.39 ± 0.05
- τ (ns) /S.Ab§ = 0.54
CF®800
- Abs/Em: 797/816 nm
- ε: 210,000
- Molecular weight: ~3334
- Laser line: 785 nm
- Spectrally similar to: Indocyanine Green
CF®820
- Abs/Em: 822/835 nm
- ε: 253,000
- Molecular weight: ~2553
- Laser line: 785 nm
- Replaces: DY-820
CF®850
- Abs/Em: 852/870 nm
- ε: 240,000
- Molecular weight: ~2787
- Laser line: 808 nm
CF®870
- Abs/Em: 876/896 nm
- ε: 240,000
- Molecular weight: ~2773
- Laser line: 808 nm
CF is a registered trademark of Biotium, Inc. Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific; Cy Dye is a registered trademark of Cytiva; IRDye and Odyssey are registered trademarks of LI-COR Biosciences.
