CF®405M is a blue fluorescent dye spectrally similar to Pacific Blue® dye. CF®405M is as bright as Pacific Blue® dye but is significantly more photostable than the latter (Figures 2 and 3). An additional advantage of CF®405M is that it has less spillover in the 525/50 green channel than Pacific Blue® dye due to narrower emission peak, making CF®405M a superior choice in multi-color detection applications. CF®405M also has been validated in super-resolution imaging by SIM.
Figure 1. Normalized absorption and emission spectra of CF®405M goat anti-mouse IgG conjugate in PBS.
CF®405M Technical Summary
Abs/Em maxima: 408/452 nm
Extinction coefficient: 41,000
Molecular weight: ~503
Excitation laser line: 405 nm
Direct replacement for: Pacific Blue®, BD Horizon™ V450
Figure 2. CF®405M is as bright as Pacific Blue™ in flow cytometry. Jurkat cells were stained with intracellular CD3 followed by CF®405M or Pacific Blue™ goat an... See More
Figure 3. CF®405M is more photostable than Pacific Blue™. CF®405M and Pacific Blue™ solutions were continuously exposed to mercury arc lamp excitation with a DA... See More
Figure 4. Methanol-fixed MCF-7 cells stained with mouse anti-tubulin antibody plus CF®405M goat anti-mouse secondary antibody (microtubules, cyan) and RedDot™2 ... See More
Figure 5. MCF-7 cells stained with CF®405M monolconal mouse anti-Cyclin B1 antibody, clone CCNB1/1098 (nuclei/nucleoli, blue) and CF®488A phalloidin (actin fila... See More
Figure 6. HeLa cells stained with rabbit anti-COXIV + CF®488A goat anti-rabbit (mitochondria, green), mouse anti-Golgin 97 + CF®555 goat anti-mouse (Golgi, red)... See More
Helpful Tools & Resources
Visualize and export the fluorescent dye spectrum of any CF® Dye or other dyes from Biotium.
Learn the history of fluorophores in life science research as well as the development and advantages of CF® Dyes.
Check out our handy fluorophore selection tool to help choose the best CF® Dye for your next experiment.
Download our single page reference guide for all CF® Dye fluorophores. Includes recommended channels, applications, relative brightness, and photostability.
CF is a registered trademark of Biotium; Pacific Blue is a registered trademark of Thermo Fisher Scientific; BD Horizon is a trademark of BD Biosciences.
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