CF®597R is a deep-red fluorescent dye designed as a unique green-excitable option for super-resolution STORM imaging (STochastic Optical Reconstruction Microscopy). The dye was developed to address the lack of suitable dyes for multicolor STORM beyond red excitation channels. CF®597R was created by using a breakthrough chemical strategy for rhodamine-based dyes, allowing a green-excitable option with excellent solubility and photoswitching properties ideal for STORM imaging.
CF®597R and CF®583R, another green-excited fluorescent dye developed using a similar chemical strategy, were both validated for multicolor STORM imaging through a collaboration with UC Berkeley professor and STORM expert Ke Xu, PhD (Figure 2). The findings were published in Angewandte Chemie (read the full press release). For multicolor STORM, we recommend pairing CF®597R with CF®647, CF®660C, CF®680, or CF®750.
CF®597R is available as single-labeled secondary antibodies, amine-reactive succinimidyl esters, Mix-n-Stain™ CF® Dye Antibody Labeling Kits, and Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits.
CF®597R Technical Summary
- Abs/Em maxima: 597/619 nm
- Extinction coefficient: 115,000
- Molecular weight: ~800
- Laser line: 561 to 568 nm
- Direct replacement for: Alexa Fluor® 594, DyLight® 594, ATTO 594
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CF is a registered trademark of Biotium, Inc. Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific; Cy Dye is a registered trademark of Cytiva.