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Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits

Label your IgM antibody with one of our superior CF® Dyes in 15-30 minutes without a purification step.

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Catalog #
25 ug labeling
100 ug labeling
Clear selection
(Please select quantity above )

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Product Description

Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits allow you to label 25 ug or 100 ug of your IgM antibody in 15-30 minutes, with minimal hands-on time and no purification.


  • Label 25 ug or 100 ug IgM antibody
  • Choice of 8 CF® Dye colors and FITC
  • Less than 30 seconds of hands-on time
  • 15-30 minute total reaction time
  • No purification, 100% recovery

Kit components

  • Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
  • 10X Mix-n-Stain™ Reaction Buffer
  • Lyophilized Reactive CF® Dye
  • Mix-n-Stain™ IgM Storage Buffer

Finally! Simple & Efficient Labeling of IgM Antibodies

IgM antibodies are difficult to label with fluorescent dyes because the conditions typically used for labeling IgG generally do not result in bright conjugates with good antibody reactivity for IgM. Biotium has resolved this issue by optimizing the reaction conditions for labeling IgM antibodies with Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits.

Simply mix your purified IgM antibody with the reaction buffer and pre-measured dye provided, followed by a brief incubation. Any free dye or label in solution is no longer reactive at the end of the labeling, so the conjugate is ready for staining without further purification. The resulting conjugate is produced with optimal degree of labeling (DOL) for cell staining. Mix-n-Stain™ labeling is covalent, therefore labeled antibodies can be used for multiplex staining without the transfer of dye between proteins.

Superior CF® Dyes

These kits feature a choice of 8 of Biotium’s next-generation CF® Dyes, which were designed for advantages in brightness and photostability compared to other commercially available fluorescent dyes. Learn more about CF® Dyes.

Choose the Right Labeling Kit for Your Antibody

Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits are very simple to use. But before you begin, you must check that your antibody meets the compatibility requirements for labeling, and choose the right labeling protocol. Download the Product Protocol to help you determine if your IgM antibody is compatible with the labeling protocol, or if it requires further purification.

See the product table below for a quick reference of the maximum excitation and emission of each CF® Dye, or see our CF® Dye Brochure for more detailed dye information.

Other Labeling Kits

Check out our main line of Mix-n-Stain™ CF® Dye Antibody Labeling Kits and Mix-n-Stain™ Maxi Antibody Labeling Kits designed for rapid and efficient labeling of IgG antibodies. We also offer Mix-n-Stain™ Nanobody Labeling Kits for labeling single-domain Nanobodies®. Mix-n-Stain™ kits also are available for labeling antibodies with biotin, DNP, or DIG. See our full selection of Mix-n-Stain™ Antibody Labeling Kits.

Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits

ConjugationEx/EmSizeCatalog No.Dye Features
CF®405L413/547 nm25 ug92558CF®405L Features
100 ug92559
CF®405M416/452 nm25 ug92560CF®405M Features
100 ug92561
CF®488A490/516 nm25 ug92562CF®488A Features
100 ug92563
CF®555554/568 nm25 ug92564CF®555 Features
100 ug92565
CF®568562/583 nm25 ug92566CF®568 Features
100 ug92567
CF®594593/615 nm25 ug92568CF®594 Features
100 ug92569
CF®640R642/663 nm25 ug92570CF®640R Features
100 ug92571
CF®647652/668 nm25 ug92572CF®647 Features
100 ug92573
FITC498/517 nm25 ug92574
100 ug92575
Note: Before selecting a kit, please download the Product Protocol to see Kit Compatibility and Protocol Selection guidelines.

Antibody & Protein Labeling Kits

Mix-n-Stain™ CF® Dye
Antibody Labeling Kits
CF® Dyes≤5-20 ug IgG
20-50 ug IgG
50-100 ug IgG
~ 15 min.• Rapid, simple labeling

• No purification

• Tolerates BSA & other additives

• Dyes for super-resolution, spectral flow, & NIR detection
Mix-n-Stain™ FITC
Antibody Labeling Kits
Mix-n-Stain™ Cyanine Dye
Antibody Labeling Kits
Cyanine 555
Cyanine 647
Mix-n-Stain™ Biotin
Antibody Labeling Kits
Mix-n-Stain™ Digoxigenin
Antibody Labeling Kits
Digoxygenin (DIG)
Mix-n-Stain™ DNP
Antibody Labeling Kits
Mix-n-Stain™ Maxi
Antibody Labeling Kits
CF® Dyes
Cyanine Dyes
1 mg IgG~ 30 min.
Mix-n-Stain™ CF® Dye IgM Antibody Labeling KitsCF® Dyes
25 ug IgM
100 ug IgM
15-30 min.• Rapid, simple labeling

• No purification
Mix-n-Stain™ STORM CF® Dye Antibody Labeling KitsCF® Dyes50 ug IgG~ 30 min.• Optimized to provide low 1-2.5 DOL

• No purification
Nanobody Labeling Kits
CF® Dyes5-20 ug Nanobody®
20-50 ug Nanobody®
~ 30 min.• Optimized for Nanobodies®

• Tolerates BSA & other additives
Small Ligand Labeling Kits
CF® Dyes0.1 umol
small ligand
~ 30 min.• Label SNAP®, CLIP™, HaloTag® & other small ligands

• Dye options for surface or intracellular targets
VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo ImagingNear-IR CF® Dyes3 x 1 mg IgG~ 2 hours• Dyes, buffers, & spin vials for labeling + purification

• Sterile syringes, filters and storage vials for in vivo use
CF® Dye & Biotin SE
Protein Labeling Kits
CF® Dyes
3 x 1 mg
~ 2 hours• Dyes, buffers, & spin vials for labeling + purification
Nanobody is a registered trademark of Ablynx, a Sanofi company.


Download a list of curated CF® Dye references.


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