Mix-n-Stain™ small ligand labeling kits are designed for rapid labeling of low molecular weight (MW approx. 150 -5000) and relatively high affinity biological ligands (or substrates) without a purification step. The ligand to be labeled must contain an aliphatic amine group that is biologically inert. The amino group will form a covalent linkage with the provided reactive CF dyes. For example, suitable ligands or substrates include SNAP-tag®, CLIP-tag™ and HaloTag® ligand derivatives that have an aliphatic amine. For more information, please download the Mix-n-Stain™ small ligand labeling kit flyer and the product information sheet for this product.
This kit is for labeling the small ligand with red fluorescent CF™555 for intracellular staining of live cells.
Mix-n-Stain™ small ligand labeling kits for cell surface labeling are available in your choice of 10 CF™ dye colors for labeling ligands for cell surface targets, and three CF™ dye colors for ligands that bind intracellular targets. Also see our Mix-n-Stain™ Antibody Labeling Kits and other Reactive Dyes and Biotin Reagents.
|Dye||Catalog no.||Spectrally similar dyes||Ex (nm)||Em (nm)||Staining|
|CF405M||92362||Pacific Blue, BD Horizon V450||408||452||Surface|
|CF488A||92350||FITC, Alexa Fluor® 488||490||515||Surface|
|CF568||92351||Rhodamine Red, Alexa Fluor® 568||562||583||Surface|
|CF594||92352||Texas Red®, Alexa Fluor® 594||593||614||Surface|
|CF633||92353||Cy®5, Alexa Fluor® 633||630||650||Surface|
|CF640R||92354||Cy®5, Alexa Fluor® 647||642||662||Surface|
|CF647||92359||Cy®5, Alexa Fluor® 647||650||665||Surface|
|CF660C||92360||Alexa Fluor® 660||667||685||Surface|
|CF680||92361||Cy®5.5, Alexa Fluor® 660, IRDye®680LT||681||698||Surface|
|CF680R||92355||Cy®5.5, Alexa Fluor® 660, IRDye®680LT||680||701||Surface|
|CF408||92356||Pacific Blue, BD Horizon V450||408||450||Intracellular|
|CF500||92357||FITC, Alexa Fluor® 488||500||510||Intracellular|
|CF540||92358||Tetramethylrhodamine (TAMRA), TRITC, Alexa Fluor® 546, Cy®3||540||570||Intracellular|
|CF555||92364||Alexa Fluor® 555, Cy®3, TRITC||555||585||Intracellular|
|CF650||92363||Cy®5, Alexa Fluor® 647||650||670||Intracellular|
Mix-n-Stain and CF dyes are covered by US and international patents, and are trademarks of Biotium, Inc. SNAP-tag and CLIP-tag are trademarks or registered trademarks of New England BioLabs; HaloTag is a registered trademark of Promega Corporation; Pacific Blue, Texas Red, and Alexa Fluor are trademarks or registered trademarks of Invitrogen Corp; BD Horizon is a trademark of BD Biosciences; Cy Dye is a registered trademark of GE Healthcare; IRDye is a registered trademark of LI-COR Biosciences.