ANTIBODY & PROTEIN LABELING KITS

Mix-n-Stain™ antibody labeling kits dramatically simplify the process of antibody labeling. The resulting conjugates perform comparably to pre-labeled antibodies from leading suppliers. Because the labeling is covalent, the conjugates are stable for long-term storage, and ideal for multi-color imaging and multiplex flow cytometry.

Each Mix-n-Stain™ kit comes with everything you need to perform the conjugation. Without a separation step, you will have an optimally labeled antibody conjugate ready to be used in any immunofluorescence staining experiment.

One breakthrough feature of Mix-n-Stain™ kits is that antibody labeling can be conducted in the presence of many common buffer components such as BSA, gelatin, azide and low concentrations of Tris. As a result, the kits are compatible with most commercial antibody formulations without an antibody purification step prior to the conjugation process.

See below for our selection of Mix-n-Stain™ kits with a variety of label options.

With Mix-n-Stain™ CF® Dye Antibody Labeling Kits, you can covalently label as little as ≤5 ug up to 100 ug of an antibody in just 15 minutes, with minimal hands-on time and 100% recovery of antibody. With slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid, for unrivaled convenience. See the Compatibility and Protocol Selection Flowchart below, or download the Product Protocol for a step-by-step worksheet to determine kit compatibility with your antibody.

These kits feature a choice of over 30 superior CF® Dyes, which have advantages in brightness and photostability compared to other commercially available fluorescent dyes. Due to the high water solubility of CF® Dyes, other proteins like BSA or gelatin in the labeling reaction have minimal effect on non-specific background, because non-IgG proteins are washed away during IF staining. We also offer Mix-n-Stain™ kits for biotin, FITC, DIG, and DNP labeling, as well as Mix-n-Stain™ Maxi Antibody Labeling Kits for 1 mg scale labeling reactions.

Compatible with antibody stabilizers and ascites

Combine direct & indirect IF for flexible multiplexing

Mix-n-Stain™ CF® Dye, Biotin, FITC & Hapten IgG Antibody Labeling Kits

Finally! Simple & Efficient Labeling of IgM Antibodies

IgM antibodies are difficult to label with fluorescent dyes because the conditions typically used for labeling IgG generally do not result in bright conjugates with good antibody reactivity for IgM. Biotium has resolved this issue by optimizing the reaction conditions for labeling IgM antibodies with Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits.

Features

• Label 25 ug or 100 ug of IgM antibody
• Choice of 8 CF® Dye colors and FITC
• 15-30 minute total reaction time
• No purification, 100% recovery

Mix-n-Stain™ Nanobody Labeling Kits are designed specifically for labeling single-domain Nanobodies® (also called camelid single variable or VHH domains) with one of Biotium’s bright and photostable CF® Dyes or biotin. The kits allow optimal labeling of between 5-50 ug of Nanobody® in just 30 minutes, with minimal hands-on time and no purification. Labeling tolerates common buffer additives, including BSA.

The kits come with a selection of our next-generation CF® Dyes or biotin. CF® Dyes have advantages in brightness and photostability compared to other commercially available fluorescent dyes (learn more about CF® Dyes).

Features

• Label 5-20 ug or 20-50 ug of Nanobody®
• Choice of 7 CF® Dye colors or biotin
• Simple 30 minute labeling, no purification
• Compatible with common stabilizers
• Dye options for super-resolution and 2-photon imaging (learn more)

Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits offer a choice of 8 CF® Dyes developed with superior brightness and unique photoswitching properties that are ideal for super-resolution imaging by STORM. In addition, these specialized antibody labeling kits are designed to offer a low degree of labeling (DOL, or dye molecules per antibody molecule) of 1-2.5, which is reported to be optimal for STORM (Bittel et al. 2015). Biotium has also developed novel green-excited STORM dyes CF®583R and CF®597R to expand the color palette for multi-color STORM. Learn more about the UC Berkeley and Biotium collaboration paper on CF®583R and CF®597R dyes for STORM.

Features

• Choice of 8 CF® Dye colors ideal for STORM
• CF®583R and CF®597R are novel green-excited STORM dyes
• Labeling optimized to provide low 1-2.5 DOL
• Less than 30 seconds of hands-on time
• 30 minutes total reaction time
• No purification, 100% recovery

Learn more about CF® Dyes for STORM or our other fluorescent reagents validated for super-resolution microscopy.

Mix-n-Stain™ CF® Dye small ligand labeling kits are designed for rapid labeling of small (MW ~ 150 – 5,000) and relatively high affinity biological ligands (or substrates). Labeling takes about 30 minutes, without a final purification step. The ligands to be labeled must contain an aliphatic amine group that is not required for biological activity of the ligand. The amine group will form a covalent linkage with the reactive CF® Dye provided in the kit. For example, suitable ligands or substrates include SNAP-tag®, CLIP-tag™ and HaloTag® ligands with an aliphatic amine. Many other small ligands are also possible candidates if they meet the criteria described above.

Simply mix your small molecule ligand with the reaction buffer and the optimally formulated dye provided, and incubate for 30 minutes, followed by a brief 5 minute quenching step. No reactive Mix-n-Stain™ dye is available at the end of labeling; therefore the conjugate is ready for cell staining and imaging without further purification. Mix-n-Stain™ labeled ligands perform comparably to commercially-available pre-labeled ligands (Figure 1), at a fraction of the cost.

For live cell imaging, the Mix-n-Stain™ small ligand kits are ideal to generate fluorescent chemical tags that provide smaller, brighter, and more versatile alternatives to fluorescent proteins. Labeling of various chemical tags with Mix-n-Stain™ kits yielded highly efficient and specific labeling of the target proteins both on surface and inside of living cells. The Mix-n-Stain™ small ligand kits have also been proven compatible with fixed cell imaging and gel-based assays.

Features:

• Choice of 10 CF® Dye colors for extracellular staining
• Choice of 5 CF® Dye colors for intracellular staining
• For labeling molecules with a MW of 150-5000, containing an inactive aliphatic amine
• Suitable ligands or substrates include SNAP-tag®, CLIP-tag™ and HaloTag® ligands
• 30 minutes total reaction time, no purification necessary

CF® DYE SE
PROTEIN LABELING KITS

CF® Dye SE Protein Labeling Kits allow you to label your antibody of interest with one of our many bright and photostable CF® Dyes. The difference is that while the Mix-n-Stain™ kits come optimized for consistent labeling of small amounts of antibody, the SE Protein Labeling Kits are designed for 3 labelings of up to 1 mg protein with removal of free dye after labeling. The SE Protein Labeling Kits allow the user to optimize the amount of dye and antibody and determine the degree of protein labeling, for optimization of labeling and antibody performance.

Features:

• Choice of 19 CF® Dye colors or biotin
• Reagents for 3 labeling reactions with purification
• Each reaction can label up to 1 mg of antibody or protein
• Includes instructions for determining degree of labeling