FLOW CYTOMETRY REAGENTS

Our industry-leading CF® Dyes boast superior brightness over alternative fluorophores with exceptional signal-to-noise. In addition, CF® Dyes offer the widest selection of spectrally unique dyes to accommodate highly complex panels.

Primary Antibodies

• Growing collection of more than 1000 monoclonal antibodies: View Flyer
• Choose from 6 bright CF® Dyes or biotin
• Isotype control antibodies available conjugated to the same dyes and labels
• Available BSA-free, ready to use for Mix-n-Stain™ labeling
• Affordable 100 uL sizes available

Browse primary antibodies, or search with Antibody Finder

Primary Antibodies – sizes and formats:

Streptavidin & Anti-Biotin Secondary Antibodies

Streptavidin and anti-biotin antibodies available in 21 bright CF® Dyes, R-PE, APC, and PerCP

The Best Fixable Dead Cell Stains for Flow Cytometry:

• Wide selection of dye colors for easy panel design
• Exceptionally bright dyes for excellent live/dead separation
• No loss of brightness after fixation
• For flow cytometry or fluorescence microscopy
• Dyes designed to fit gaps in spectral flow panels
• The most options for the 633 nm and 808 nm lasers

Spectral flow cytometry is a rapidly growing technology with significantly enhanced multiplexing capabilities over conventional flow cytometry. Where conventional flow cytometer instruments can detect panels with more than a dozen fluorophores, spectral flow cytometers can accommodate panels with upwards of 40 dyes. Biotium offers spectrally unique Live-or-Dye™ stains designed specifically to take advantage of enhanced multiplexing by spectral flow cytometry.

Live-or-Dye™ Stains for Spectral Flow

New: Live-or-Dye™ Sampler Kits

Live-or-Dye™ stains are available in two sampler kits, each containing five different dye colors, for excitation from all of the popular flow cytometry laser lines (see Live-or-Dye™ Sampler Kits table below). The Live-or-Dye™ Sampler Kit, Standard (32016) was designed for use on the most common flow cytometer laser and filter configurations, with dyes excitable by UV, Violet, Blue, Yellow-Green, and Red lasers. The Live-or-Dye™ Sampler Kit, Spectral (32017) was designed for use in spectral scanning flow cytometry. It contains dyes excitable by UV, Violet, Blue, and Red lasers, all of which have been validated on the Cytek® Aurora spectral cytometer, and chosen for their ability to fill the gaps in many spectral flow panels.

Click the button on the right to view our our full selection of nuclear specific stains suitable for flow cytometry or microscopy.

RedDot™1 Far-Red Nuclear Stain

RedDot™1 is a novel far-red nuclear stain developed at Biotium. RedDot™1
is a live cell stain similar to DRAQ®5 that can be used for cell cycle
distribution analysis (Fig. 7). In addition, RedDot™1 cell cycle analysis does
not require an RNase step unlike the classic nuclear stain Propidium Iodide
(PI). RedDot™1 can also be used as a far-red nuclear counterstain for live
cells in microscopy.

• Far-red cell membrane-permeant nuclear dye similar to Draq5™.
• Can be used for DNA content analysis by flow cytometry like Vybrant™ DyeCycle™ Ruby
• Highly thermostable and photostable, for convenient handling

NucSpot® 470 Nuclear Stain

NucSpot® 470 is a cell membrane-impermeant green fluorescent DNA stain for nuclear counterstaining of fixed cells, or selective staining of dead cells.

• Nuclear-specific green counterstain for fixed cells
• Selective detection of dead cells by flow cytometry in the FITC channel
• Perform cell cycle profiling by flow cytometry in fixed/permeabilized cells (Fig. 8)
• No wash required

NucSpot® Far-Red Nuclear Stain

NucSpot® Far-Red is an improved alternative to the popular flow cytometry dead cell dye 7-AAD. It has red-shifted fluorescence emission compared to 7-AAD, for less bleed-through fluorescence in the PE-Texas Red® channel.

• Improved alternative to 7-AAD with less bleed-through fluorescence in the PE-Texas Red® channel
• Selective detection of dead cells by flow cytometry in the PE-Cy®5 or APC channel
• Perform cell cycle profiling by flow cytometry in fixed/permeabilized cells (Fig. 9)
• Substitute for 7-AAD in any standard protocol

NucView® Advantages

• Simple, homogeneous assay for endpoint assay or real-time detection in live cells
• Non-toxic, allowing for multi-day experiments
• Detect caspase-3 activity and visualize apoptotic nuclear morphology
• For flow cytometry, fluorescence microscopy, or live cell imaging systems
• Available as stand-alone substrates or in kits with other apoptosis or necrosis probes
• Available with blue, green, or red fluorescence
• NucView® 488 validated in more than 100 cell types and 200 publications – See the list

Mitochondrial Membrane Potential

Loss of mitochondrial membrane potential is a hallmark for apoptosis. Biotium offers MitoView™ 633 dye for membrane potential-sensitive staining of mitochondria by microscopy or flow cytometry. For simultaneous detection of mitochondrial membrane potential and caspase-3 activity, see the NucView®488 and MitoView™633 Apoptosis Assay (Fig. 13).

We also offer MitoView™ 405, which changes localization upon mitochondrial depolarization, and MitoView™ Green, a membrane-potential independent mitochondrial dye that can be used to image mitochondria following mitochondrial depolarization, or after fixation.

In healthy cells, JC-1 dye aggregates in mitochondria as a function of membrane potential, resulting in red fluorescence with brightness proportional to the membrane potential. Conversely, in apoptotic and necrotic cells with diminished mitochondrial membrane potential, JC-1 exists in a green fluorescent monomeric form in the cytosol, allowing of cell viability to be assessed by measuring the ratio of red to green fluorescence by flow cytometry or fluorescence microplate reader. Rhodamine 123 is a green fluorescent mitochondrial membrane potential dye. Red fluorescent TMRM and TMRE are the preferred dyes for quantitative membrane potential measurements.

Apoptosis/Necrosis Kits & Annexin V

Annexin V is a 36 kDa protein that has high affinity for phosphatidylserine (PS). During apoptosis, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be stained by fluorescent conjugates of Annexin V. Annexin V conjugates are available with a wide selection of CF® Dyes and other labels, including Near-IR CF® Dye Annexin V conjugates.

We offer many different staining kits with various combinations of dyes, including: NucView® caspase-3 substrate, our bright and photostable CF®488A Annexin V, a variety of dead cell nucleic acid stains, including propidium iodide (PI), 7-AAD, Ethidium Homodimer III (EthDIII), and RedDot™2. See the table below for more details on the various combination kits.

We offer dead cell nucleic acid stains separately as well, including far-red fluorescent RedDot™2 far-red dye and green fluorescent NucSpot® 470 stain.

We offer the following accessory products for your flow cytometry experiments:

• Flow Cytometry Fixation/Permeabilization Kit
• Fixation Buffer
• Permeabilization Buffer
• Permeabilization and Blocking Buffer (5X)
• Mini Cell Scrapers

View all of our flow cytometry accessory products.