Biotium Publications & Product Reference Lists
Reference Lists
Biotium Publications
Characterization of EvaGreen Dye and the implication of its physicochemical properties for qPCR applications
Mao, et al. BMC Biotechnology 7, 76 (2007)
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EvaGreen® Dye, 2000X in DMSO
EvaGreen® Dye, 20X in Water
Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells
Nocker, et al. Journal of Microbiological Methods 67 (2006)
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PMAxx™ Dye, 20 mM in H2O
DEVD-NucView488: a novel class of enzyme substrates for real-time detection of caspase-3 activity in live cells
Cen, et al. The FASEB Journal 22, 7 (2008)
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NucView® Caspase-3 Enzyme Substrates
NucView® 488 Caspase-3 Assay Kit for Live Cells
Tips for Avoiding Artifacts from UV Photoconversion of DAPI
Roberts, L. Microscopy Today, 27, 5 (2019)
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RedDot™2 Far-Red Nuclear Stain, 200X in DMSO
NucSpot® Live Cell Nuclear Stains
Small molecular fluorescence dyes for immuno cell analysis
Jiang, J., Li, X., Mao, F., Wu, X., & Chen, Y. Analytical Biochemistry, 114063 (2020)
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Mix-n-Stain™ CF® Dye Antibody Labeling Kits
Highlighted Citations
MitoView™ 633 offers advantages over TMRM as a probe for mitochondrial membrane potential
Ernst P, Kim S, Yang Z, Liu XM and Zhou L (2023) Characterization of the far-red fluorescent probe MitoView™ 633 for dynamic mitochondrial membrane potential measurement. Front. Physiol. 14:1257739. doi: 10.3389/fphys.2023.1257739
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MitoView™ Mitochondrial Dyes
ExoBrite™ CTB EV Stain assists in evaluating engineered EVs for targeted drug delivery against triple-negative breast cancer
Bhullar AS, Jin K, Shi H, Jones A, Hironaka D, Xiong G, Xu R, Guo P, Binzel DW, Shu D. Engineered extracellular vesicles for combinatorial TNBC therapy: SR-SIM-guided design achieves substantial drug dosage reduction. Mol Ther. 2024 Oct 5:S1525-0016(24)00658-0. doi: 10.1016/j.ymthe.2024.09.034. Epub ahead of print. PMID: 39369270.
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ExoBrite™ CTB EV Staining Kits
Tardigrade-derived protein shown to improve adipose stem cell survival against various stressors
Rolsma, J.L., Darch, W., Higgins, N.C. et al. The tardigrade-derived mitochondrial abundant heat soluble protein improves adipose-derived stem cell survival against representative stressors. Sci Rep 14, 11834 (2024). https://doi.org/10.1038/s41598-024-62693-w
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MitoView™ Fix 640
Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells
Arl2 GTPase associates with the centrosomal protein Cdk5rap2 to regulate cortical development via microtubule organization
Ma D, Lin K-Y, Suresh D, Lin J, Gujar MR, Aung HY, et al. (2024) Arl2 GTPase associates with the centrosomal protein Cdk5rap2 to regulate cortical development via microtubule organization. PLoS Biol 22(8): e3002751. https://doi.org/10.1371/journal.pbio.3002751
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ViaFluor® Live Cell Microtubule Stains
The DNA-PAINT palette: a comprehensive performance analysis of fluorescent dyes
Steen, P.R., Unterauer, E.M., Masullo, L.A. et al. The DNA-PAINT palette: a comprehensive performance analysis of fluorescent dyes. Nat Methods 21, 1755–1762 (2024). https://doi.org/10.1038/s41592-024-02374-8
APOE4/4 is linked to damaging lipid droplets in Alzheimer’s disease microglia
Haney, M.S., Pálovics, R., Munson, C.N. et al. APOE4/4 is linked to damaging lipid droplets in Alzheimer’s disease microglia. Nature 628, 154–161 (2024). https://doi.org/10.1038/s41586-024-07185-7
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LipidSpot™ Lipid Droplet Stains
Computational Investigations into Two-Photon Fibril Imaging Using the DANIR-2c Probe
N. Arul Murugan, Robert Zaleśny. Computational Investigations into Two-Photon Fibril Imaging Using the DANIR-2c Probe. J. Phys. Chem. B, 127, 14, 3119–3125 (2023). https://doi.org/10.1021/acs.jpcb.2c07783
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DCDAPH (DANIR-2c)
Intraocular liver spheroids for non-invasive high-resolution in vivo monitoring of liver cell function
Lazzeri-Barcelo, F., Oliva-Vilarnau, N., Baniol, M. et al. Intraocular liver spheroids for non-invasive high-resolution in vivo monitoring of liver cell function. Nat Commun 15, 767 (2024). https://doi.org/10.1038/s41467-024-45122-4
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Live-or-Dye NucFix™ Red Staining Kit
Anti-cancer target hRpn13 regulates proteome and transcriptome through epigenetic factors
Osei-Amponsa et al. (2023) Molecular Cell 84, 522–537 (2024) Published by Elsevier Inc., 2023, https://doi.org/10.1016/j.molcel.2023.11.035
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Mix-n-Stain™ CF® Dye Antibody Labeling Kits
Drop-n-Stain EverBrite™ Mounting Medium
A novel type of large EVs containing lipid droplets and mitochondria is released during melanoma cell division
Karbanová, J., Deniz, I. A., Wilsch-Bräuninger, M., de Sousa Couto, R. A., Fargeas, C. A., Santos, M. F., ... & Corbeil, D. (2024). Extracellular lipidosomes containing lipid droplets and mitochondria are released during melanoma cell division. Cell Communication and Signaling, 22(1), 57.
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LipidSpot™ Lipid Droplet Stains
Wheat Germ Agglutinin (WGA) Conjugates
MitoView™ Fix 640
Distinct neuroinflammatory signatures exist across genetic and sporadic amyotrophic lateral sclerosis cohorts
Rifai, O.M., O’Shaughnessy, J., et al (2023) Distinct neuroinflammatory signatures exist across genetic and sporadic amyotrophic lateral sclerosis cohorts (2023). Brain, Volume 146, Issue 12, 2023, 5124–5138, DOI: 10.1093/brain/awad243
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CELLDATA RNAstorm™ 2.0 FFPE RNA Extraction Kit
Gene regulation in progenitor cells is mediated through NUP98 and RAE1 chromatin binding in a HDAC dependent manner
Neely, A.E., Blumensaadt, L.A., Ho, P.J. et al. (2023) NUP98 and RAE1 sustain progenitor function through HDAC-dependent chromatin targeting to escape from nucleolar localization. Commun Biol 6, 664. https://doi.org/10.1038/s42003-023-05043-2
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Aquaphile™ JC-1
MitoView™ Mitochondrial Dyes
Post-Printing Modifications in 3D Bioprinting using Clickable Bioinks
Tournier, P., Saint‐Pé, G., Lagneau, N., Loll, F., Halgand, B., Tessier, A., ... & Delplace, V. (2023). Clickable Dynamic Bioinks Enable Post‐Printing Modifications of Construct Composition and Mechanical Properties Controlled over Time and Space. Advanced Science, 10(30), 2300055. https://doi.org/10.1002/advs.202300055
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CF® Dye Hydrazide
Advancing digital PCR-HRM assays for better pathogen identification
Lee, P.W., Chen, L., Hseih, K. et al (2023) Harnessing Variabilities in Digital Melt Curves for Accurate Identification of Bacteria (2023). Analytical Chemistry 2023 95 (42), 15522-15530. DOI: 10.1021/acs.analchem.3c01654
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EvaGreen® Dye, 20X in Water
EvaGreen® Plus Dye, 20X in Water
Biotium CF® Dyes aid in the discovery of MSC‑associated migrasomes as a novel source of chemoattractant for cells of hematopoietic origin
Deniz, I. A., Karbanová, J., Wobus, M., Bornhäuser, M., Wimberger, P., Kuhlmann, J. D., & Corbeil, D. (2023). Mesenchymal stromal cell-associated migrasomes: a new source of chemoattractant for cells of hematopoietic origin. Cell Communication and Signaling, 21(1), 1-17. https://doi.org/10.1186/s12964-022-01028-6
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Wheat Germ Agglutinin (WGA) Conjugates
ExoBrite™ WGA EV Staining Kits
Rapid Detection of Predominant SARS-CoV-2 Variants Using EvaGreen® Dye in Multiplex High-Resolution Melting Analysis
Sun, L., Wang, L., Zhang, C. et al (2023). Rapid detection of predominant SARS-COV-2 variants using multiplex high-resolution melting analysis. Microbiology Spectrum (2023). https://journals.asm.org/doi/10.1128/spectrum.00055-23
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EvaGreen® Dye, 20X in Water
EvaGreen® Plus Dye, 20X in Water
The molecular architecture of the photoreceptor active zone resolved with CF®660C Dye in MINFLUX
Grabner, C. P., Jansen, I., Neef, J., Weihs, T., Schmidt, R., Riedel, D., ... & Moser, T. (2022). Resolving the molecular architecture of the photoreceptor active zone with 3D-MINFLUX. Science Advances, 8(28), eabl7560. DOI: 10.1126/sciadv.abl7560
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Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits
Mix-n-Stain™ labeling used in epitope mapping of novel NANOBODIES® for the Alzheimer’s-related receptor SORLA
Monti, G., Vincke, C., Lunding, M., Jensen, A. M. G., Madsen, P., Muyldermans, S., ... & Andersen, O. M. (2023). Epitope mapping of nanobodies binding the Alzheimer’s disease receptor SORLA. Journal of Biotechnology. https://doi.org/10.1016/j.jbiotec.2023.08.005
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Mix-n-Stain™ Nanobody Labeling Kits
Enhanced axial resolution in three-dimensional structured illumination microscopy using CellBrite® Fix Membrane Dyes
Li, X., Wu, Y., Su, Y., Rey-Suarez, I., Matthaeus, C., Updegrove, T. B., ... & Shroff, H. (2023). Three-dimensional structured illumination microscopy with enhanced axial resolution. Nature Biotechnology, 1-13. https://doi.org/10.1038/s41587-022-01651-1
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CellBrite® Fix Membrane Stains
Development of novel PE and APC near-infrared tandem fluorochromes for use in spectral flow cytometry
Seong, Y., Nguyen, D. X., Wu, Y., Thakur, A., Harding, F., & Nguyen, T. A. (2022). Novel PE and APC tandems: Additional near‐infrared fluorochromes for use in spectral flow cytometry. Cytometry Part A, 101(10), 835-845. https://doi.org/10.1002/cyto.a.24537
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CF® Dye & Biotin SE Protein Labeling Kits
Mix-n-Stain™ CF® Dye Antibody Labeling Kits
High-resolution characterization of centriole morphology, dynamics, and abnormalities using correlative STORM and electron microscopy
Bowler, M., Kong, D., Sun, S., Nanjundappa, R., Evans, L., Farmer, V., ... & Loncarek, J. (2019). High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy. Nature communications, 10(1), 993.
Vásquez-Limeta, A., Lukasik, K., Kong, D., Sullenberger, C., Luvsanjav, D., Sahabandu, N., ... & Loncarek, J. (2022). CPAP insufficiency leads to incomplete centrioles that duplicate but fragment. Journal of Cell Biology, 221(5), e202108018.
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Goat Anti-Mouse IgG (H+L), F(ab')2 Fragment
Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment
Exosome-delivered miRNA promotes breast cancer progression in African Americans by activating cancer-associated fat cells
Zhao, D., Wu, K., Sharma, S., Xing, F., Wu, S. Y., Tyagi, A., ... & Watabe, K. (2022). Exosomal miR-1304-3p promotes breast cancer progression in African Americans by activating cancer-associated adipocytes. Nature Communications, 13(1), 7734. https://www.nature.com/articles/s41467-022-35305-2
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LipidSpot™ Lipid Droplet Stains
Usage of the NanoString® platform to evaluate the effect of RNA integrity on gene expression profiling of FFPE samples
Manjunath, H. S., Al Khulaifi, M., Sidahmed, H., Ammar, A., Vadakekolathu, J., Rutella, S., et al. Gene Expression Profiling of FFPE Samples: A Titration Test. Technology in Cancer Research & Treatment, 21, 15330338221129710 (2022). https://doi.org/10.1177/15330338221129
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CELLDATA RNAstorm™ FFPE RNA Extraction Kit
Biotium dyes and antibodies help to demonstrate a novel anchoring strategy for expansion microscopy
Klimas, A., Gallagher, B. R., Wijesekara, P., et al. Magnify is a universal molecular anchoring strategy for expansion microscopy. Nat Biotechnol 1-12 (2023). https://doi.org/10.1038/s41587-022-01546-1
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Wheat Germ Agglutinin (WGA) Conjugates
CF® Dye SE/TFP Ester
Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed (Min X Rat)
Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed
Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed
GelRed® analysis of contaminating endogenous cfDNA from FBS
Werner, B., Warton, K., & Ford, C. E. Endogenous cell-free DNA in fetal bovine serum introduces artifacts to in vitro cell-free DNA models. BioTechniques, 73(5), 219-226 (2022). https://doi.org/10.2144/btn-2022-0040
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GelRed® Nucleic Acid Gel Stain
Biotium dyes help demonstrate an acousto-optic tunable filter (AOTF)-based system for excitation spectral microscopy super-multiplexing
Chen, K., Li, W., & Xu, K. (2022). Super-multiplexing excitation spectral microscopy with multiple fluorescence bands. Biomedical Optics Express, 13(11), 6048-6060. https://doi.org/10.1364/BOE.473241
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LipidSpot™ Lipid Droplet Stains
Goat Anti-Mouse IgG1 (γ1)
Goat Anti-Rabbit IgG (H+L)
Goat Anti-Chicken IgY (H+L), Highly Cross-Adsorbed
Wheat Germ Agglutinin (WGA) Conjugates
Glucose oxidase-dependent deposition of fluorochromized tyramide (FT-GO) for sensitive multiplex immunofluorescence and in situ hybridization labeling
Yamauchi, K., Okamoto, S., Ishida, Y., et al. Fluorochromized tyramide-glucose oxidase as a multiplex fluorescent tyramide signal amplification system for histochemical analysis. Scientific Reports, 12(1), 1-12 (2022). https://doi.org/10.1038/s41598-022-19085-9
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CF® Dye Tyramide
Nuclei bypass intercellular obstacles by deforming around them with deep local invaginations mediated by lamin A/C
Katiyar, A., Zhang, J., Antani, J. D. et al. The nucleus bypasses obstacles by deforming like a drop with surface tension mediated by lamin A/C. Advanced Science, 9(23), 2201248 (2022). https://doi.org/10.1002/advs.202201248
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NucSpot® Live Cell Nuclear Stains
Fluoroamphiphile-based nanocarriers for intracellular drug delivery and organelle targeting
Liu, Y., Zhang, J., Tu, Y., & Zhu, L. Potential-independent intracellular drug delivery and mitochondrial targeting. ACS nano, 16(1), 1409-1420 (2021). https://doi.org/10.1021/acsnano.1c09456
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MitoView™ Mitochondrial Dyes
An optimized universal hairpin primer system for RT-qPCR-based quantification of miRNA expression
He, F., Ni, N., Wang, H., et al. OUHP: an optimized universal hairpin primer system for cost-effective and high-throughput RT-qPCR-based quantification of microRNA (miRNA) expression. Nucleic acids research, 50(4), e22-e22 (2022).
https://academic.oup.com/nar/article/50/4/e22/6440322
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Forget-Me-Not™ EvaGreen® qPCR Master Mix (2-Color Tracking)
Single particle counting using fluorescent antibodies demonstrated as a promising diagnostic tool for neurodegenerative diseases
Blömeke, L., et al. Quantitative detection of α-Synuclein and Tau oligomers and other aggregates by digital single particle counting. npj Parkinson's Disease 8.1 1-13 (2022). https://doi.org/10.1038/s41531-022-00330-x
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Mix-n-Stain™ CF® Dye Antibody Labeling Kits
CF® Dye & Biotin SE Protein Labeling Kits
CF® Dye SE/TFP Ester
TrueBlack® Lipofuscin Autofluorescence Quencher is the preferred quencher for detection of mRNA in human neuronal populations
May‐Zhang, A. A., Benthal, J. T., & Southard‐Smith, E. M. (2022). Hybridization Chain Reaction for mRNA Localization in Single Cells from Mouse and Human Cryosections. Current Protocols, 2(5), e439.https://doi.org/10.1002/cpz1.439
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TrueBlack® Lipofuscin Autofluorescence Quencher
TrueBlack® Plus Lipofuscin Autofluorescence Quencher, 40X in DMSO
A new high-throughput and broadband photoacoustic microscopy technique validated with near-infrared CellBrite® Dyes
Takeshi H., Kazuyoshi T., Manami M., Shinpei O., Toshihiro K., Miya I. (2022). Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay. Photoacoustics, 26, 2213-5979. https://doi.org/10.1016/j.pacs.2022.100364
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CellBrite® Cytoplasmic Membrane Dyes
CellBrite® NIR Cytoplasmic Membrane Dyes
A chip-based assay for detection and avidity assessment of SARS-CoV-2 antibodies in serum and saliva
Liu, T., Hsiung, J., Zhao, S., Kost. et al. Quantification of antibody avidities and accurate detection of SARS-CoV-2 antibodies in serum and saliva on plasmonic substrates. Nature Biomedical Engineering 4:12, 4(12), 1188–1196 (2020). https://doi.org/10.1038/s41551-020-00642-4
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CF® Dye SE/TFP Ester
Mix-n-Stain™ CF® Dye Antibody Labeling Kits
CF® Dye & Biotin SE Protein Labeling Kits
Introduction of extracellular matrix inhibits glioma cell proliferation
Murdock, M. H., Hussey, G. S., Chang, J. T., Hill, R. C., Nascari, D. G., Rao, A. V., Hansen, K. C., Foley, L. M., Hitchens, T. K., Amankulor, N. M., & Badylak, S. F. (2022). A liquid fraction of extracellular matrix inhibits glioma cell viability in vitro and in vivo. Oncotarget, 13, 426–438. https://doi.org/10.18632/oncotarget.28203
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NucView® 488 Caspase-3 Assay Kit for Live Cells
NucView® Caspase-3 Enzyme Substrates
GelRed® used to assess efficiency of metallohelices as gene delivery vectors
Malina, J., Kostrhunova, H., Novohradsky, V. et al. (2022) Metallohelix vectors for efficient gene delivery via cationic DNA nanoparticles, Nucleic Acids Research. https://doi.org/10.1093/nar/gkab1277
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GelRed® Nucleic Acid Gel Stain
GelRed® Prestain Plus 6X DNA Loading Dye
GelRed® Agarose LE
Multiview confocal microscopy protocol for improved imaging in thick samples
Wu, Y., Han, X., Su, Y. et al. (2021) Multiview confocal super-resolution microscopy. Nature. https://doi.org/10.1038/s41586-021-04110-0
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NucSpot® Live Cell Nuclear Stains
UC Berkeley and Biotium identify dye modifications for superior fluorescence photoswitching in STORM
Wang, B., Xiong, M., Susanto, J., Li, X., Leung, W.-Y., & Xu, K. (2021). Transforming Rhodamine Dyes for (d)STORM Super-Resolution Microscopy via 1,3-Disubstituted Imidazolium Substitution. Angewandte Chemie International Edition. https://doi.org/10.1002/ANIE.202113612
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CF® Dye SE/TFP Ester
Mix-n-Stain™ CF® Dye Antibody Labeling Kits
Phalloidin Conjugates
Endocytosis of cationic molecules requires Rab14, but not Rab5 and Rab7
Trofimenko, E., Homma, Y., Fukuda, M., & Widmann, C. (2021). The endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab7. Cell Reports, 37(5), 109945. https://doi.org/10.1016/j.celrep.2021.109945
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Mix-n-Stain™ CF® Dye Small Ligand Labeling Kits
TrueBlack® added to resin embedding medium improves signal-to-noise in brain tissue
Ren, M., Tian, J., Sun, Q., Chen, S., Luo, T., Jia, X., Jiang, T., Luo, Q., Gong, H., & Li, X., (2021). Plastic embedding for precise imaging of large-scale biological tissues labeled with multiple fluorescent dyes and proteins. Biomed. Opt. Express 12, 6730-6745. https://doi.org/10.1364/BOE.435120
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TrueBlack® Lipofuscin Autofluorescence Quencher
TrueBlack® Plus Lipofuscin Autofluorescence Quencher, 40X in DMSO
Novel nanoformulated antiretroviral therapy for HIV treatment with improved drug targeting
Martinez-Skinner AL, Araínga MA, Puligujja P, Palandri DL, Baldridge HM, Edagwa BJ, et al. (2015) Cellular Responses and Tissue Depots for Nanoformulated Antiretroviral Therapy. PLOS One 10(12): e0145966. https://doi.org/10.1371/journal.pone.0145966
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CF® Dye SE/TFP Ester
Mix-n-Stain™ CF® Dye Antibody Labeling Kits
Viability PCR with PMA validated for determination of infectious SARS-CoV-2 viruses
Hong, W., Xiong, J., Nyaruaba, R., Li, J., Muturi, E., Liu, H., Yu, J., Yang, H., & Wei, H. (2021). Rapid determination of infectious SARS-CoV-2 in PCR-positive samples by SDS-PMA assisted RT-qPCR. The Science of the total environment, 797, 149085. Advance online publication. https://doi.org/10.1016/j.scitotenv.2021.149085
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PMA (Propidium Monoazide)
PMAxx™ Dye, 20 mM in H2O
Voltage dye Di-4-ANEPPS used to develop improved optical mapping of rat hearts
Ramlugun, G. S., Sands, G. B., Zhao, J., LeGrice, I. J., & Smaill, B. H. (2021). A novel system for mapping regional electrical properties and characterizing arrhythmia in isolated intact rat atria. American journal of physiology. Heart and circulatory physiology, 321(2), H412–H421. https://doi.org/10.1152/ajpheart.00185.2021
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Di-4-ANEPPS
Di-8-ANEPPS
DiBAC4(3)
RT-LAMP assays using in-house produced enzymes show promise for accurate and affordable SARS-CoV-2 testing
Alekseenko, A., Barrett, D., Pareja-Sanchez, Y. et al. Direct detection of SARS-CoV-2 using non-commercial RT-LAMP reagents on heat-inactivated samples. Sci Rep 11, 1820 (2021). https://doi.org/10.1038/s41598-020-80352-8
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EvaGreen® Dye, 20X in Water
EvaGreen® Plus Dye, 20X in Water
Forget-Me-Not™ EvaGreen® qPCR Master Mix (2-Color Tracking)
A sensitive EvaGreen-based PCR assay for measuring dNTP concentrations in cells and tissue
Purhonen, J., Banerjee, R., McDonald, A.E., Fellman, V., Kallijärvi, J., A sensitive assay for dNTPs based on long synthetic oligonucleotides, EvaGreen dye and inhibitor-resistant high-fidelity DNA polymerase. Nucleic Acids Research 48, e87 (2020). https://doi.org/10.1093/nar/gkaa516
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EvaGreen® Dye, 20X in Water
PAGE GelRed® Nucleic Acid Gel Stain
PICASSO: Ultra-multiplexed fluorescence imaging of biomolecules through single-round imaging and blind source unmixing
Seo, J., Sim, Y., Kim, J., Kim, H., Cho, I., Yoon, Y., & Chang, J.-B.(2022) PICASSO: Ultra-multiplexed fluorescence imaging of biomolecules through single-round imaging and blind source unmixing. Nat Commun 13, 2475. https://doi.org/10.1038/s41467-022-30168-z
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CF® Dye SE/TFP Ester
Mix-n-Stain™ CF® Dye Antibody Labeling Kits
Exosome profiling with CF® dye tetraspanin panel shows single tetraspanin immunocapture methods may limit cancer biomarker detection
Mizenko, R. R., Brostoff, T., Rojalin, T., Koster, H. J., Swindell, H. S., Leiserowitz, G. S., … Carney, R. P. (2021). Tetraspanin immunocapture phenotypes extracellular vesicles according to biofluid source but may limit identification of multiplexed cancer biomarkers. BioRxiv, 2021.03.02.433595. https://doi.org/10.1101/2021.03.02.433595
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CD9 Monoclonal Mouse Antibody (CD9/1619)
CD63 Monoclonal Mouse Antibody (MX-49.129.5)
CD81 / TAPA-1 Monoclonal Mouse Antibody (1.3.3.22)
Spectrally distinct CF® Dyes enable a 43 color high dimensional spectral flow panel designed to characterize human peripheral blood mononuclear cells
Sahir F, Mateo JM, Steinhoff M, Siveen KS. Development of a 43 color panel for the characterization of conventional and unconventional T-cell subsets, B cells, NK cells, monocytes, dendritic cells, and innate lymphoid cells using spectral flow cytometry. Cytometry. 2020;1–7. https://doi.org/10.1002/cyto.a.24288
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Mix-n-Stain™ CF® Dye Antibody Labeling Kits
Fast three-color single-molecule FRET using statistical inference
Yoo, J., Kim, JY., Louis, J.M. et al. Fast three-color single-molecule FRET using statistical inference. Nat Commun 11, 3336 (2020). https://doi.org/10.1038/s41467-020-17149-w
Kim, JY and Chung, HS, Disordered Proteins Follow Diverse Transition Paths as They Fold and Bind to a Partner. Science 368, 1253 (2020) https://doi.org/10.1126/science.aba3854
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CF® Dye Maleimides
Mix-n-Stain™ CF® Dye Antibody Labeling Kits
Measuring mitochondrial mass as an indicator of cellular health and functional autophagy
Zijian Tang, Kuo-Hui Su, Meng Xu, & Chengkai Dai (2020) HSF1 physically neutralizes amyloid oligomers to empower overgrowth and bestow neuroprotection. Science Advances https://doi.org/10.1126/sciadv.abc6871
Yang, G., Song, W., Xu, J. et al. (2020) Pik3c3 deficiency in myeloid cells imparts partial resistance to experimental autoimmune encephalomyelitis associated with reduced IL-1β production. Cell Mol Immunol. https://doi.org/10.1038/s41423-020-00589-1
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MitoView™ Mitochondrial Dyes
CF® Dyes light the way for high dimensional analysis by spectral flow cytometry
Jiang, J., Li, X., Mao, F., Wu, X., & Chen, Y. (2020). Small molecular fluorescence dyes for immuno cell analysis. Analytical Biochemistry, 114063. https://doi.org/10.1016/j.ab.2020.114063
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Mix-n-Stain™ CF® Dye Antibody Labeling Kits
Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity
L. Cantuti-Castelvetri et al. (2020), Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity Science https://doi.org/10.1126/science.abd2985
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Mix-n-Stain™ CF® Dye Antibody Labeling Kits
CF® Dye SE/TFP Ester
Fast and multiplexed super-resolution imaging with DNA-PAINT-ERS
Civitci, F., Shangguan, J., Zheng, T., Tao, K., Rames, M., Kenison, J., Zhang, Y., Wu, L., Phelps, C., Esener, S., Nan, X. (2020). In Vivo Single-Molecule Detection of Nanoparticles for Multiphoton Fluorescence Correlation Spectroscopy to Quantify Cerebral Blood Flow. Nature Communications. https://doi.org/10.1038/s41467-020-18181-6
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Mix-n-Stain™ CF® Dye Antibody Labeling Kits
Novel Method for Monitoring Cerebral Blood Flow with Subvessel Resolution In Vivo
Fu, X., Sompol, P., Brandon, J., Norris, C., Wilkop, T., Johnson, L., & Richards, C. (2020). In Vivo Single-Molecule Detection of Nanoparticles for Multiphoton Fluorescence Correlation Spectroscopy to Quantify Cerebral Blood Flow. Nano Letters. https://doi.org/10.1021/acs.nanolett.0c02280
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CF® Dye Dextran 3,500 MW, Anionic and Fixable
Sensitivity assessment of RT-qPCR and droplet digital PCR for SARS-CoV-2 detection
Falzone, L., Musso, N., Gattuso, G., Bongiorno, D., Palermo, C., Scalia, G., … Stefani, S. (2020). Sensitivity assessment of droplet digital PCR for SARS-CoV-2 detection. International Journal of Molecular Medicine, 46(3), 957–964. https://doi.org/10.3892/ijmm.2020.4673
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Sun, F., Ganguli, A., Nguyen, J., Brisbin, R., Shanmugam, K., Hirschberg, D. L., … Cunningham, B. T. (2020). Smartphone-Based Multiplex 30-minute Nucleic Acid Test of Live Virus from Nasal Swab Extract. Lab on a Chip. https://doi.org/10.1039/D0LC00304B
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Hayashida K, Orba Y, Sequeira PC, Sugimoto C, Hall WW, Eshita Y, Suzuki Y, Runtuwene L, Brasil P, Calvet G, Rodrigues CDS, Santos CCD, Mares-Guia MAM, Yamagishi J, Filippis AMB, Sawa H. Field diagnosis and genotyping of chikungunya virus using a dried reverse transcription loop-mediated isothermal amplification (LAMP) assay and MinION sequencing. PLoS Negl Trop Dis. 2019 Jun 3;13(6):e0007480. doi: 10.1371/journal.pntd.0007480.
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Pang, L., Ding, J., Ge, Y., Fan, J., & Fan, S. K. (2019). Single-Cell-Derived Tumor-Sphere Formation and Drug-Resistance Assay Using an Integrated Microfluidics. Analytical chemistry.
https://pubs.acs.org/doi/10.1021/acs.analchem.9b01084
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Compte M, Harwood SL, Muñoz IG, Navarro R, Zonca M, Perez-Chacon G, Erce-Llamazares A, Merino N, Tapia-Galisteo A, Cuesta AM, Mikkelsen K, Caleiras E, Nuñez-Prado N, Aznar MA, Lykkemark S, Martínez-Torrecuadrada J, Melero I, Blanco FJ, Bernardino de la Serna J, Zapata JM, Sanz L, Alvarez-Vallina L. A tumor-targeted trimeric 4-1BB-agonistic antibody induces potent anti-tumor immunity without systemic toxicity. Nat Commun. 2018 Nov 15;9(1):4809. doi:10.1038/s41467-018-07195-w.
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Farmer BC, Kluemper J, Johnson LA. Apolipoprotein E4 Alters Astrocyte Fatty Acid Metabolism and Lipid Droplet Formation. Cells. 2019 Feb 20;8(2). pii: E182. doi: 10.3390/cells8020182.
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Akram KM, Yates LL, Mongey R, Rothery S, Gaboriau DCA, Sanderson J, Hind M, Griffiths M, Dean CH. Live imaging of alveologenesis in precision-cut lung slices reveals dynamic epithelial cell behaviour. Nat Commun. 2019 Mar 12;10(1):1178.doi: 10.1038/s41467-019-09067-3.
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Checinska Sielaff, A.,Urbaniak, C.,Mohan, G. B. M.,Stepanov, V. G.,Tran, Q.,Wood, J. M.,Minich, J.,McDonald, D.,Mayer, T.,Knight, R., et al. (2019). Characterization of the total and viable bacterial and fungal communities associated with the International Space Station surfaces. Microbiome 7, 50.
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Vesicle-Cloaked Virus Clusters Are Optimal Units for Inter-organismal Viral Transmission. Cell Host Microbe. 2018 Aug 8;24(2):208-220.e8. doi:10.1016/j.chom.2018.07.006.
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Harper J, Adams KJ, Bossi G, Wright DE, Stacey AR, Bedke N, et al. (2018) An approved in vitro approach to preclinical safety and efficacy evaluation of engineered T cell receptor anti-CD3 bispecific (ImmTAC) molecules. PLoS ONE 13(10):e0205491.
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De P, Carlson JH et al. Triple Fluorescence staining to Evaluate Mechanism-based Apoptosis following Chemotherapeutic and Targeted Anti-cancer Drugs in Live Tumor Cells. Sci Rep. 2018;8(1):13192.doi: 10.1038/s41598-018-31575-3.
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Hauser, Meghan, Rui Yan, Wan Li, Nicole A. Repina, David V. Schaffer, and Ke Xu. The Spectrin-Actin-Based Periodic Cytoskeleton as a Conserved Nanoscale Scaffold and Ruler of the Neural Stem Cell Lineage. Cell Reports 24, no. 6 (2018): 1512-1522. https://doi.org/10.1016/j.celrep.2018.07.005
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Sapoń, K, Janas, T, Sikorski, AF, and Janas, T. (2018). Polysialic acid chains exhibit enhanced affinity for ordered regions of membranes. BBA-Biomembranes https://doi.org/10.1016/j.bbamem.2018.07.008
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Spangler B, Dovala D, Sawyer WS, Thompson KV, Six DA, Reck F, Feng BY. Molecular Probes for the Determination of Subcellular Compound Exposure Profiles in Gram-Negative Bacteria. ACS Infect Dis. 2018 Jun 15. doi:10.1021/acsinfecdis.8b00093.
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Smedowski A, Pietrucha-Dutczak M, Maniar T, Ajeleti M, Matuszek I, and Lewin-Kowalik J. Oxidative Medicine and Cellular Longevity. (2018), Article ID 2487473. doi.org/10.1155/2018/2487473
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Tsougeni K, Ellinas K, Koukouvinos G, Petrou PS, Tserepi A, Kakabakos SE, Gogolides E. Three-dimensional (3D) plasma micro-nanotextured slides for high performance biomolecule microarrays: Comparison with epoxy-silane coated glass slides. Colloids Surf B Biointerfaces. (2018), 165:270-277. doi:10.1016/j.colsurfb.2018.02.055.
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(1) Chen W, Yu H, et al. Mobile Platform for Multiplexed Detection and Differentiation of Disease-Specific Nucleic Acid Sequences, Using Microfluidic Loop-Mediated Isothermal Amplification and Smartphone Detection. Anal Chem. 2017 Nov 7;89(21):11219-11226. doi: 10.1021/acs.analchem.7b02478. (2) Ganguli A, Ornob A, et al. Hands-free smartphone-based diagnostics for simultaneous detection of Zika, Chikungunya, and Dengue at point-of-care. Biomed Microdevices. 2017 Aug 22;19(4):73. doi: 10.1007/s10544-017-0209-9.
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Hariri A, Lemaster J, Wang J, Jeevarathinam AS, Chao DL, Jokerst JV. The characterization of an economic and portable LED-based photoacoustic imaging system to facilitate molecular imaging. Photoacoustics. 2017 Nov 26;9:10-20. doi:10.1016/j.pacs.2017.11.001.
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Boothe T, Hilbert L, Heide M, Berninger L, Huttner WB, Zaburdaev V, Vastenhouw NL, Myers EW, Drechsel DN, Rink JC. (2017). A tunable refractive index matching medium for live imaging cells, tissues and model organisms. Elife. 14;6. DOI: 10.7554/eLife.27240
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Lim A, Rechtsteiner A, Saxton WM. (2017) Two kinesins drive anterograde neuropeptide transport. Mol Biol Cell. 28(24):3542-3553. https://doi: 10.1091/mbc.E16-12-0820
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Guisier F, Bohn P, Patout M, Piton N, Farah I, Vera P, et al. (2017) In- and ex-vivo molecular imaging of apoptosis to assess sensitivity of non-small cell lung cancer to EGFR inhibitors using probe-based confocal laser endomicroscopy. PLoS ONE 12(7): e0180576. https://doi.org/10.1371/journal.pone.0180576
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Danielle M. Jorgens, Jamie L. Inman, Michal Wojcik, Claire Robertson, Hildur Palsdottir, Wen-Ting Tsai, Haina Huang, Alexandre Bruni-Cardoso, Claudia S. López, Mina J. Bissell, Ke Xu, Manfred Auer. Deep nuclear invaginations are linked to cytoskeletal filaments - integrated bioimaging of epithelial cells in 3D culture. J Cell Sci 2017 130: 177-189; doi: 10.1242/jcs.190967
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Mayer T, et al.Microbial succession in an inflated lunar/Mars analog habitat during a 30-day human occupation. Microbiome. June 2016.
DOI: 10.1186/s40168-016-0167-0
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Escue, R. Kandasamy, K. and Parthasarathi, K. Thrombin Induces Inositol Trisphosphate-Mediated Spatially Extensive Responses in Lung Microvessels. Am J. Pathol 2017 doi: 10.1016/j.ajpath.2016.12.014
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Holton AB, Sinatra FL, Kreahling J, Conway AJ, Landis DA, Altiok S (2017) Microfluidic Biopsy Trapping Device for the Real-Time Monitoring of Tumor Microenvironment. PLoS ONE 12(1): e0169797. doi:10.1371/journal.pone.0169797
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T. Gilboa, C. Torfstein, M. Juhasz, A. Grunwald, Y. Ebenstein, E. Weinhold, and A. Meller. Single-Molecule DNA Methylation Quantification Using Electro-optical Sensing in Solid-State Nanopores. ACS Nano 2016, 10, 8861−8870. DOI: 10.1021/acsnano.6b04748
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Pulsipher A, Qin X, Thomas AJ, Prestwich GD, Oottamasathien S, Alt JA. Prevention of sinonasal inflammation by a synthetic glycosaminoglycan. Int Forum Allergy Rhinol. 2016. DOI: 10.1002/alr.21865
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Sensitive RT-LAMP detection of malaria infection using GelGreen® dye
Kemleu S, Guelig D, Eboumbou Moukoko C, Essangui E, Diesburg S, Mouliom A, et al.
A Field-Tailored Reverse Transcription Loop-Mediated Isothermal Assay for High Sensitivity Detection of Plasmodium falciparum Infections. 2016, PLOS ONE 11(11), Nov 8;11(11):e0165506. doi: 10.1371/journal.pone.0165506.
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GelGreen® Nucleic Acid Gel Stain
Evaluating the role of chitosan as an adjuvant for an intranasal HBV vaccine
Wang, Zhi-Biao, Pu Shan, Su-Zhen Li, Ya Zhou, Xia Deng, Ji-Lai Li, Yu Zhang, Jin-Shuang Gao, and Jing Xu. "The mechanism of action of acid-soluble chitosan as an adjuvant in the formulation of nasally administered vaccine against HBV." RSC Advances, 2016,6, 96785-96797. doi: 10.1039/C6RA14419E.
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CF® Dye SE/TFP Ester
Evaluation of dental plaque and halitosis in dogs by PMA and qPCR
Jeusette IC, Román AM, Torre C, Crusafont J, Sánchez N, Sánchez MC, Pérez-Salcedo L, Herrera D. "24-hour evaluation of dental plaque bacteria and halitosis after consumption of a single placebo or dental treat by dogs." Am J Vet Res, June 2016, Vol. 77, No. 6 , Pages 613-619. doi: 10.2460/ajvr.77.6.613
Single cell microarray for cell viability and comet assay in adherent cells
Lili Li, Weixing Wang, Mingyu Ding, Guoan Luo, and Qionglin Liang. Single-Cell-Arrayed Agarose Chip for in Situ Analysis of Cytotoxicity and Genotoxicity of DNA Cross-Linking Agents.Anal. Chem. DOI: 10.1021/acs.analchem.6b01008 (2016).
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GelRed® Nucleic Acid Gel Stain
Best dyes for quantification of low amounts of DNA
B. Bruijns, R. Tiggelaar, H. Gardeniers, Fluorescent cyanine dyes for the
quantification of low amounts of dsDNA, Analytical Biochemistry (2016), doi: 10.1016/j.ab.2016.07.022
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AccuBlue® NextGen dsDNA Quantitation Kit
RedDot™2 for Confocal Imaging of 3-D Tissue Architecture
van Royen ME, Verhoef EI, Kweldam CF, van Cappellen WA, Kremers GJ, Houtsmuller AB, van Leenders GJ. Three-dimensional microscopic analysis of clinical prostate specimens.Histopathology. 2016 Jun 29. doi: 10.1111/his.13022.
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RedDot™2 Far-Red Nuclear Stain, 200X in DMSO
Viability PCR for infectious norovirus in food and water samples
Randazzo, et al. Evaluation of viability PCR performance for assessing norovirus infectivity in fresh-cut vegetables and irrigation water. Int J Food Microbiol. 2016 Jul 16;229:106.
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PMAxx™ Dye, 20 mM in H2O
CF™488 for 2-photon microscopy
Mercier L, et al. In vivo imaging of skeletal muscle in mice highlights muscle defects in a model of myotubular myopathy. IntraVital. 2016 Apr 6;5(1):e1168553. doi: 10.1080/21659087.2016.1168553.
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α-Bungarotoxin, CF® Dye and Other Conjugates
EvaGreen® PCR for Point-of-Care Ebola Diagnosis
Ahrberg CD, Manz A, Neužil P. Palm-Sized Device for Point-of-Care Ebola Detection. Anal. Chem., 2016, 88 (9), pp 4803–4807. doi: 10.1021/acs.analchem.6b00278
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EvaGreen® Dye, 20X in Water